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Molecular Biotechnology

, Volume 19, Issue 2, pp 201–203 | Cite as

Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit

  • Laura Jaakola
  • Anna Maria Pirttilä
  • Minna Halonen
  • Anja Hohtola
Protocol

Abstract

A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and β-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.

Index Entries

RNA isolation CTAB fruit tissue bilberry Vaccinium myrtillus 

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Copyright information

© Humana Press Inc 2001

Authors and Affiliations

  • Laura Jaakola
    • 1
  • Anna Maria Pirttilä
  • Minna Halonen
  • Anja Hohtola
  1. 1.Department of BiologyUniversity of OuluOuluFinland

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