Journal of Molecular Neuroscience

, Volume 29, Issue 2, pp 123–129 | Cite as

Tpc1808 increases expression of NF-H in PC12 cells

  • Mei Liu
  • Xiaosong Gu
  • Qi Zhang
  • Yan Liu
  • Sheyu Lin
  • Yun Gu
  • Fei Ding
Original Article

Abstract

Tpc1808 is a candidate chemotropic factor induced by nerve injury, and we report here the role of rat Tpc1808 in the expression of high-molecular-weight neurofilament (NF-H) in PC12 cells. A full-length Tpc1808 cDNA encoding the 275-amino-acid protein was constructed into pcDNA-HA and transfected into PC12 cells. The increased expression of NF-H was detectable in PC12-Tpc1808 clones by semi-qRT-PCR, real-time PCR, Western blot, and immunofluorescence. Such an increase could also be observed in PC12 cells subjected to recombinant Tpc1808 protein by real-time PCR and immunohistochemistry. At a concentration of 0.1 μg/mL, Tpc1808 protein, similar to NGF, could promote the expression of NF-H in a time-dependent manner. Our findings suggest that Tpc1808 is the gene related to promotion of nerve growth, and both the Tpc1808 gene and the Tpc1808 recombinant protein up-regulate the expression of NF-H in PC12 cells.

Index Entries

Tpc1808 high-molecular-weight neurofilament PC12 

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Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  • Mei Liu
    • 1
    • 2
  • Xiaosong Gu
    • 1
  • Qi Zhang
    • 1
  • Yan Liu
    • 1
  • Sheyu Lin
    • 1
  • Yun Gu
    • 1
  • Fei Ding
    • 1
  1. 1.The Jiangsu Key Laboratory of NeuroregenerationNantong UniversityNantongPeoples Republic of China
  2. 2.The Institute of Life ScienceJiangsu UniversityZhenjiangPeoples Republic of China

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