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Genotyping of Cryptosporidium spp. in environmental water in Turkey

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Abstract

This research was undertaken to study the molecular detection and characterization of Cryptosporidium spp. in environmental water sources at Samsun and Giresun Provinces of The Black Sea in Turkey. Two-hundred forty and one-hundred eighty environmental samples were collected from a total of twenty and twenty-five sampling sites of Giresun and Samsun Provinces. One hundred twenty untreated drinking water samples were also detected for Cryptosporidium spp. in both investigated areas. 101 (%42), 92 (%38.3) of 240 and 74 (41.1%), 70 (38.8%) of 180 environmental samples have been found positive for Cryptosporidium spp. by Loop mediated isothermal amplification (LAMP) targeting the S-adenosyl-L-methionine synthetase (SAM) gene and nested PCR targeting small subunit (SSU)rRNA gene in Samsun and Giresun Provinces, respectively. Of the tested untreated drinking water samples collected from the investigated area, one sample was positive for Cryptosporidium spp. Six and twelve samples were clearly sequenced for the Cryptosporidium (SSU)rRNA gene among the highest positive samples selected from each of the twenty and twenty-five sampling sites of Giresun and Samsun Provinces, respectively. Genetic characterization of Cryptosporidium isolates from water samples represented Cryptosporidium bovis for five samples, Cryptosporidium parvum for six samples and one sample for Cryptosporidium felis in Samsun Province, where C. parvum for five samples and C. bovis for one sample were sequenced in Giresun Province. According to accessible information sources, this is the first research about genotyping of Cryptosporidium spp. in water samples collected from Samsun and Giresun Provinces of Turkey.

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References

  • Bakheit M.A., Palomino L., Thekisoe O.M.M., Mate P.A., Ongerth J., Karanis P. 2008: Sensitive and specific detection of Cryptosporidium species in PCR-negative samples by loop-mediated isothermal DNA amplification and confirmation of generated LAMP products by sequencing. Veterinary Parasitology, 158, 11–22. DOI: 10.1016/j.vetpar.2008.09.012

    Article  CAS  Google Scholar 

  • Caccio S.M., Ryan U. 2008. Molecular epidemiology of giardiasis. Molecular and Biochemical Parasitology, 160, 75–80. DOI: 10.1016/j.molbiopara.2008.04.006

    Article  CAS  Google Scholar 

  • Castro-Hermida J.A., Garci’a-Presedo I., Almeida A., Gonza’lez-Warleta V.J., Correia Da Costa M., Mezo V. 2008. Contribution of treated wastewater to the contamination of recreational river areas with Cryptosporidium spp. and Giardia duodenalis. Water Research, 42, 3528–3538. DOI:10.1016/j.watres.2008.05.001

    Article  CAS  Google Scholar 

  • Fall A., Thompson R., Hobbs R., Ryan U. 2003. Morphology is not a reliable tool for delineating species within Cryptosporidium. Journal of Parasitology, 89, 399–402.DOI: 10.1645/0022-3395(2003)089[0399:MINART]2.0.CO;2

    Article  Google Scholar 

  • Francy D.S., Bushon R.N., Brady A.M.G., Bertke E.E., Kephart C.M., Likiardopulos C.A., Maillot B.E., Schaefer F.W., Lindquist A.H.D. 2009. Performance of traditional and molecular methods for detecting biological agents in drinking water. US Geological Survey Scientific Investigations Report 2009–5097

    Google Scholar 

  • Glaberman S., Moore J.E., Lowery C.J., Chalmers R.M., Sulaiman I., Elwin K., Rooney P.J., Millar B.C., Dooley J.S.G., Lal A.A., Xiao L. 2002. Three drinking-water-associated cryptosporidiosis outbreaks, Northern Ireland. Emerging Infectious Diseases, 8, 631–633. DOI: 10.3201/eid0806.010368

    Article  Google Scholar 

  • Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series. 41: 95–98

    CAS  Google Scholar 

  • Jiang J., Xiao L. 2003. An evaluation of molecular diagnostic tools for thedetection and differentiation of human pathogenic Cryptosporidium spp. Journal of Eukaryotic Microbiology, 50, 542–547. DOI: 10.1111/j.1550-7408.2003.tb00623.x

    Article  CAS  Google Scholar 

  • Josephine N.G., Yang R., Whiffin V., Cox P., Ryan U. 2011. Identification of zoonotic Cryptosporidium and Giardia genotypes infecting animals in Sydney’s water catchments. Experimental Parasitology, 128, 138–144

    Article  Google Scholar 

  • Karanis P., Thekisoe O., Kiouptsi K., Ongerth J., Igarashi I., Inoue N. 2007. Development and preliminary evaluation of loop-mediated isothermal amplification (LAMP) for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Applied and Environmental Microbiology, 73, 5660–5662. DOI: 10.1128/AEM.01152-07

    Article  CAS  Google Scholar 

  • Koksal F. 2002. Investigation of Source Waters for Giardia and Cryptosporidium. Journal of Turkish Society of Microbiology, 32, 275–277

    Google Scholar 

  • Koloren Z., Delioglu B.K. 2011. Prevalence of Cryptosporidium species in water supplies of Amasya, Middle Black Sea, by Acid-Fast staining methods. Journal of Applied Biological Sciences, 5, 81–84

    Google Scholar 

  • Koloren Z., Karanis P., Sotiriadou I. 2011. Investigations and Comparative Detection of Cryptosporidium Species by Microscopy, Nested PCR and LAMP in Water Supplies of Ordu, Middle Black Sea, Turkey. Annals of Tropical Medicine and Parasitology, 105, 607–615. DOI: 10.1179/2047773211Y.0000000011

    Article  CAS  Google Scholar 

  • Koloren Z., Kaya D., Avçar C. 2013. Detection of Cryptosporidium species in the sea and tap water samples of Black Sea, Turkey. Journal ofParasitology, 99, 554–557. DOI: 10.1645/GE-3232.1

    Article  CAS  Google Scholar 

  • Lobo M.L., Xiao L., Antunes F., Matos O. 2009. Occurrence of Cryptosporidium and Giardia genotypes and subtypes in raw and treated water in Portugal. Letters in Applied Microbiology, 48, 732–737. DOI: 10.1111/j.1472-765X.2009.02605.x

    CAS  PubMed  Google Scholar 

  • McLauchlin J., Amar C., Pedraza-Diaz S., Nichols G.L. 2000. Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in fecal samples from humans and fecal samples from livestock animals. Journal of Clinical Microbiology, 38, 3984–3990

    CAS  PubMed  PubMed Central  Google Scholar 

  • Minarovicová J., Kachkova E., Krascsenicsova K., Siekel P., Kuchta T. 2009. A single-tube nested real-time polymerase chain reaction for sensitive contained detection of Cryptosporidium parvum. Letters in Applied Microbiology, 49, 568–572. DOI:10.1111/j.1472-765X.2009.02708.x

    Article  Google Scholar 

  • Monis P.T., Saint C.P. 2001. Development of a nested-PCR assay for the detection of Cryptosporidium parvum in finished water. Water Research, 35, 1641–1648. DOI: 10.1016/S0043-1354(00)00426-7

    Article  CAS  Google Scholar 

  • Nichols R.A.B., Campbell B.M., Smith H.V. 2003. Identification of Cryptosporidium oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a modified nested PCR-restriction fragment length polymorphism assay. Applied and Environmental Microbiology, 69, 4183–4189. DOI: 10.1128/AEM.69.7.4183-4189.2003

    Article  CAS  Google Scholar 

  • Plutzer J., Karanis P., Domokos K., Torokne A., Marialigeti K. 2008. Detection and characterisation of Giardia and Cryptosporidium in Hungarian raw, surface and sewage water samples by IFT, PCR and sequence analysis of the SSUrRNA and GDH genes. International Journal of Hygiene and Environmental Health, 211, 524–533. DOI: 10.1016/j.ijheh.2008.04.004

    Article  CAS  Google Scholar 

  • Ryan U., Read C., Hawkins P., Warnecke M., Swanson P., Griffith M., Deere D., Cunningham M., Cox P. 2005. Genotypes of Cryptosporidium from Sydney water catchment areas. Journal of Applied Microbiology, 98, 1221–1229. DOI: 10.1111/j.1365-2672.2005.02562.x

    Article  CAS  Google Scholar 

  • Smith H.V., Caccio S.M., Tait A., Mc Lauchlin J., Thompson R.C.A. 2006. Tools for investigating the environmental transmission of Cryptosporidium and Giardia infections in humans. Trends in Parasitology, 22, 160–167. DOI: 10.1016/j.pt.2006.02.009

    Article  Google Scholar 

  • Spano F., Crisanti A. 2000. Cryptosporidiumparvum: The many secrets of a small genome. International Journal for Parasitology, 30, 553–565. DOI: 10.1016/S0020-7519(99)00188-5

    Article  CAS  Google Scholar 

  • Terzi G. 2005. Importance of Food-Borne Protozoal Infections for Public Health. Yuzuncu Yil University. Journal of Veterinary Medicine Faculty, 16, 47–55

    Google Scholar 

  • Xiao L. 2010. Molecular epidemiology of cryptosporidiosis: an update. Experimental Parasitology, 124, 80–89. DOI: 10.1016/j.exppara.2009.03.018

    Article  CAS  Google Scholar 

  • Xiao L., Alderisio K., Limor J., Royer M., Lal A.A. 2000. Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Applied and Environmental Microbiology, 66, 5492–5498. DOI: 10.1128/AEM.66.12.5492-5498.2000

    Article  CAS  Google Scholar 

  • Xiao L., Fayer R. 2008. Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. International Journal for Parasitology, 38, 1239–1255. DOI: 10.1016/j.ijpara.2008.03.006

    Article  CAS  Google Scholar 

  • Xiao L., Fayer R., Ryan U., Upton S.J. 2004. Cryptosporidium taxonomy: recent advances and implications for public health. Critical Reviews in Microbiology, 17, 72–97. DOI: 10.1128/CMR.17.1.72-97.2004

    Article  Google Scholar 

  • Xiao L., Ryan U.M. 2006. Molecular epidemiology. In: (Eds. Fayer, R. and Xiao, L.) Cryptosporidium and Cryptosporidiosis. CRS Press, Taylor & Francis Group, Boca Raton, 119–171

    Google Scholar 

  • Xiao L., Singh A., Limor J., Graczyk T.K., Gradus S., Lal A.A. 2001. Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater. Applied and Environmental Microbiology, 67, 1097–1101. DOI: 10.1128/ AEM.67.3.1097-1101.2001

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Faculty of Arts and Sciences, Ordu University, Ordu, Turkey

    Zeynep Koloren & Emine Ayaz

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  1. Zeynep Koloren
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  2. Emine Ayaz
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Correspondence to Zeynep Koloren.

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Koloren, Z., Ayaz, E. Genotyping of Cryptosporidium spp. in environmental water in Turkey. Acta Parasit. 61, 671–679 (2016). https://doi.org/10.1515/ap-2016-0094

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  • DOI: https://doi.org/10.1515/ap-2016-0094

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