, Volume 10, Issue 2, pp 105–111 | Cite as

Ovine prostaglandin F receptor

Steroid influence on steady-state levels of luteal mRNA
  • Patricia B. Hoyer
  • Samuel L. Marion
  • Ian Stine
  • Bo R. Rueda
  • Debora L. Hamernik
  • John W. Regan
  • Mark E. Wise


Expression of the receptor for prostaglandin F (PGF) is decreased in the ovine corpus luteum during regression and increased in early pregnancy. This study was designed to evaluate the influence of progesterone and/or 17β-estradiol (E2) on this regulation. Circulating progesterone (functional regression) and luteal PGF receptor mRNA decreased (p<0.05) within 8 h of PGF-induced luteal regression in midluteal phase (day 10; d 10) ewes; however, internucleosomal DNA fragmentation (structural regression) was not yet increased. Additionally, luteal PGF receptor mRNA and circulating progesterone were greater (p<0.05) in pregnant than in nonpregnant ewes on d 14, but not on d 12. Twelve hours following injection of d 10 ewes with E2, steady-state levels of mRNA for PGF receptor were decreased (p<0.05), although circulating progesterone and DNA laddering were unchanged. Conversely, luteal mRNA for PGF receptor was increased (p<0.05) by E2 treatment in hysterectomized ewes. These results provide evidence that (1) luteal PGF receptor expression parallels circulating progesterone levels during functional regression and in early pregnancy, but (2) expression of PGF receptor can be dissociated from alterations in circulating progesterone by injection with E2. Additionally, decreased PGF receptor expression initiated by E2 is uterine-dependent, whereas the direct luteal effect (hysterectomized ewes) of E2 is a stimulation of PGF receptor expression. These results collectively support the belief that the apparent downregulation of PGF receptor during luteal regression is associated with uterine-derived PGF and its intracellular effects rather than with alterations in ovarian steroid production.

Key Words

Ovine corpus luteum FP luteal regression progesterone 17β-estradiol 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Hauger, R. L., Karsch, F. J., and Foster, D. L. (1977). Endocrinology 101, 807–817.PubMedCrossRefGoogle Scholar
  2. 2.
    McCracken, J. A., Glew, M. E., and Scaramuzzi, R. J. (1970). J. Clin. Endocrinol. Metab. 30, 544–546.PubMedCrossRefGoogle Scholar
  3. 3.
    McCracken, J. A., Carlson, J. C., Glew, M. E., Goding, J. R., Baird, D. T., Green, K., et al. (1972). Nature New Biol. 83, 527–536.Google Scholar
  4. 4.
    Del Campo, C. H. and Ginther, O. J. (1973). Am. J. Vet. Res. 34, 300–316.Google Scholar
  5. 5.
    Heap, R. B., Fleet, I. R., and Hamon, M. (1985). J. Reprod. Fertil. 74, 645–656.PubMedCrossRefGoogle Scholar
  6. 6.
    Nett, T. M. and Niswender, G. D. (1981). Acta Vet. Scand. 77(Suppl), 117–130.Google Scholar
  7. 7.
    Braden, T. D., Gamboni, F., and Niswender, G. D. (1988). Biol. Reprod. 39, 245–253.PubMedCrossRefGoogle Scholar
  8. 8.
    Rueda, B. R., Wegner, J. A., Marion, S. L., Wahlen, D. D., and Hoyer, P. B. (1995). Biol. Reprod. 52, 305–312.PubMedCrossRefGoogle Scholar
  9. 9.
    McGuire, W. J., Juengel, J. L., and Niswender G. D. (1994). Biol. Reprod. 51, 800–806.PubMedCrossRefGoogle Scholar
  10. 10.
    Fraser, H. M., Abbott, M., Laird, N. C., McNeilly, A. S., Nestor, J. J., Jr., and Vickery, B. H. (1985). J. Endocrinol. 111, 83–90.Google Scholar
  11. 11.
    Pate, J. L. and Nephew, K. P. (1988). Biol. Reprod. 38, 568–576.PubMedCrossRefGoogle Scholar
  12. 12.
    Juengel, J. L., Gaverick, H. A., Johnson, A. L., Youngquist, R. S., and Smith, M. F. (1993). Endocrinology 132, 249–254.PubMedCrossRefGoogle Scholar
  13. 13.
    Hoyer, P. B. (1998). J. Soc. Gynecol. Invest. 5, 49–57.CrossRefGoogle Scholar
  14. 14.
    Balapure, A. K., Caicedo, I. C., Kawada, K., Watt, D. S., Rexroad, C. E., Jr., and Fitz, T. A. (1989). Biol. Reprod. 41, 385–392.PubMedCrossRefGoogle Scholar
  15. 15.
    Wiepz, G. J., Wiltbank, M. C., Nett, T. M., and Niswender, G. D. (1992). Biol. Reprod. 47, 984–991.PubMedCrossRefGoogle Scholar
  16. 16.
    Graves, P. E., Bailey, T. J., Pierce, K. L., Rueda, B. R., Gil, D. W., Woodard, D. F., et al. (1995). Endocrinology 136, 3430–3436.PubMedCrossRefGoogle Scholar
  17. 17.
    Rueda, B. R., Botros, I. W., Pierce, K. L., Regan, J. W., and Hoyer, P. B. (1995). Endocrine 3, 781–787.CrossRefGoogle Scholar
  18. 18.
    Stormshak, F., Kelley, H. E., and Hawk, H. W. (1969). J. Anim. Sci. 29, 476–478.PubMedGoogle Scholar
  19. 19.
    Karsch, F. J., Noveroske, J. W., Roche, J. F., Norton, H. W., and Nalbandov, A. V. (1970). Endocrinology 87, 1228–1236.PubMedGoogle Scholar
  20. 20.
    Hixon, J. E. and Flint, A. P. F. (1987). J. Reprod. Fertil. 79, 457–467.PubMedCrossRefGoogle Scholar
  21. 21.
    Zhang, J., Weston, P. G., and Hixon, J. E. (1991). Biol. Reprod. 45, 395–402.PubMedCrossRefGoogle Scholar
  22. 22.
    Juengel, J. L., Wiltbank, M. C., Meberg, B. M., and Niswender, G. D. (1996). Biol. Reprod. 54, 1096–1102.PubMedCrossRefGoogle Scholar
  23. 23.
    Mamluk, R., Chen, D., Greber, Y., Davis, J. S., and Meidan, R. (1998). Biol. Reprod., 58, 849–856.PubMedCrossRefGoogle Scholar
  24. 24.
    Juengel, J. L., Melner, M. H., Clapper, J. A., Turzillo, A. M., Moss, G. E., Nett, T. M., et al. (1998). J. Reprod. Fertil. 113, 299–305.PubMedCrossRefGoogle Scholar
  25. 25.
    Rothchild, I. (1981). Rec. Prog. Horm. Res. 37, 183–298.PubMedGoogle Scholar
  26. 26.
    Duffy, D. M., Hess, D. L., and Stouffer, R. L. (1994). J. Clin. Endocrinol. Met. 79, 1587–1594.CrossRefGoogle Scholar
  27. 27.
    Spencer, T. E., Becker, W. C., George, P., Mirando, M. A., Ogle, T. F., and Bazer, F. W. (1995). Endocrinology 136, 4932–4944.PubMedCrossRefGoogle Scholar
  28. 28.
    Mirando, M. A., Becker, W. C., and Whiteaker, S. S. (1993). Biol. Reprod. 48, 874–882.PubMedCrossRefGoogle Scholar
  29. 29.
    Glass, J. D., Fitz, T. A., and Niswender, G. D. (1984). Biol. Reprod. 31, 967–974.PubMedCrossRefGoogle Scholar
  30. 30.
    Wegner, J. A., Martinez-Zaguilan, R., Gillies, R. J., and Hoyer P. B. (1991). Endocrinology 128, 929–936.PubMedGoogle Scholar
  31. 31.
    Wiltbank, M. C., Knickerbocker, J. J., and Niswender, G. D. (1989). Biol. Reprod. 40, 1194–1200.PubMedCrossRefGoogle Scholar
  32. 32.
    Hoyer, P. B. and Marion, S. L. (1989). J. Reprod. Fertil. 86, 445–455.PubMedCrossRefGoogle Scholar
  33. 33.
    Bolt, D. J. and Hawk, H. W. (1975). J. Anim. Sci. 40, 687–690.PubMedGoogle Scholar
  34. 34.
    Caraty, A., Locatelli, A., and Martin, G. B. (1989). J. Endocrinol. 123, 375–382.PubMedCrossRefGoogle Scholar
  35. 35.
    Hawk, H. W. and Bolt, D. J. (1970). Biol. Reprod. 2, 275–278.PubMedCrossRefGoogle Scholar
  36. 36.
    Tsai, S., Anderson, L. E., Juengel, J., Niswender, G. D., and Wiltbank, M. C. (1998). J. Reprod. Fertil. 114, 69–75.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1999

Authors and Affiliations

  • Patricia B. Hoyer
    • 1
  • Samuel L. Marion
    • 1
  • Ian Stine
    • 1
  • Bo R. Rueda
    • 4
  • Debora L. Hamernik
    • 1
  • John W. Regan
    • 2
  • Mark E. Wise
    • 3
  1. 1.Department of Physiologythe University of ArizonaTucson
  2. 2.Department of Pharmacology and Toxicologythe University of ArizonaTucson
  3. 3.Department of Animal Sciencesthe University of ArizonaTucson
  4. 4.The Women’s Research Institute, Department of Obstetrics and GynecologyUniversity of Kansas School of MedicineWichita

Personalised recommendations