Endocrine

, Volume 14, Issue 3, pp 303–309 | Cite as

Glycogen accumulation in rat pancreatic islets

In vitro experiments
Article

Abstract

Under conditions of sustained hyperglycemia, glycogen accumulates in pancreatic islets, but not so in acinar pancreatic cells. Advantage conceivably could be taken from such a situation in the perspective of the noninvasive imaging of the endocrine pancreas. The present experiments aim, therefore, at characterizing the time course for glycogen accumulation in pancreatic islets cultured at a high concentration (30 mM) of d-glucose in the presence of tracer amounts of either d-[U-14C]glucose or 2-deoxy-2 [18F]fluoro-d-glucose. The 14C-labeled glycogen content of the cultured islets increased with time (150 min to 72 h), exceeded that found in acinar tumoral cells, and did not decrease over 60 min of incubation at 30 mM d-glucose in the absence of d-[U-14C]glucose. Glycogenolysis was observed, however, when the concentration of d-glucose was decreased to 2.8 mM and, in such a case, was further enhanced by forskolin and theophylline. Such a glycogenolysis concided with the generation of 14CO2 from radioactive intracellular precursors and alteration of the B-cell secretory response to d-glucose. The radioactive glycogen content was higher in islets exposed to 2-deoxy-2-[18F]fluoro-d-glucose than d-[U-14C]glucose. Prior exposure of the islets to streptozotocin suppressed the accumulation of glycogen during their subsequent culture at high d-glucose concention. These findings may help to define the experimental conditions optimal for the labeling and accumulation of islet glycogen in vivo.

Key Words

Glycogen pancreatic islets endocrine pancreas imaging 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Malaisse, W. J., Malaisse-Lagae, F., and Mayhew, D. A. (1967). J. Clin. Invest. 46, 1724–1734.PubMedGoogle Scholar
  2. 2.
    Malaisse, W. J., Sener, A., Koser, M., and Malaisse-Lagae, F. (1977). Biochem. J. 164, 447–454.PubMedGoogle Scholar
  3. 3.
    Marynissen, G., Leclercq-Meyer, V., Sener, A., and Malaisse, W. J. (1990). Metabolism 39, 87–95.PubMedCrossRefGoogle Scholar
  4. 4.
    Malaisse, W. J., Maggetto, C., Leclercq-Meyer, V., and Sener, A. (1993). J. Clin. Invest. 91, 432–436.PubMedGoogle Scholar
  5. 5.
    Like, A. A., Gerritsen, G. C., Dulin, W. E., and Gaudreau, P. (1974). Diabetologia 10, 509–520.PubMedCrossRefGoogle Scholar
  6. 6.
    Brisson, G. R., Malaisse-Lagae, F., and Malaisse, W. J. (1972). J. Clin. Invest. 51, 232–241.PubMedCrossRefGoogle Scholar
  7. 7.
    Orci, L., Gabbay, K. H., and Malaisse, W. J. (1972). Science 175, 1128–1130.PubMedCrossRefGoogle Scholar
  8. 8.
    Sener, A., Malaisse-Lagae, F., and Malaisse, W. J. (1993). Biochim. Biophys. Acta 1177, 54–60.PubMedCrossRefGoogle Scholar
  9. 9.
    Golden, P., Baird, L., Malaisse, W. J., Malaisse-Lagae, F., and Walker, M. O. (1971). Diabetes 20, 513–518.PubMedGoogle Scholar
  10. 10.
    Christophe, J. (1994). Am. J. Physiol. 266, G963-G971.PubMedGoogle Scholar
  11. 11.
    Zhang, T.-M., Maggetto, C., and Malaisse, W. J. (1994). Biochem. Med. Metab. Biol. 51, 129–139.PubMedCrossRefGoogle Scholar
  12. 12.
    McSheehy, P. M. J., Leach, M. O., Judson, I. R., and Griffiths, J. R. (2000). Cancer Res. 60, 2122–2127.PubMedGoogle Scholar
  13. 13.
    Hamacher, K., Coenen, H. H., and Stocklin, G. (1986). J. Nucl. Med. 27, 235–238.PubMedGoogle Scholar
  14. 14.
    Lemaire, C., Damhaut, P., Lauricella, B., et al. (1997). J. Labet Compound Radiopharm. 40, 256,257.Google Scholar
  15. 15.
    Camara, J., Valverde, I., and Malaisse, W. J. (1990). Med. Sci. Res. 18, 61,62.Google Scholar
  16. 16.
    Malaisse-Lagae, F. and Malaisse, W. J. (1984). In: Methods in diabetes research. Larner, J. and Pohl, S. L. (eds.). Wiley. New York.Google Scholar
  17. 17.
    Malaisse, W. J., Giroix, M.-H., Malaisse-Lagae, F., and Sener, A. (1986). Am. J. Physiol. 251, C841-C846.PubMedGoogle Scholar
  18. 18.
    Hutton, J. C., Sener, A., and Malaisse, W. J. (1979). Biochem. J. 184, 291–301.PubMedGoogle Scholar
  19. 19.
    Malaisse, W. J. and Sener, A. (1988). Biochim. Biophys. Acta 971, 246–254.PubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  1. 1.Laboratory of Experimental MedicineBrussels Free UniversityBrusselsBelgium

Personalised recommendations