Molecular Biotechnology

, Volume 28, Issue 3, pp 195–199 | Cite as

Knockdown of mouse adult β-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference

  • Na Zhao
  • Zhen-Xiang Zu
  • Chang-Mei Liu
  • Wen-Ji Dong
  • De-Pei Liu
  • Chih-Chuan Liang
Research

Abstract

RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21–23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especially when it is necessary to generate long-term gene silencing in vivo. Virus vector-mediated RNAi provides an alternative to transfection. In the present study, we investigated such transduction system and showed that retrovirus vector-mediated RNAi can substantially down-regulate expression of mouse adult β-globin gene in MEL cells. The results suggest that retrovirus vector-delivered RNAi may find its use in functional genomics and in gene therapy.

Index Entries

Mouse adult β-globin gene retrovirus vector RNA interference 

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Copyright information

© Humana Press Inc 2004

Authors and Affiliations

  • Na Zhao
    • 1
  • Zhen-Xiang Zu
    • 1
  • Chang-Mei Liu
    • 1
  • Wen-Ji Dong
    • 1
  • De-Pei Liu
    • 1
  • Chih-Chuan Liang
    • 1
  1. 1.National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC)BeijingP. R. China

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