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Molecular Biotechnology

, Volume 21, Issue 1, pp 43–50 | Cite as

Differential gene expression during somatic embryogenesis in Coffea arabica L., revealed by RT-PCR differential display

  • R. Rojas-Herrera
  • F. Quiroz-Figueroa
  • M. Monforte-González
  • L. Sánchez-Teyer
  • V. M. Loyola-Vargas
Research

Abstract

Molecular and biochemical studies of somatic embryogenesis may help to shed light on the mechanisms governing this phenomenon. In this article, a differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Cloned fragments show homologies to several proteins reported in databases, but only one has previously been described as regulated during somatic embryogenesis. By a reverse dot blot modification, the expression pattern of such fragments was evaluated.

Index Entries

Coffea arabica somatic embryogenesis gene expression differential display 

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Copyright information

© Humana Press Inc 2002

Authors and Affiliations

  • R. Rojas-Herrera
    • 1
    • 2
  • F. Quiroz-Figueroa
    • 1
  • M. Monforte-González
    • 1
  • L. Sánchez-Teyer
    • 1
  • V. M. Loyola-Vargas
    • 1
  1. 1.Unidad de Bioquímica y Biología Molecular de PlantasCentro de Investigación Científica de YucatánCordemex, Mérida, YucatánMéxico
  2. 2.Departamento de Genética y MejoramientoInstituto Nacional de Ciencias Agrícolas. Gaveta Postal 1. San José de las LajasLa HabanaCuba

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