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Molecular Biotechnology

, Volume 25, Issue 1, pp 95–99 | Cite as

Preparation of peptide-loaded dendritic cells for cancer immunotherapy

  • Michael A. Morse
  • Tim Clay
  • Kirsten Colling
  • H. Kim Lyerly
Protocol

Abstract

Dendritic cell-based vaccines are being evaluated in clinical trials to determine their ability to activate clinically relevant tumor antigen-specific immune responses. Although some groups isolate dendritic cells from peripheral blood, most have found it more efficient to generate large numbers from peripheral blood progenitors, particularly plastic adherent or CD14+ monocytes, in media supplemented with GM-CSF and IL-4. These DC may then be matured, if desired, and loaded with antigen, such as tumor-associated peptides, prior to administration. We describe the scheme that we are currently using to generate peptide-loaded dendritic cells for our clinical trials of cancer immunotherapy.

Index Entries

Dendritic cells cancer immunotherapy 

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Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  • Michael A. Morse
    • 1
  • Tim Clay
    • 2
  • Kirsten Colling
    • 2
  • H. Kim Lyerly
    • 2
  1. 1.Department of MedicineDuke University Medical centerDurham
  2. 2.Department of SurgeryDuke University Medical CenterDurham

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