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Genetic analysis of CD28 signaling

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Abstract

T cell activation is central to initiating an immune response. Two signals are required: an antigen-specific signal through the T cell receptor (TCR) and an antigen-independent costimulatory signal, primarily through CD28 in naïve T cells. Although many of the molecules involved in TCR signal transduction have been identified, the signaling pathways downstream of CD28 involved in costimulation are not well-defined. Through mutagenesis, we have generated a panel of Jurkat T cell lines in which CD28 costimulation fails to upregulate the RE/AP composite element of the IL-2 promoter. Biochemical analysis and genetic rescue of the defects in these cell lines will lead to a better understanding of CD28 signal transduction.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 288 John Morgan Building 36th Street and Hamilton Walk, 19104, Philadelphia, PA

    Tiffani A. Greene & Virginia Smith Shapiro

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  1. Tiffani A. Greene
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  2. Virginia Smith Shapiro
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Correspondence to Virginia Smith Shapiro.

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Greene, T.A., Shapiro, V.S. Genetic analysis of CD28 signaling. Immunol Res 27, 513–520 (2003). https://doi.org/10.1385/IR:27:2-3:513

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