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Acta Parasitologica

, Volume 60, Issue 1, pp 65–74 | Cite as

Biological and molecular characterization of a Trypanosoma cruzi isolate obtained from Panstrongylus megistus captured in Sao Paulo State, Brazil

  • Luciamáre P. A. MartinsEmail author
  • Roberto E. P. Castanho
  • Altino L. S. Therezo
  • Aline R. Ribeiro
  • Luciana Lima
  • Marta M. G. Teixeira
  • Márcia A. Sperança
  • Vera L. C. Rodrigues
  • João A. da Rosa
Article

Abstract

An isolate of Trypanosoma cruzi obtained from P. megistus captured in the peridomicile area of a home in Santo Antonio do Jardim city in the State of São Paulo, denominated T. cruzi Mogi, was characterized biologically and molecularly. The RFLP analysis of the D7 divergent domain in the 24Sα rDNA and of the mini-exon positioned the T. cruzi isolate within the TcI group. Phylogenetic analysis performed with the trypanosomatid barcode confirmed that the isolate belongs to the TcI group, with high homology to the 3014 c1 T.cruzi strain. The biological characterization of the isolate in rats showed a prepatent period of about 8 days, low parasitemia and tropism for cardiac, skeletal and colonic muscles. In Swiss mice the T. cruzi Mogi isolate showed a prepatent period of about 22 days, intermittent parasitemia in some animals, and tropism for cardiac and colonic muscles. Despite the inherent difficulty of identifying correlations amongst the molecular and biological characteristics of different T. cruzi groups, the tropism for colonic muscle demonstrated by T. cruzi Mogi represented a peculiarity of this isolate within the TcI group.

Keywords

Trypanosoma cruzi Chagas’ disease characterization lineage DNA strains 

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Copyright information

© W. Stefański Institute of Parasitology, PAS 2015

Authors and Affiliations

  • Luciamáre P. A. Martins
    • 1
    Email author
  • Roberto E. P. Castanho
    • 1
  • Altino L. S. Therezo
    • 2
  • Aline R. Ribeiro
    • 7
  • Luciana Lima
    • 4
  • Marta M. G. Teixeira
    • 4
  • Márcia A. Sperança
    • 5
  • Vera L. C. Rodrigues
    • 6
  • João A. da Rosa
    • 3
  1. 1.Disciplina de Parasitologia da Faculdade de Medicina de MaríliaBrazil
  2. 2.Disciplina de Anatomia Patológica da Faculdade de Medicinade MaríliaBrazil
  3. 3.Disciplina de Parasitologia da Faculdade de Ciências Farmacêuticas da Unesp de AraraquaraBrazil
  4. 4.Instituto de Ciências Biomédicas da Universidade de São PauloBrazil
  5. 5.Centro de Ciencias Naturais e HumanasUniversidade Federal do ABCBrazil
  6. 6.Superintendência de Controle de Endemias de Mogi-GuaçuBrazil
  7. 7.Departamento de Biologia Animal do Instituto de BiologiaUniversidade Estadual de CampinasBrazil

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