Dissecting the humoral immune response to simian immunodeficiency virus
- 55 Downloads
The ultimate goal of an AIDS vaccine is to elicit potent cellular and humoral immune responses that will result in broadly enduring protective immunity. During the past several years, we have focused on characterizing the quantitative and qualitative properties of the antibody response, principally working to define the mechanism(s) of antibody-mediated neutralization in vitro. We have utilized a panel of monoclonal antibodies generated from monkeys infected with attenuated SIV for more than 8 mo to dissect the early events of virus infection involved in antibody-mediated neutralization. Presented herein are highlights from our studies that have identified potential mechanisms by which antibodies neutralize SIV in vitro.
Key WordsNeutralization Antibody Surface plasmon resonance SIV envelope Vaccine
Unable to display preview. Download preview PDF.
- 12.Li A, Katinger H, Posner MR, et al: Synergistic, neutralization of simian-human immunodeficiency virus SHIV-vpu+ by triple and quadruple combinations of human monoclonal antibodies and high-titer anti-human immunodeficiency virus type 1 immunoglobulins. J Virol 1998;72: 3235–3240.PubMedGoogle Scholar
- 33.Kent KA, Bjorling E: B-cell epitopes on the envelope glycoprotiens of SIV and HIV-2; in Korber BTM, Brander C, Moore JP, et al: (eds). HIV Molecular Immunology Database. Theoretical Biology and Biophysics, Los Alamos, NM, 1996, pp 5–21.Google Scholar
- 40.Leonard CK, Spellman MW, Riddle L, Harris RJ, Thomas JN, Gregory TJ: Assigment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotien (gp120) expressed in Chinese hamster ovary cells. J Biol Chem 1990;265: 10373–10382.PubMedGoogle Scholar