Abstract
The present studies revealed that hepatocyte growth factor (HGF) disrupts cell contact, increases both type 3 IP3 receptor and intracellular calcium ([Ca2+) levels and induces apoptosis of rat ovarian surface epithelial cells (ROSE-179 cells). Type 3 IP3 receptor was only increased in cells that lost cell contact. Disrupting cell contact by depleting extracellular calcium (Ca2+) also resulted in an increase in [Ca2+]i levels and an increase in apoptosis. These responses were prevented by the addition of 0.7 mM Ca2+. Actinomycin D and cyclo-heximide prevented apoptosis that resulted from Ca2+ removal. In situ hybridization studies revealed that type 3 IP3 receptor was expressed at relatively low levels by ROSE-179 cells cultured with Ca2+ but at high levels in the absence of Ca2+. ROSE-179 cells cultured in Ca2+-free medium with type 3 IP3 receptor antisense oligonucleotide lost cell contact but did not show an increase in either type 3 IP3 receptor protein, [Ca2+]i, or apoptosis. The nonsense oligonucleotide did not alter these responses to Ca2+ removal. Thus, the disruption of cell contact by either HGF or Ca2+ depletion increases the expression of type 3 IP3 receptor, which causes an increase in [Ca2+]i and the apoptotic death of ROSE-179 cells.
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Kiess, W. and Gallaher, B. (1998). Eur. J. Endocrinol. 138, 482–491.
Chun, S. Y., Eisenhauer, K. M., Minami, S., and Hsueh, A. J. (1996). Semin. Reprod. Endocrinol. 14, 197–02.
Chun, S. Y., Eisenhauer, K. M., Minami, S., Billig, H., Perlas, E., and Hsueh, A. J. (1996). Endocrinology 137, 1447–1456.
Boudreau, N., Werb, Z., and Bissell, M. J. (1996). Proc. Natl. Acad. Sci. USA 93, 350–3513.
Newby, A. C. and George, S. J. (1996). Curr. Opin. Cardiol. 11, 574–582.
Werb, Z., Sympson, C. J., Alexander, C. M., Thomasset, N., Lund, L. R., MacAuley, A., Ashkenas, J., and Bissell, M. J. (1996). Kidney Int. 54(Suppl.), S68-S74.
Peluso, J. J. (1997). Biochem. Pharmacol. 54, 847–853.
Hess, S., Gulati, R., and Peluso, J. J. (1999). Endocrinology 140, 2908–2916.
Peluso, J. J., Pappalardo, A., and Trolice, M. P. (1996). Endocrinology 137, 1196–1203.
Trolice, M. P., Pappalardo, A., and Peluso, J. J. (1997). Endocrinology 138, 107–113.
Aoshiba, K., Rennard, S. I., and Spurzem, J. R. (1997). Am. J. Physiol. 272, L28-L37.
Lagneaux, L., Delforge, A., Bron, D., De Bruyn, C., and Stryckmans, P. (1998). Blood 91, 2387–2396.
Hynes, R. O. (1992). Cell 69, 11–25.
Zondag, G. C. M. and Moolenaar, W. H. (1997). Biochimie 79, 477–483.
Pignatelli, M. (1998). J. Pathol. 186, 1–2.
Gulati, R. and Peluso, J. J. (1997). Endocrinology 138, 1847–1856.
Marks, A. R. (1997). Am. J. Physiol. 272 H597-H605.
Mikoshiba, K. (1997). Curr. Opin. Neurobiol. 7, 339–345.
Parys, J. B., De Smedt, H., Missiaen, L., Bootman, M. D., Sienaert, I., and Casteels, R. (1995). Cell Calcium 17, 239–249.
Fissore, R. A., Longo, F. J., Anderson, E., Parys, J. B., and Ducibella, T. (1999). Biol. Reprod. 60, 49–57.
Fujino, I., Yamada, N., Miyawaki, A., Hasegawa, M., Furuichi, T., and Mikoshiba K. (1995). Cell Tissue Res. 280, 201–210.
Furuichi, T., Shiota, C., and Mikoshiba, K. (1990). FEBS Lett. 267, 85–88.
Khan, A. A., Soloski, M. J., Sharp, A. H., Schilling, G., Sabatini, D. M., Li, S. H., Ross, C. A., Snyder, S. H. (1996). Science 273, 503–507.
Sugawara, H., Kurosaki, M., Takata, M., and Kurosaki, T. (1997). EMBO J 16, 3078–3088.
Darzynkiewicz, Z., Juan, G., Li, X., Gorczyca, W., Murakami, T., and Traganos, F. (1997). Cytometry 27, 1–20.
Gutstein, D. E. and Marks, A. R. (1997). Heart Vessels 12, 53–57.
DeLisle, S., Blondel, O., Longo, F. J., Schnabel, W. E., Bell, G. I., and Welsh, M. J. (1996). Am. J. Physiol. 270, C1255-C1261.
Shibamoto, S., Hayakawa, M., Takeuchi, K., Hori, T., Oku, N., Miyazawa, K., Kitamura, N., Takeichi, M., and Ito, F. (1994). Cell Adhesion Commun. 1, 295–305.
Tannapfel, A., Yasui, W., Yokozaki, H., Wittekind, C., and Tahara, E. (1994). Virchows Arch. 425, 139–144.
Gumbiner, B. M. (1993). Neuron 11, 551–564.
Gatti, R., Belletti, S., Orlandini, G., Bussolati, O., Dall'Asta, V., and Gazzola, G. C. (1998). J. Histochem. Cytochem. 46, 895–900.
Del Bino, G., Darzynkiewicz, Z., Degraef, C., Mosselmans, R., Fokan, D., and Galand, P. (1999). Cell Prolif. 32, 25–37.
Waldron, R. T., Short, A. D., Meadows, J. J., Ghosh, T. K., and Gill, D. L. (1994). J. Biol. Chem. 269, 11,927–11,933.
Chiesa, R., Angeretti, N., Del Bo, R., Lucca, E., Munna, E., and Forloni, G. (1998). J. Neurochem. 70, 1474–1483.
White, B. A. and Bancroft, C. (1987). Methods Enzymol. 139, 655–667.
Delidow, B. C., Lail-Trecker, M., and White, B. A. (1992). Mol. Endocrinol. 6, 1268–1276.
Luciano, A. M., Pappalardo, A., Ray, C., and Peluso, J. J. (1994). Biol. Reprod. 51, 646–654.
De Smedt, H., Missiaen, L., Parys, J. B., Bootman, M. D., Mertens, L., Van Den Bosch, L., and Casteels, R. (1994). J. Biol. Chem. 269, 21,691–21,698.
Blondel, O., Takeda, J., Janssen, H., Seino, S., and Bell, G. I. (1993). J. Biol. Chem. 268, 11,356–11,363.
Nash, D. M., Hess, S. A., White, B. A., and Peluso, J. J. (1998). Endocrinology 139, 4663–4671.
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Lail-Trecker, M.R., Peluso, C.E. & Peluso, J.J. Hepatocyte growth factor disrupts cell contact and stimulates an increase in type 3 inositol triphosphate receptor expression, intracellular calcium levels, and apoptosis of rat ovarian surface epithelial cells. Endocr 12, 303–314 (2000). https://doi.org/10.1385/ENDO:12:3:303
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DOI: https://doi.org/10.1385/ENDO:12:3:303