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NanoBiotechnology

, Volume 1, Issue 4, pp 353–360 | Cite as

Single-wall carbon nanotubes with adsorbed antibodies detect live breast cancer cells

  • Balaji Panchapakesan
  • Gregory Cesarone
  • Shaoxin Liu
  • Kasif Teker
  • Eric Wickstrom
Original Article

Abstract

Monoclonal antibodies (mAb) specific to cell surface antigens overexpressed on cancer cells adsorbed to single-wall carbon nanotube (SWCNT) devices can bind to their antigens in a drop of buffer, resulting in a slight drop in conductance. Here we report detection of live breast cancer cells with a mAb-SWCNT device. We adsorbed mAb specific to insulin-like growth factor 1 receptor (IGF1R) onto interconnected SWCNT networks placed between lithographically patterned electrodes. Application of human BT474 breast cancer cells increased the conductance of the IGF1R-specific mAb-SWCNT devices by 3.0±0.1-fold, relative to nanotube devices with non specific mAb. Human MCF7 breast cancer cells, with greater IGF1R expression, increased the conductance by 8.0±0.2-fold, but R-cells lacking IGF1R did not. Receptor-specific mAb acted as specific nanoswitches that completed a circuit between the SWCNT and the cell surface receptors, elevating the device conductance. Such devices might detect circulating breast cancer cells in blood samples.

Key Words

Antibodies conductance Her2 IGF1 receptor single-wall carbon nanotube 

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Copyright information

© Humana Press Inc 2005

Authors and Affiliations

  • Balaji Panchapakesan
    • 1
  • Gregory Cesarone
    • 2
  • Shaoxin Liu
    • 1
  • Kasif Teker
    • 1
  • Eric Wickstrom
    • 2
    • 3
  1. 1.Delaware MEMS and Nanotechnology Laboratory, Department of Electrical and Computer EngineeringUniversity of DelawareNewark
  2. 2.Department of Biochemistry and Molecular BiologyThomas Jefferson UniversityPhiladelphia
  3. 3.Department of Biochemistry and Molecular Biology, Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphia

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