Analysis of immune response in young and aged mice vaccinated with corn-derived antigen against Escherichia coli heat-labile enterotoxin
- 144 Downloads
Enterotoxigenic strains of Escherichia coli produce a heat-labile holotoxin (LT), which causes diarrhea. We engineered corn seeds to produce LT-B, the nontoxic subunit of LT, to serve as a plant-derived vaccine to traveler's diarrhea and as an adjuvant for co-administered proteins. We previously demonstrated that a strong mucosal and systemic antibody response is elicited in young mice with oral administration of corn-derived LT-B. The present study examined systemic and mucosal antibody responses to LT-B in young and aged mice, and recall responses to oral administration and injection of LT-B in aged mice. Specific IgA and IgG antibodies were detectable during an 11-mo period, although the concentration of antigen-specific antibodies declined gradually. Booster by feeding or injection dramatically increased the concentration of specific IgA from that seen in young mice. Specific IgG levels were boosted to concentrations similar to those in young mice. This effect may be age-dependent and related to prior immunization exposure. Analysis of the antibody response of naive aged mice against corn-derived LT-B demonstrated an age-related suppression in specific IgG production, but not specific IgA. These results may provide important information for edible vaccine strategies for young and aged individuals.
Index EntriesPlant-derived vaccines immune memory LT-B
Unable to display preview. Download preview PDF.
- 1.McGhee, J. R. and Kyono, H. (1998) Mucosal immunology. In Fundamental Immunology (Paul, W. E., ed.) San Diego, Academic Press, pp. 909–945.Google Scholar
- 7.Kanellos, T. S., Byarugaba, D. K., Russell, P. H., Howard, C. R., and Partidos, C. D. (2000) Naked DNA when co-administered intranaslly with heatlabile enterotoxin of Escherichia coli primes effectively for systemic B- and T-cell responses to the encoded antigen. Immunol. Lett. 74, 215–220.PubMedCrossRefGoogle Scholar
- 15.Verweij, W. R., Haan, L., Holtrop, M., et al. (1998) Mucosal immunoadjuvant activity of Escherichia coli heat-labile enterotoxin and its B subunit: induction of systemic IgG and secretory IgA responses in mice by intranasal immunization with influenza virus surface antigen. Vaccine 16, 2069–2076.PubMedCrossRefGoogle Scholar
- 18.Guidry, J. J., Cardenas, L., Cheng, E., and Clements, J. D. (1997) Role of receptor binding in toxicity, immunogenicity, and adjuvanticity of Escherichia coli heat-labile enterotoxin. Infect. Immunol. 65, 4943–4950.Google Scholar
- 22.Nedrud, J. G. and Sigmund, N. (1991) Cholera toxin as a mucosal adjuvant: II. Antibody responses to non-target dietary antigens are not increased. Regional Immunol. 3, 217–222.Google Scholar
- 29.Clements, J. D. (1990) Construction of a nontoxic fusion peptide for immunization against Escherichia coli strains that produce heat-labile and heat-stable enterotoxins. Infect. Immunol. 58, 1159–1166.Google Scholar
- 35.Schmuker, D. and Owen, R. (1997) Aging and gastrointestinal mucosal immune response. Mech. Ageing Dev. 13, 534–541.Google Scholar
- 37.Arranz, E. and Ferguson, A. (1992) Aging and gastrointestina immunity. Gastroenterol. Hepatol. 4, 1–14.Google Scholar
- 38.Beherkaka, A. A., Paiva, S., Leka, L. S., Ribaya-Mercado, J. D., Russell, R. M., and Meydani, S. N. (2001) Effect of age on the gastrointestinal-associated mucosal immune response of humans. J. Geronol. 56A, B218-B223.Google Scholar