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Acta Parasitologica

, Volume 61, Issue 4, pp 665–670 | Cite as

First report of mixed Entamoeba polecki (ST 1) and E. suis infection in piglets shedding abnormal feces by histopathological and molecular surveys

  • Makoto Matsubayashi
  • Yuri Sasagawa
  • Tsunehiko Aita
  • Masaharu Tokoro
  • Makoto Haritani
  • Tomoyuki ShibaharaEmail author
Article

Abstract

Of the three species of Entamoeba found in swine, namely E. histolytica, E. polecki and E. suis, E. histolytica can also infect humans, producing colitis or abscesses of liver and leading to death. However, the pathogenicity of other species of Entamoeba has not been fully characterized. Here, we conducted histopathology and molecular surveys on a pig farm where piglets had blackish feces or muddy diarrhea. Histopathological examination of two piglets showed necrosis of the mucous surface at the ileum, cecum, or colon, infiltration of neutrophils, and formation of ulcers. Based on morphological characteristics, E. polecki and E. suis trophozoites were mainly detected at lamina propria and surface of the lesion, respectively, and Lawsonia intracellularis, a bacterial pathogen, was also detected. Molecular analysis using the small subunit ribosomal RNA gene on other piglets and a sow revealed infection with both E. polecki and E. suis. These findings corroborate our previous reports that the two Entamoeba spp. are pathogenic in pigs as aggravations of symptoms with L. intracellularis. This is the first report about mixed infection with E. polecki and E. suis.

Keywords

Entamoeba suis Entamoeba polecki mixed infection pigs 

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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2016

Authors and Affiliations

  • Makoto Matsubayashi
    • 1
  • Yuri Sasagawa
    • 2
  • Tsunehiko Aita
    • 2
  • Masaharu Tokoro
    • 3
  • Makoto Haritani
    • 1
  • Tomoyuki Shibahara
    • 1
    Email author
  1. 1.National Institute of Animal HealthNational Agricultural and Food Research OrganizationTsukuba, IbarakiJapan
  2. 2.Chuou Animal Health and Hygiene Service CenterNiigata PrefectureNishikan-ku, NiigataJapan
  3. 3.Department of Parasitology, Graduate School of Medical SciencesKanazawa UniversityTakara-machi, KanazawaJapan

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