Knockdown of mouse adult β-globin gene expression in MEL cells by retrovirus vector-mediated RNA interference
RNA interference (RNAi) efficiently induces sequence-specific gene silencing in mammalian cells through short interfering RNA (siRNA) of 21–23 nucleotides synthesized in vitro or expressed by DNA-based vector. However, introduction of siRNA into mammalian cells by transfection limits the application of RNAi, especially when it is necessary to generate long-term gene silencing in vivo. Virus vector-mediated RNAi provides an alternative to transfection. In the present study, we investigated such transduction system and showed that retrovirus vector-mediated RNAi can substantially down-regulate expression of mouse adult β-globin gene in MEL cells. The results suggest that retrovirus vector-delivered RNAi may find its use in functional genomics and in gene therapy.
Index EntriesMouse adult β-globin gene retrovirus vector RNA interference
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- 7.Yokota, T., Sakamoto, N., Enomoto, N., Tanabe, Y., Miyagishi, M., Maekawa, S., Yi L., Kurosaki, M., Taira, K., Watanabe, M., and Mizusawa, H. (2003) Inhibition of intracellular hepatitis C virus replication by synthetic and vector-derived small interfering RNAs. EMBO Rep. 4, 602–608.PubMedCrossRefGoogle Scholar
- 11.Rubinson, D. A., Dillon, C. P., Kwiatkowski, A. V., Sievers, C., Yang, L., Kopinja, J., Zhang, M., McManus, M. T., Gertler, F. B., Scott, M. L., and Parijs, L. V. (2003) A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells, transgenic mice by RNA interference. Nat. Genet. 33, 401–406.PubMedCrossRefGoogle Scholar