Molecular Biotechnology

, Volume 13, Issue 3, pp 215–222 | Cite as

Time-resolved fluoroimmunoassay

  • Paolo Degan
  • Adriano Podestà
  • Giorgio Montagnoli


A method is reported to set up a standard competitive TR-FIA. A simple and inexpensive way to prepare reagents and carry out operations is presented as well, with the aim to make it possible to perform a very sensitive analytical procedure in a personalized way within a nondedicated biochemistry laboratory. This protocol is general and can be easily modified with consideration to the analytical target. Once the antibody is available, both its labeling with diethylenetriaminepentaacetic acid dianhydride and Eu3+, and the setting-up of the assay with measurement of europium ion time-resolved fluorescence in a home-made enhancement solution become feasible.

Index Entries

Fluoroimmunoassay, time-resolution europium ion flourescence enhancement 


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Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • Paolo Degan
    • 1
  • Adriano Podestà
    • 2
  • Giorgio Montagnoli
    • 2
  1. 1.National Institute for Research on CancerGenoaItaly
  2. 2.Department of Anatomy, Biochemistry and veterinary PhysiologyUniversity of PisaPisaItaly

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