, Volume 15, Issue 2, pp 187–192 | Cite as

Release of norepinephrine from human ovary

Coupling to steroidogenic response
  • Hernán E. Lara
  • Arnaldo Porcile
  • Jaime Espinoza
  • Carmen Romero
  • Sandra M. Luza
  • Juan Fuhrer
  • Cristian Miranda
  • Luis Roblero


We investigated the possibility that norepinephrine from the human ovary is released after nerve stimulation and that this neurotransmitter is coupled to a steroidogenic response. Biologically significant levels of both norepinephrine and dopamine were found in human ovarian biopsies. [3H]norepinephrine incorporated in vitro was readily released by electrical stimulation in a Ca2+-dependent process. Ovarian membrane preparations exhibited specific binding sites for the β-adrenergic antagonist [3H]dihydroalprenolol. displacement of [3H]dihydroalprenolol with zinterol (a specific β2-agonist, indicated that 72% of these sites were type β2-receptors. β-receptors were also present on granulosa cells. Stimulation of granulosa cells with luteinizing hormone or the β-agonist isoproterenol increased the release of progesterone after 4 d in culture. These results suggest that the sympathetic nerves present in human ovary are coupled to β-adrenergic receptors present in endocrine cells and, as in nonprimate mammals, appear to participate in the regulation of ovarian function.

Key words

Sympathetic nerves β-receptor human ovary 


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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Hernán E. Lara
    • 1
  • Arnaldo Porcile
    • 2
  • Jaime Espinoza
    • 3
  • Carmen Romero
    • 3
  • Sandra M. Luza
    • 1
  • Juan Fuhrer
    • 4
  • Cristian Miranda
    • 3
  • Luis Roblero
    • 5
  1. 1.Laboratorio de Neurobioquímica, Departamento de Bioquimica y Biología Molecular, Facultad de Ciencias Químicas y FarmacéuticasUniversidad de ChileSantiagoChile
  2. 2.Departamento de Obstetricia y Ginecología, Campus Oriente Facultad de MedicinaUniversidad de ChileSantiagoChile
  3. 3.Departamento de Obstetricia y Ginecología, Hospital ClínicoUniversidad de ChileSantiagoChile
  4. 4.Hospital San JosèSantiagoChile
  5. 5.Clínica AlemanaSantiagoChile

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