Advertisement

Molecular Biotechnology

, Volume 16, Issue 2, pp 161–173 | Cite as

Plant cell culture initiation

Practical tips
Protocol

Abstract

The use of cultured plant cells in either organized or unorganized form has increased vey considerably in the last 10–15 yr. Many new technologies have been developed and applications in both fundamental and applied research have led to the development of some powerful tools for improving our knowledge of botanical systems and for gaining external influence over some of the key processes involved in inter-and intracellular organization. This is particularly the case when cell culture techniques are combined with those for the genetic modification of plant cells. Being able to regenerate whole plants that have gained or lost the expression of one or more specific genes has revolutionized the way in which we approach scientific questions and has opened up many additional possibilities for the molecular dissection of plants.

The success or fall of all plant cell culture technologies lies with culture initiation. The choice of plant material, its physiologival state and cultivation history, the media used, and their means of preparation are just some of the factors that can greatly influence whether the desired end result will be achieved. In this article are described some of the practical aspects involved in successful plant cell culture initiation and the choices that have to be made. Attention is given to some of the pitfalls that can occur and how to avoid them. A good start is half the work

Index Entries

Plant cell culture: technology development practical tips 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    George, E. F. (1993) Plant Propagation by Tissue Culture I: The technology. Exegetics Ltd, Edington, UK.Google Scholar
  2. 2.
    Martinez-Zapater, J. M., Salinas, J. (1998) Arabidopsis Protocols. Humana Press, Totowa, NJ.Google Scholar
  3. 3.
    Street, H. E. (ed.) (1974) Tissue Culture and Plant Science. Academic Press, New York.Google Scholar
  4. 4.
    Pierik, R. L. M. (1997) In Vitro Culture of Higher Plants. Kluwer, Dordrecht, The Netherlands.Google Scholar
  5. 5.
    Giles, K. L. and Friesen, K. R. D. (1994) Micropropagation, in Biotechnological Applications of Plant Culture, Shargool, P. D. and Ngo, T. T. (eds.) CRC Press, Boca Raton, FL, pp. 111–128.Google Scholar
  6. 6.
    Jones, H. (1995) Plant Gene Transfer and Experimental Protocols. Humana Press, Totowa, NJ.Google Scholar
  7. 7.
    Christou, P. (1995) Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment. Euphytica 85, 13–27.CrossRefGoogle Scholar
  8. 8.
    Dodds, J. H. and Robert L. W. (1982) Experiments in Plant Tissue Culture. Cambridge University Press, Cambridge, UK.Google Scholar
  9. 9.
    Brown, D. C. W., Thorpe, T. A. (1984) Organisation of a plant tissue culture laboratory. In Vasil, I. K., (ed.) Cell Culture and Somatic Cell Genetics, Academic Press, Orlando, FL, pp. 1–12.Google Scholar
  10. 10.
    Gamborg, O. L. (1991) Media preparation, in Plant Tissue Culture Manual A1, (Lindsey, K., ed.), Kluwer, Dordrecht, The Netherlands, pp. 1–24.Google Scholar
  11. 11.
    Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–479.CrossRefGoogle Scholar
  12. 12.
    Linsmaier, E. M. and Skoog, F. (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127.CrossRefGoogle Scholar
  13. 13.
    Gamborg, O. L., Miller, R. A., and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50, 151–157.PubMedCrossRefGoogle Scholar
  14. 14.
    Schenk, R. U. and Hildebrandt, A. C. (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50, 199–204.Google Scholar
  15. 15.
    Nitsch, J. P. and Nitsch, C. (1969) Haploid plants from pollen. Science 163, 85–87.CrossRefGoogle Scholar
  16. 16.
    George, E. F., Puttock, D. J. M., and George, H. J. (1987) Plant Culture Medial, Exegetics, Westbury, UK.Google Scholar
  17. 17.
    Zhu, X. Y. and Negrutiu, I. (1991) Isolation and culture of protoplasts, in A laboratory Guide for Cellular and Molecular Plant Biology, Birkhäuser-Verlag, Basel, Switzerland pp. 18–27.Google Scholar
  18. 18.
    Davies, P. J. (1995) Plant Hormones, Physiology, Biochemistry and Molecular Biology. Kluwer, Dordrecht, The Netherlands.Google Scholar
  19. 19.
    Toriyama, K., Hinata, K., and Sasaki, T. (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor. Appl. Genet. 73, 16–19.CrossRefGoogle Scholar
  20. 20.
    Hagendoorn, M. J. M., Jamar, D. C. L., and van der Plas, L. H. W. (1999) Directing anthraquinone accumulation via manipulation of Morinda suspension cultures, in Plant Cell Culture Protocols, (Hall, R. D., ed), Humana Press, Totowa, NJ, pp. 383–392.CrossRefGoogle Scholar
  21. 21.
    Kao, K. N. and Michayluk, M. R. (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126, 105–110.CrossRefGoogle Scholar
  22. 22.
    Yeoman, M. M. (1973) Tissue (callus) culture techniques, in Plant Cell and Tissue Culture, (Street, H. E., ed.), Blackwell, Oxford, UK, pp. 31–58.Google Scholar
  23. 23.
    de Fossard, R. A. (ed.) (1976) Tissue Culture for Plant Propagators. University of New England Press, Armidale, Australia.Google Scholar

Copyright information

© Humana Press Inc 2000

Authors and Affiliations

  1. 1.BU Cell CybemeticsPlant Research InternationalWageningenThe Netherlands

Personalised recommendations