Endocrine

, Volume 8, Issue 1, pp 45–50 | Cite as

Concentration of mRNA encoding 3β-hydroxysteroid dehydrogenase/Δ54 isomerase (3β-HSD) and 3β-HSD enzyme activity following treatment of ewes with prostaglandin F

  • Jennifer L. Juengel
  • Bernadette M. Meberg
  • Eric W. McIntush
  • Michael F. Smith
  • Gordon D. Niswender
Article

Abstract

The objectives of these experiments were (1) to determine if prostaglandin F (PGF) decreased mRNA encoding 3β-hydroxysteroid dehydrogenase/d54 isomerase (3β-HSD) specifically in large steroidogenic luteal cells, which contain the high affinity receptors for PGF; and (2) to determine if the decreased concentration of mRNA encoding 3β-HSD following administration of PGF was associated with a decrease in 3β-HSD enzyme activity. Ewes on days 11 or 12 of the estrous cycle were administered PGF (25 mg iv followed by 10 mg im 2 h later) and corpora lutea collected 4, 12, 24, or 48 h later (n = 4–5/time). Corpora lutea were also collected from non-injected (n=4) or saline-injected (n=4) control ewes. Administration of PGF2 decreased (P < 0.05) steady-state concentrations of mRNA encoding 3β-HSD to 35, 15, 9, and 5 percent of the concentrations in the control group at 4, 12, 24, and 48 h, respectively. Concentrations of mRNA encoding 3β-HSD in large luteal cells were decreased to 43% of controls 4 h following injection, which was similar to the decrease seen in steady-state concentrations of this mRNA in total luteal mRNA (35%). However, 3β-HSD enzyme activity was not significantly decreased by 48 h after PGF injection. Thus, the dramatic decreased in mRNA encoding 3β-HSD was not associated with an immediate decrease in 3β-HSD enzyme activity and, therefore, does not appear to be responsible for the acute decrease in secretion of progesterone from ovine luteal tissue during PGF-induced luteolysis.

Key Words

3β-hydroxysteroid dehydrogenase/Δ54 isomerase mRNA enzyme activity corpus luteum ovine 

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Copyright information

© Humana Press Inc 1998

Authors and Affiliations

  • Jennifer L. Juengel
    • 2
  • Bernadette M. Meberg
    • 2
  • Eric W. McIntush
    • 1
  • Michael F. Smith
    • 1
  • Gordon D. Niswender
    • 2
  1. 1.Department of Animal ScienceUniversity of MissouriColumbia
  2. 2.Animal Reproduction and Biotechnology Laboratory, Department of PhysiologyColorado State UniversityFort Collins

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