Endocrine Pathology

, Volume 16, Issue 3, pp 229–238 | Cite as

Expression of heterogeneous nuclear ribonucleoproteins A2 and B1 in the thyroid follicular cells

  • Yukiko Yano
  • WenWen Wu
  • Hiroshi Kamma
  • Masachika Fujiwara
  • Hisato Hara
  • Tohru Yashiro
  • Ei Ueno
  • Masanao Miwa
  • Yuji Aiyoshi
Clinical Research


Several proteins implicated in hormonogenesis of the thyroid have alternatively spliced isoforms. Alternative splicing of pre-mRNA is considered to be important to regulate the hormonal activity. Heterogeneous nuclear ribonucleoproteins (hnRNP) A2 and B1 are two of the abundant nuclear RNA-binding proteins involved in alternative splicing. We examined the expression of hnRNP A2 and B1 in the thyroid, paying particular attention to the relationship between their function and the cellular morphology. B1 was expressed more frequently in cuboidal follicular cells that are hormonally active than in flat follicular cells in normal thyroid, although A2 expression showed no significant difference in two cell types. In Graves’ disease, the patients who had high serum levels of triiodothyronine and thyroxine showed significantly increased expression of B1. B1 expression did not differ significantly between normal thyroids and thyroid neoplasms, except undifferentiated (anaplastic) carcinoma. Conclusively, B1 expression varied in relation to hormonal activity in thyroid follicular cells. B1 protein is a good immunohistological marker to detect hormonal activity of follicular cells, and may provide a key to elucidate the splicing mechanisms involved in thyroid hormonogenesis.

Key Words

Graves’ disease hnRNP A2/B1 hormonal activity thyroid cancer thyroid function 


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Copyright information

© Humana Press Inc 2005

Authors and Affiliations

  • Yukiko Yano
    • 1
  • WenWen Wu
    • 2
  • Hiroshi Kamma
    • 4
  • Masachika Fujiwara
    • 4
  • Hisato Hara
    • 1
  • Tohru Yashiro
    • 1
  • Ei Ueno
    • 1
  • Masanao Miwa
    • 3
  • Yuji Aiyoshi
    • 1
  1. 1.Institute of Clinical MedicineUniversity of TsukubaIbarakiJapan
  2. 2.School of MedicineSt. Marianna UniversityKanagawaJapan
  3. 3.Institute of Basic Medical SciencesUniversity of TsukubaIbarakiJapan
  4. 4.Department of PathologyKyorin University, School of MedicineTokyoJapan

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