Journal of Molecular Neuroscience

, Volume 21, Issue 2, pp 103–110 | Cite as

Increased gene expression of β-1,4-galactosyltransferase I in rat injured sciatic nerve

  • Aiguo Shen
  • Dan Zhu
  • Fei Ding
  • Min Zhu
  • Xiaosong Gu
  • Jianxin Gu
Original Article


During neurite outgrowth on basal lamina, cell-surface β-1,4-galactosyltransferase I (β-1,4-GalT-I) functions as one of the receptors of laminin by binding to N-linked oligosaccharides on the laminin E8 domain. In the present study, it was revealed that in rat injured sciatic nerves, the expression of β-1,4-GalT-I mRNA reached its peak 2–3 d after axotomy in both proximal and distal stumps, and decreased thereafter as demonstrated by Northern blot analysis. In situ hybridization revealed that β-1,4-GalT-I mRNA mainly localized in Schwann cells of the injured nerves. Moreover the Galβ1-4GlcNAc (N-acetylglucosamine) group mainly localized in Schwann cells of the injured nerves by Ricinus communis agglutinin-I (RCA-I) lectin histochemistry. However, the changes in abundance of the Galβ1-4GlcNAc group in injured nerves were not consistent with the expression of β-1, 4-GalT-I mRNA. These findings indicate that β-1,4-GalT-I might be involved in the regeneration of injured peripheral nerves at the early injury stage.

Index Entries

β-1,4-galactosyltransferase I peripheral nerve lesion rat Schwann cell sciatic nerve 


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Copyright information

© Humana Press Inc 2003

Authors and Affiliations

  1. 1.The Jiangsu Province Key Lab of NeuroregenerationNantong Medical CollegeNantongPeople’s Republic of China
  2. 2.Gene Research CenterShanghai Medical College of Fudan University (Former Shanghai Medical University)ShanghaiPeople’s Republic of China

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