International Journal of Pancreatology

, Volume 23, Issue 2, pp 137–143 | Cite as

Near-infrared spectrometry analysis of fat, neutral sterols, bile acids, and short-chain fatty acids in the feces of patients with pancreatic maldigestion and malabsorption

  • Teruo Nakamura
  • Tadashi Takeuchi
  • Akinori Terada
  • Yusuke Tando
  • Toshihiro Suda
Article

Summary

Conclusion. Near-infrared spectrometry is a new, rapid, and accurate method for measuring fecal fat that does not require a great deal of chemical knowledge and that can be used by anyone. This method is considered indispensable for the diagnosis of pancreatic steatorrhea and treatment follow-up.

Methods. Fecal fats (GLC method, van de Kamer method), neutral sterols (GLC method), bile acids (GLC method) and short-chain fatty acids (HPLC method) were assayed by the respective conventional methods in 120 subjects, including patients with pancreatic dysfunction, and the results were compared with the those obtained by near-infrared spectrometry. The correlations between fecal fat excretion measured by the GLC method (x) and van de Kamer method (x) and by near-infrared spectrometry (y) were expressed by y=1.10 x-0.16 (r=0.949, P<0.01) and y=0.750x+1.654 (r=0.930, p<0.01), respectively.

Results. The sensitivity and specificity of near-infrared spectrometry for fecal fats were 94.9 and 98.2%, respectively, when compared with the GLC method, and 87.5 and 90.0%, respectively, when compared with the van de Kamer method. In contrast, near-infrared spectrometry was not nearly as accurate as the conventional methods for determining neutral sterols, bile acids, and short-chain fatty acids.

Keywords

Bile Acid Pancreatology Volume Pancreatic Insufficiency Coprostanol Neutral Sterol 

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Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • Teruo Nakamura
    • 2
  • Tadashi Takeuchi
    • 1
  • Akinori Terada
    • 2
  • Yusuke Tando
    • 2
  • Toshihiro Suda
    • 2
  1. 1.Institute of GastroenterologyTokyo Women’s Medical CollegeTokyoJapan
  2. 2.Third Department of Internal MedicineHirosaki University School of MedicinAomoriJapan

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