Medical Oncology

, Volume 23, Issue 1, pp 105–112 | Cite as

In vivo and in vitro antitumor effect of ascorbic acid, lysine, proline, arginine, and green tea extract on human fibrosarcoma cells HT-1080

  • M. Waheed Roomi
  • Vadim Ivanov
  • Tatiana Kalinovsky
  • Aleksandra Niedzwiecki
  • Matthias Rath
Original Article

Abstract

Current treatment of fibrosarcoma, an aggressive cancer of the connective tissue, is generally associated with poor prognosis. Matrix metalloproteinases (MMPs), vascular endothelial growth factor (VEGF), and constituents of the extracellular matrix (ECM), such as fibronectin, play a critical role in angiogenesis and underlie neoplastic invasion and metastasis. This and anticancer properties of lysine, proline, arginine, ascorbic acid, and green tea extract (NM) prompted us to investigate the effect of these nutrients in vitro on human fibrosarcoma cells HT-1080 by measuring cell proliferation, modulation of MMP-2 and MMP-9, and invasive potential. In vivo, we studied the growth of human fibrosarcoma HT-1080 cells in athymic nude mice and the expression of MMPs and VEGF. Cell proliferation was evaluated by MTT assay, MMP expression by gelatinase zymography, and invasion through Matrigel and migration by scratch assay. Tumors were excised, weighed, and processed for histology in both the control and nutrient-supplemented groups. Results showed NM inhibited the growth and reduced the size of tumors in nude mice; decreased MMP-9 and VEGF secretion was found in the suplemented group tissues. NM inhibited invasion through Matrigel and migration with total inhibition at 1000 μg/mL. These results offer promise in the therapeutic use of the nutrient mixture of lysine, proline, arginine, ascorbic acid, and green tea extract tested in the treatment of fibrosarcoma.

Key words

Fibrosarcoma xenograft nude mice green tea extract ascorbic acid lysine proline antitumor effect MMP-9 Matrigel invasion 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • M. Waheed Roomi
    • 1
  • Vadim Ivanov
    • 1
  • Tatiana Kalinovsky
    • 1
  • Aleksandra Niedzwiecki
    • 1
  • Matthias Rath
    • 1
  1. 1.Matthias Rath Research InstituteCancer DivisionSanta Clara

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