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Medical Oncology

, Volume 20, Issue 2, pp 175–180 | Cite as

Protective effect of amifostine against cisplatin-induced motor neuropathy in rat

  • Suayib Yalcin
  • Gulay Nurlu
  • Bülent Orhan
  • Dilara Zeybek
  • Sevda Müftüoğlu
  • Banu Şarer
  • Berna Akkuş Yildirim
  • Eren Cetin
Original Article

Abstract

Cisplatin (CDDP) is a potent anticancer drug. Neurotoxicity is one of the most important dose-limiting toxicity of CDDP. We investigated the role of amifostine in the protection against CDDP-induced neurotoxicity especially on the motor nerves. All experiments were conducted on female Wistar albino rats. Animals were randomly assigned to two groups, each including six rats. Group A received CDDP plus amifostine and Group B received CDDP only. Electroneurography (ENG) was carried out in the beginning and at the end of 7 wk; then, the rats were sacrificed and the sciatic nerve was removed for histopathological examination.

The mean initial latency was 2.4667 msn for group A and 2.44833 msn for group B. After 7 wk of treatment, the latency was 2.9167 for group A and 2.6333 for group B. The difference in latencies was not statistically significant. The amplitude was 11.7853 mV and 13.533 mV for groups A and B, respectively. After 7 wk of treatment, the amplitude was 9.400 mV and 9.000 mV, respectively. The decrease of amplitude in compound muscle action potential (CMAP) was 20% in the amifostine group and the decrease was 33% in the untreated group. The mean area of the CMAP in group A was 9.400 mVsn initially and 9.666 mVsn at the end of the treatment; there was a 0.3% increase despite CDDP treatment. In group B, the mean area of the CMAP was 13.816 mVsn initially and 11.857 mVsn at the end of the treatment; this corresponded to a statistically significant 14% decrease as a result of CDDP treatment. The ENG and histopathological studies showed that at the given dose and schedule CDDP-induced motor neuropathy and amifostine reduced this neuropathy both by protection of the amplitude and area of the CMAP in ENG studies and by sparing a larger number of nerve fibers.

Key Words

Neurotoxicity cisplatin amifostine 

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Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  • Suayib Yalcin
    • 1
  • Gulay Nurlu
    • 2
  • Bülent Orhan
    • 6
  • Dilara Zeybek
    • 3
  • Sevda Müftüoğlu
    • 3
  • Banu Şarer
    • 4
  • Berna Akkuş Yildirim
    • 5
  • Eren Cetin
    • 5
  1. 1.Institute of OncologyHacettepe UniversityAnkaraTurkey
  2. 2.Department of NeurologyHacettepe UniversityAnkaraTurkey
  3. 3.Department of Histology and EmbryologyHacettepe UniversityAnkaraTurkey
  4. 4.Department of Internal MedicineHacettepe UniversityAnkaraTurkey
  5. 5.Department of Radiation OncologyHacettepe UniversityAnkaraTurkey
  6. 6.Ali Osman Sönmez Oncology HospitalBursaTurkey

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