Abstract
OBJECTIVE
As we previously demonstrated, the inhibitory effect of iodine on thyroid cell growth is mediated by iodolactones, especially 6-iodo-5-hydroxy-eicosatrienoic acid (δ-iodolactone). In this communication we compare the effect of iodide, molecular iodine and δ-iodolactone on growth inhibition and apoptosis on three human thyroid carcinoma cell lines (B-CPAP cells, FTC-133 cells and 8505C cells) as well as on human breast cancer cells (MCF 7).
METHODS
Thyroid carcinoma cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) and MCF 7 cells in Rowswell Park Memorial Institute (RPMI) culture medium, both containing 10% (v/v) Fetal Calf Serum (FCS), until they were confluent. Around 2000 cells were then distributed in 12-well plates and grown for 48 h in either DMEM (thyroid cancer cells) or in RPMI medium (MCF 7 cells) both containing 5% FCS. Thereafter, different concentrations of iodide, iodine or δ-iodolactone were added for 24 h. Growth rate was estimated by cell counting in a Coulter Counter adapted for epithelial cells. Apoptosis was determined by a mitochondrial potential assay.
RESULTS
The growth rate of B-CPAP cells was unaffected by iodide, but was reduced by high concentreations of molecular iodine (100 and 500 µM). However, δ-iodolactone significantly reduced cell proliferation already with low concentrations (5 µM and 10 µM) and further in a dose-dependent manner up to 82%. FTC-133 and 8505C cells were unaffected by iodide, iodine or δ-iodolactone. In contrast, in MCF 7 cells, molecular iodine (100 µM) inhibited growth from 100% to 83% but δ-iodolactone (1, 5 and 10 µM) dose-dependently decreased growth rate from 100% to 82% and 62%, respectively. The inhibition of growth was through apoptosis, and not necrosis, as the amount of apoptotic cells corresponded to the growth inhibition.
CONCLUSION
δ-?odolactone seems to be the main iodocompound which can inhibit growth and induce apoptosis in B-CPAP cells as well as in MCF 7 breast cancer cells.
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Gärtner, R., Rank, P. & Ander, B. The role of iodine and δ-iodolactone in growth and apoptosis of malignant thyroid epithelial cells and breast cancer cells. Hormones 9, 60–66 (2010). https://doi.org/10.14310/horm.2002.1254
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DOI: https://doi.org/10.14310/horm.2002.1254