Endocrine

, Volume 18, Issue 2, pp 179–184 | Cite as

Direct in vitro effects of androgens and estrogens on ob gene expression and leptin secretion in human adipose tissue

  • Florence Machinal-Quélin
  • Marie-Noëlle Dieudonné
  • René Pecquery
  • Marie-Christine Leneveu
  • Yves Giudicelli
Article

Abstract

In the present study, we have explored, in vitro, the possibility that short exposure to androgens and estrogens for 24 h may directly influence leptin expression (ARNm and secretion) in sc adipose tissue from men and women. In men, only dihydrotestosterone at high concentration (100 nM) induced a reduction in leptin secretion and ob mRNA level. In women, 17β-estradiol (10–100 nM) increased ob mRNA expression (+180 to +500%) and leptin release (+75%). Moreover, in adipose tissue of women, the estrogen precursors testosterone (100 nM) and dehydroepiandrosterone (1 µM) also induced an increase in leptin secretion (+84 and +96%, respectively), an effect that was prevented by the aromatase inhibitor letrozole. Finally, the stimulatory effect of 17β-estradiol observed in women was antagonized by the antiestrogen ICI182780. Altogether, these results suggest that the sexual dimorphism of leptinemia in humans is mainly owing to the estrogen receptor-dependent stimulation of leptin expression in adipose tissue by estrogens and estrogen precursors in women.

Key Words

ob mRNA leptin secretion sex hormones human adipose tissue 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Florence Machinal-Quélin
    • 1
  • Marie-Noëlle Dieudonné
    • 1
  • René Pecquery
    • 1
  • Marie-Christine Leneveu
    • 1
  • Yves Giudicelli
    • 1
  1. 1.Service de Biochimie et de Biologie Moléculaire, Faculté de Médecine Paris-OuestUniversité René Descartes (Paris V), Centre Hospitalier de PoissyPoissy CedexFrance

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