Acta Parasitologica

, Volume 60, Issue 2, pp 211–217 | Cite as

Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

  • Silvana CarnevaleEmail author
  • María Laura Pantano
  • Laura Kamenetzky
  • Jorge Bruno Malandrini
  • Claudia Cecilia Soria
  • Jorge Néstor Velásquez


Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.


Fasciola hepatica PCR faeces 


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Copyright information

© Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2015

Authors and Affiliations

  • Silvana Carnevale
    • 1
    • 2
    Email author
  • María Laura Pantano
    • 1
  • Laura Kamenetzky
    • 3
  • Jorge Bruno Malandrini
    • 4
  • Claudia Cecilia Soria
    • 4
  • Jorge Néstor Velásquez
    • 5
  1. 1.Instituto Nacional de Enfermedades Infecciosas - ANLIS “Dr. Carlos G. Malbrán”Ciudad de Buenos AiresArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Ciudad de Buenos AiresArgentina
  3. 3.Instituto de Microbiología y Parasitología Médica (IMPaM), UBA-CONICETCiudad de Buenos AiresArgentina
  4. 4.Facultad de Ciencias de la SaludUniversidad Nacional de CatamarcaSan Fernando del Valle de CatamarcaArgentina
  5. 5.Hospital Municipal de Infecciosas “Dr. Francisco Javier Muñiz”Ciudad de Buenos AiresArgentina

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