Endocrine

, Volume 18, Issue 1, pp 57–66 | Cite as

Chromosome localization analysis of genes strongly expressed in human visceral adipose tissue

  • Yi-Sheng Yang
  • Huai-Dong Song
  • Wen-Jing Shi
  • Ren-Ming Hu
  • Ze-Guang Han
  • Jia-Lun Chen
Article

Abstract

To understand fully the physiologic functions of visceral adipose tissue and to provide a basis for the identification of novel genes related to obesity and insulin resistance, the gene expression profiling of human visceral adipose tissue was established by using cDNA array. The characterization and chromosome localization of 400 expressed sequence tags (ESTs) strongly expressed in visceral adipose tissue were analyzed by searching PubMed, UniGene, the Human Genome Draft Database, and Location Data Base. Two hundred eighty-nine clones were classified into known genes among the 400 ESTs strongly expressed in the tissue. Among them, <20% have been previously reported to be expressed in adipose tissue. The chromosome localization of 389 ESTs strongly expressed in visceral adipose tissue showed that their relative abundance was significantly increased on chromosomes 1, 16, 19, 20, and 22 compared with the expected distribution of the same number of random genes. The intrachromosome distribution of the genes strongly expressed in visceral adipose tissue was concentrated in certain regions, such as 1p36.2–1p36.3, 6p21.3–6p22.1, 19p13.3 and 19q13.1. Among them, the region of 1p36.2–1p36.3 appeared to be specific for visceral adipose tissue. Interestingly, some genes playing an important role in the pathogenesis of insulin signal transduction and adipocyte differentiation, such as tumor necrosis factor-α and its receptors; CCAAT/enhancer-binding protein-α; and phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85β), were also localized in the concentrated regions, which may provide clues to identifying novel genes closely related to adipocyte function with potential pathophysiologic implications.

Key Words

Visceral adipose cDNA array gene expression chromosome distribution 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Kim, S. and Moustaid-Moussa, N. (2000). J. Nutr. 130, 31,10S-31,15S.Google Scholar
  2. 2.
    Gabrielsson, B. L., Carlsson, B., and Carlsson, L. M. (2000). Obes. Res. 8, 374–384.PubMedGoogle Scholar
  3. 3.
    Montague, C. T. and O’Rahilly, S. (2000). Diabetes 49, 883–888.PubMedCrossRefGoogle Scholar
  4. 4.
    Adams, M. D., Kerlavage, A. R., Fleischmann, R. D., et al. (1995). Nature 377, 3–174.PubMedGoogle Scholar
  5. 5.
    Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., and Friedman, J. M. (1994). Nature 372, 425–432.PubMedCrossRefGoogle Scholar
  6. 6.
    Rajkumar, K., Krsek, M., Dheen, S. T., and Murphy, L. J. (1996). J. Clin. Invest. 98, 1818–1825.PubMedGoogle Scholar
  7. 7.
    Rajkumar, K., Modric, T., and Murphy, L. J. (1999). J. Endocrinol. 162, 457–465.PubMedCrossRefGoogle Scholar
  8. 8.
    Brun, R. P., Kim, J. B., Hu, E., Altiok, S., and Spiegelman, B. M. (1996). Curr. Opin. Cell. Biol. 8, 826–832.PubMedCrossRefGoogle Scholar
  9. 9.
    Bortoluzzi, S., Rampoldi, L., Simionati, B., et al. (1998). Genome Res. 8, 817–825.PubMedGoogle Scholar
  10. 10.
    Deloukas, P., Schuler, G. D., Gyapay, G., et al. (1998). Science 282, 744–746.PubMedCrossRefGoogle Scholar
  11. 11.
    Moller, D. E. (2000). Trends Endocrinol. Metab. 11, 212–217.PubMedCrossRefGoogle Scholar
  12. 12.
    Du, W., Sun, H., Wang, H., et al. (2001). Chin. Med. J. (Engl.) 114, 876–878.Google Scholar
  13. 13.
    Perusse, L., Chagnon, Y. C., Weisnagel, S. J., Rankinen, T., Snyder, E., Sands, J., and Bouchard, C. (2001) Obes. Res. 9, 135–169.PubMedCrossRefGoogle Scholar
  14. 14.
    Hu, R. M., Han, Z. G., Song, H. D., et al. (2000). Proc. Natl. Acad. Sci. USA 97, 9543–9548.PubMedCrossRefGoogle Scholar
  15. 15.
    Mao, M., Fu, G., Wu, J. S., et al. (1998). Proc. Natl. Acad. Sci. USA 1998 95, 8175–8180.CrossRefGoogle Scholar
  16. 16.
    Xu, X. R., Huang, J., Xu, Z. G., et al. (2001). Proc. Natl. Acad. Sci. USA 98, 15,089–15,094.Google Scholar
  17. 17.
    Xu, L., Hui, L., Wang, S., Gong, J., Jin, Y., Wang, Y., Ji, Y., Wu, X., Han, Z., and Hu, G. (2001). Cancer Res. 61, 3176–3181.PubMedGoogle Scholar
  18. 18.
    Collins, A., Frezal, J., Teague, J., and Morton, N. E. (1996). Proc. Natl. Acad. Sci. USA 93, 14,771–14,775.Google Scholar
  19. 19.
    Nelson, B. A., Robinson, K. A., and Buse, M. G. (2000). Diabetes 49, 981–991.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Yi-Sheng Yang
    • 2
  • Huai-Dong Song
    • 2
  • Wen-Jing Shi
    • 2
  • Ren-Ming Hu
    • 2
  • Ze-Guang Han
    • 1
  • Jia-Lun Chen
    • 2
  1. 1.Chinese National Human Genome CenterShanghaiChina
  2. 2.Rui-Jin Hospital, Shanghai Institute of EndocrinologyShanghai Second Medical UniversityShanghaiChina

Personalised recommendations