Effects of a Six-Month Multi-Ingredient Nutrition Supplement Intervention of Omega-3 Polyunsaturated Fatty Acids, vitamin D, Resveratrol, and Whey Protein on Cognitive Function in Older Adults: A Randomised, Double-Blind, Controlled Trial
To investigate the impact of a six-month multi-ingredient nutrition supplement intervention (Smartfish®), containing omega-3 polyunsaturated fatty acids (PUFAs), vitamin D, resveratrol, and whey protein, on cognitive function in Irish older adults.
Double-blind, randomised controlled trial (ClinicalTrials.gov: NCT02001831). A quantitative, mixedmodel design was employed in which the dependent variable (cognitive function) was analysed with a between-subjects factor of group (placebo, intervention) and within-subjects factor of testing occasion (baseline, three-months, six-months).
Community-based intervention including assessments conducted at University College Dublin, Ireland.
Thirty-seven community-dwelling older adults (68-83 years; mean (x̅)= 75.14 years; standard deviation (SD)= 3.64; 18 males) with normal cognitive function (>24 on the Mini Mental State Examination) were assigned to the placebo (n= 17) or intervention (n= 20) via a block randomisation procedure.
Daily consumption for six-months of a 200mL liquid juice intervention comprising 3000mg omega-3 PUFAs [1500mg docosahexaenoic acid (DHA) and 1500mg eicosapentaenoic acid (EPA)], 10μg vitamin D3, 150mg resveratrol and 8g whey protein isolate. The placebo contained 200mL juice only.
A standardised cognitive assessment battery was conducted at baseline and follow-ups. Individual test scores were z-transformed to generate composite scores grouped into cognitive domains: executive function, memory, attention and sensorimotor speed. Motor imagery accuracy and subjective awareness of cognitive failures variables were computed from raw scores.
A hierarchical statistical approach was used to analyse the data; first, by examining overall cognitive function, then by domain, and then by individual test scores. Using mixed between-within subjects, analyses of variance (ANOVAs), no significant differences in overall cognitive function or composite cognitive domains were observed between groups over time. The only significant interaction was for Stroop Color-Word Time (p< 0.05). The intervention group demonstrated reduced task completion time at three- and six-month follow-ups, indicating enhanced performance.
The present nutrition intervention encompassed a multi-ingredient approach targeted towards improving cognitive function, but overall had only a limited beneficial impact in the older adult sample investigated. Future investigations should seek to establish any potential clinical applications of such targeted interventions with longer durations of supplementation, or in populations with defined cognitive deficits.
Key wordsCognitive failures executive function aging nutrition supplementation
Abbreviations and Symbols
Analysis of Variance
Auditory Verbal Learning Test
Body Mass Index
Cognitive Failures Questionnaire
Controlled Oral Word Association
Mini Mental State Examination
Polyunsaturated fatty acid
Randomised controlled trial
Trail Making Test
Timed Up and Go
University College Dublin
Wechsler Adult Intelligence Scale III
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- 1.Johansson B. Memory and cognition in aging. In: Woods R, Clare L (eds) Handbook of the clinical psychology of aging, 2nd edn. John Wiley & Sons LTD, New York, 2008;pp 33–55.Google Scholar
- 3.Sydenham E, Dangour AD, Lim WS. Omega 3 fatty acid for the prevention of cognitive decline and dementia. Cochrane Database of Syst Rev 2012;13(6):1–42. doi:10.1002/14651858.CD005379.pub3.Google Scholar
- 4.Gillette Guyonnet S, Abellan van Kan G, Andrieu S, Barberger Gateau P, Berr C, Bonnefoy M, Dartigues JF, de Groot L, Ferry M, Galan P, Hercberg S, Jeandel C, Morris MC, Nourhashemi F, Payette H, Poulain JP, Portet F, Roussel AM, Ritz P, Rolland Y, Vellas B. IANA task force on nutrition and cognitive decline with aging. J Nutr Health Aging 2007;11:132–152.PubMedGoogle Scholar
- 6.Hooijmans CR, Pasker-de Jong PC, de Vries RB, Ritskes-Hoitinga M. The effects of long-term omega-3 fatty acid supplementation on cognition and Alzheimer’s pathology in animal models of Alzheimer’s disease:a systematic review and meta-analysis. J Alzheimers Dis 2012;28:191–209. doi:10.3233/JAD-2011-111217.CrossRefPubMedGoogle Scholar
- 10.Dangour AD, Allen E, Elbourne D, Faey N, Fletcher AE, Hardy P, Holder GE, Knight R, Letley L, Richards M, Uauy R. Effect of 2-y n-3 long-chain polyunsaturated fatty acid supplementation on cognitive function in older people:a randomised, double-blind, controlled trial. Am J Clin Nutr 2010;91:1725–1732. doi:10.3945/ajcn.2009.29121.CrossRefPubMedGoogle Scholar
- 12.van de Rest O, Geleijnse JM, Kok FJ, van Staveren WA, Dullemeijer C, Olderikkert MGM, Beekman AT, de Groot CP. Effect of fish oil on cognitive performance in older subjects; A randomised, controlled trial. Neurology 2008;71:430–438. doi:10.1212/01.wnl.0000324268.45138.86.CrossRefPubMedGoogle Scholar
- 18.Slinin Y, Paudel ML, Taylor BC, Fink HA, Ishani A, Canales MT, Yaffe K, Barrett-Connor E, Orwoll ES, Shikany JM, Leblanc ES, Cauley JA, Ensrud KE, Osteoporotic Fractures in Men (MrOS) Study Research Group. 25-Hydroxyvitamin D levels and cognitive performance and decline in elderly men. Neurology 2010;74:33–41. doi:10.1212/WNL.0b013e3181c7197b.PubMedGoogle Scholar
- 20.Army Individual Test Battery. Manual of directions and scoring. War Department, Adjutant General’s Office, Washington DC, 1944.Google Scholar
- 26.Bauer JM, Verlaan S, Bautmans I, Brandt K, Donini LM, Maggio M, McMurdo ME, Mets T, Seal C, Wijers SL, Ceda GP, De Vito G, Donders G, Drey M, Greig C, Holmbäck U, Narici M, McPhee J, Poggiogalle E, Power D, Scafoglieri A, Schultz R, Sieber CC, Cederholm T. Effects of a vitamin D and leucineenriched whey protein nutritional supplement on measures of sarcopenia in older adults, the PROVIDE Study:A randomized, double-blind, placebocontrolled trial. J Am Med Dir Assoc 2015;16(9):740–747. doi:10.1016/j. jamda.2015.05.021.CrossRefPubMedGoogle Scholar
- 27.Weaver CM. Role of dairy beverages in the diet. Physiol Behav 100:2010;63–66. doi:10.1016/j.physbeh.2010.01.020.Google Scholar
- 32.Rey A. The clinical examination in psychology. Universitaires de France, Paris, 1964.Google Scholar
- 34.Trenerry M, Crosson B, DeBoe J, Leber W. Stroop neuropsychological screening test manual. Psychological Assessment Resources (PAR), Adessa, Florida, 1989.Google Scholar
- 35.Benton AL, Hamsher KD. Multilingual aphasia examination. AJA Associates, Iowa City, Iowa, 1989.Google Scholar
- 36.Wechsler D. Wechsler adult intelligence scale, 3rd edn. Harcourt Assessment, San Antonio, Texas, 1997Google Scholar
- 39.Beauchet O, Launay CP, Sejdic E, Allali G, Annweiler C. Motor imagery of gait:a new way to detect mild cognitive impairment? J Neuroeng Rehabil 2014;11(66):1–7. doi:10.1186/1743-0003-11-66.Google Scholar
- 41.IBM SPSS Statistics. IBM SPSS statistics 20.0 for Windows/Apple Mac. SPSS Inc, Chicago, 2011.Google Scholar
- 43.Famenini S, Rigali EA, Olivera-Perez HM, Dang J, Chang MT, Halder R, Rao RV, Pellegrini M, Porter V, Bredesen D, Fiala M. Increased intermediate M1-M2 macrophage polarization and improved cognition in mild cognitive impairment patients on omega-3 supplementation. FASEB J 2017;31:148–160. doi:10.1096/fj.201600677RR.CrossRefPubMedGoogle Scholar