Clinical significance of nm23 expression and chromosome 17 numerical aberrations in primary gastric cancer
The metastasis suppressor gene nm23 located on chromosome 17 might be one of the targets in deletions of chromosome 17. In this study, we analyzed the expression of nm23 and chromsome 17 aberrations in gastric cancer and assessed their clinicopathological and prognostic significance. In 103 gastric cancer patients, we examined nm23 expression by immunohistochemistry and detected chromosome 17 aberrations by fluorescence in situ hybridization. There was a significant difference in the expression of nm23 among differentiated histologic types (well>moderately>poorly) (p<0.01). Negative expression of nm23 correlated with depth of invasion (p<0.01), lymph node metastasis (p<0.05), lymphatic invasion (p<0.05), venous invasion (p<0.05), poor prognosis (p<0.05), and chromosome 17 loss (p<0.05). Chromosome 17 aberrations broadly correlated with clinicopathological variables and were associated with poor prognosis (p<0.05). Univariate analyses identified nm23 (p<0.05), chromosome 17 aberrations (p<0.05), tumor size (p<0.01), depth of invasion (p<0.0001), lymph node metastasis (P<0.001), hepatic metastasis (p<0.01), peritoneal dissemination (p<0.01), and lymphatic invasion (p<0.01) as significant prognostic factors. Multivariate analysis showed that expression of nm23 and chromosome 17 aberrations were not independent prognostic indicators. Our results indicate that negative expression of nm23 and chromosome 17 numerical aberrations correlate with tumor progression and poor prognosis but are not independent prognostic indicators.
Key WordsGastric cancer nm23 chromosome 17 immunohistochemistry fluorescence in situ hybridization (FISH)
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- 4.Gomyo, Y., Osaki, M., Kaibara, N. and Ito, H. (1996). Numerical aberration and point mutation of p53 gene in human gastric intestinal metaplasia and well-differentiated adenocarcinoma: analysis by fluorescence in situ hybridization (FISH) and PCR-SSCP. Int. J. Cancer 66:594–599.PubMedCrossRefGoogle Scholar
- 12.Bevilacqua, G., Sobel, M.E., Liotta, L.A. and Steeg, P.S. (1989). Association of low nm23 RNA levels in human primary infiltrating ductal breast carcinoma with lymph node involvement and other histopathological indicators of high tumor metastatic potential. Cancer Res. 49:5185–5190.PubMedGoogle Scholar
- 15.Mandai, M., Konishi, I., Koshiyama, M., Mori, T., Arao, S., Tashiro, H., et al. (1994). Expression of metastasis-related nm23-H1 and nm23-H2 genes in ovarian carcinomas: correlation with clinicopathology, EGFR, c-erbB-2, and c-erbB-3 genes, and sex steroid receptor expression. Cancer Res. 54:1825–1830.PubMedGoogle Scholar
- 17.Japanese Gastric Cancer Association (1999). Japanese Classification of Gastric Carcinoma, 13th ed., pp1–155, Kanehara, Tokyo (in Japanese).Google Scholar