Endocrine

, Volume 10, Issue 1, pp 7–12 | Cite as

The role of arachidonic acid on LH-stimulated steroidogenesis and steroidogenic acute regulatory protein accumulation in MA-10 mouse leydig tumor cells

  • Xingjia Wang
  • Lance P. Walsh
  • Douglas M. Stocco
Article

Abstract

Metabolic pathways leading to the production of arachidonic acid (AA) and its metabolites have been reported to have modulatory effects on steroidogenesis in a number of cell types. To examine the importance of the arachidonic acid pathway in steroid production and steroidogenic acute regulatory (StAR) protein expression, luteinizing hormones (LH) or N 6-2-o-dibutyryl-adenosine-3∶5-cyclic monophosphate-(Bt2cAMP) stimulated MA-10 mouse Leydig tumor cells were treated with various concentrations of quinacrine (an inhibitor of arachidonic acid production). Incubation of the cells with quinacrine resulted in dose-dependent decreases in steroid production and StAR protein. Twenty micromolars quinacrine inhibited 92 and 91% of LH-induced progesterone and StAR protein, respectively, and 98 and 90% of Bt2cAMP-induced progesterone and StAR protein. Reversal of this inhibition was obtained by incubation of quinacrine-treated cells with various levels of AA, which resulted in a dose-dependent increase in both steroid and StAR protein levels. Two hundred micromolars of AA rescued 57 and 60% of the LH-induced steroid production and StAR protein, respectively, and 52 and 89% of Bt2cAMP-induced steroid production and StAR protein. These results suggest that the effect of AA on LH- and cAMP-stimulated steroidogenesis is associated with the modulation of StAR protein expression.

Key Words

Arachidonic acid quinacrine steroidogenic acute regulatory protein StAR steroid biosynthesis 

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Xingjia Wang
    • 1
  • Lance P. Walsh
    • 1
  • Douglas M. Stocco
    • 1
  1. 1.Department of Cell Biology and BiochemistryTexas Tech University Health Sciences CenterLubbock

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