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Endocrine

, Volume 19, Issue 3, pp 339–344 | Cite as

Castration reduces mRNA levels for calcium regulatory proteins in rat heart

Article

Abstract

Sex-related differences in the cardiac phenotype have been well established. This study was designed to determine whether androgens regulate myocardial gene expression and play a role in the sex-related differences in the myocardial phenotype. Gonadectomized male rats were treated with testosterone, and myocardial gene expression was examined in whole heart using quantitative real-time PCR. Gonadectomy produced a substantial decrease in mRNA levels for the androgen receptor, Na+/Ca2+ exchanger, L-type calcium channel, and β1-adrenergic receptor (β1AR). Supplementation of testosterone in castrates produced a fivefold increase in androgen receptor mRNA levels. Testosterone treatment of castrates produced almost a sixfold increase in Na+/Ca2+ exchanger mRNA, a tenfold increase in L-type calcium channel mRNA accumulation, and a fourfold increase in β1AR mRNA levels. Increased calcium channel expression, β1AR expression, and Na+/Ca2+ exchanger expression together may alter cytosolic calcium. These results provide the first evidence that testoster-one regulates expression of myocardial calcium regulating genes and thus may play a role in modulating the cardiac phenotype in males.

Key Words

Testosterone calcium channel androgen receptor Na+/Ca2+ exchanger β1-adrenergic receptor 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  1. 1.Department of Physiology, and Internal MedicineWayne State UniversityUSA
  2. 2.John D. Dingell VA Medical CenterDetroit

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