Skip to main content
Log in

Bone calcium turnover, formation, and resorption in bromocriptine- and prolactin-treated lactating rats

  • Published:
Endocrine Aims and scope Submit manuscript

Abstract

To evaluate the effect of endogenous prolactin (PRL) on bone metabolism, we studied bone calcium turnover by the 45Ca kinetic method and bone formation and resorption by bone histomorphometry and biochemical markers in 13-wk-old lactating Wistar rats. For 1 wk, the animals received daily administration of 0.9% NaCl (control) intraperitoneally, 6 mg of bromocriptine/kg of body wt intraperitoneally, or 6 mg of bromocriptine/kg of body wt plus 2.5 mg of ovine PRL/kg of body wt subcutaneously. Bromocriptine, a dopaminergic inhibitor of endogenous PRL secretion, significantly decreased calcium ion deposit rate and calcium resorption rate in femur, tibia, vertebrae 5 and 6, and sternum by 20–42%. By contrast, calcium resorption rate of the vertebrae and the sternum of the PRL-treated group was higher than that of controls, whereas the tibia and sternum exhibited a greater net loss of calcium. The suppression of bone calcium turnover in the bromocriptine-treated group was further supported by a significant decrease in the urinary deoxypyridinoline, a biochemical index of bone resorption, and the histomorphometric data, which showed changes indicative of suppressed bone resorption and formation. The histomorphometric data from the PRL-treated group were not different from those of the control group with the exception of an increase in the longitudinal growth rate. The results suggested a role of endogenous PRL in the stimulation of bone turnover during lactation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Simpson, A. A., Simpson, M. H. W., Sinha, Y. N., and Schmidt, G. H. (1973). J. Endocrinol. 58, 675, 676.

    Article  PubMed  CAS  Google Scholar 

  2. Toverud, S. U., Harper, C., and Munson, P. L. (1976). Endocrinology (Balt.) 99, 371–378.

    CAS  Google Scholar 

  3. Miller, S. C., Shuppe, J. G., Redd, E. H., Miller, M. A., and Omura, T. H. (1986). Bone 7, 283–287.

    Article  PubMed  CAS  Google Scholar 

  4. Affinito, P., Tommaselli, G. A., Di Carlo, C., Guida, F., and Noppi, C. (1996). J. Clin. Endocrinol. Metab. 81, 2314–2318.

    Article  PubMed  CAS  Google Scholar 

  5. Lamke, B., Brundin, J., and Moberg, P. (1977). Acta Obstet. Gynecol. Scand. 56, 217–219.

    PubMed  CAS  Google Scholar 

  6. Sowers, M. (1996). J. Bone Miner. Res. 11, 1052–1060.

    PubMed  CAS  Google Scholar 

  7. Delmas, P. D. (1993). Osteoporos. Int. 3(Suppl. 1), 81–86.

    Article  PubMed  Google Scholar 

  8. Pike, J. W., Parker, J. B., Haussler, M. R., Boass, A., and Toverud, S. U. (1979). Science 204, 1427–1429.

    Article  PubMed  CAS  Google Scholar 

  9. Toverud, S. U., Boass, M., Haussler, M. R., and Pike, J. W. (1983). J. Steroid Biochem. 19, 505–510.

    Article  PubMed  CAS  Google Scholar 

  10. Halloran, B. P., Barthel, E. N., and DeLuca, H. F. (1979). Proc. Natl. Acad. Sci. USA 17, 5549–5553.

    Article  Google Scholar 

  11. Halloran, B. P. and DeLuca, H. F. (1980). Am. J. Physiol. 239, E64-E68.

    PubMed  CAS  Google Scholar 

  12. Boass, M., Toverud, S. U., Pike, S. W., and Haussler, M. R. (1981). Endocrinology (Balt.) 109, 900–907.

    CAS  Google Scholar 

  13. Amenomori, Y., Chen, C. L., and Meites, J. (1970). Endocrinology (Balt.) 86, 506–510.

    CAS  Google Scholar 

  14. Mainoya, J. R. (1972). Am. Zool. 12, 112 (abstract).

    Google Scholar 

  15. Mainoya, J. R. (1978). Experientia 34, 1230, 1231.

    Article  PubMed  CAS  Google Scholar 

  16. Krishnamra, N., Thumchai, R., and Limlomwongse, L. (1990). Bone Miner. 11, 31–41.

    Article  PubMed  CAS  Google Scholar 

  17. Krishnamra, N., Lotinun, S., and Limlomwongse, L. (1993). Bone Miner. 23, 253–266.

    PubMed  CAS  Google Scholar 

  18. Krishnamra, N. and Taweerathitam, P. (1995). Can. J. Physiol. Pharmacol. 73, 1185–1189.

    PubMed  CAS  Google Scholar 

  19. Lotinun, S., Limlomwongse, L., and Krishnamra, N. (1998). Can. J. Physiol. Pharmacol. 76, 218–228.

    Article  PubMed  CAS  Google Scholar 

  20. Piyabhan, P., Krishnamra, N., and Limlomwongse, L. (2000). Can. J. Physiol. Pharmacol. 78, 757–765.

    Article  PubMed  CAS  Google Scholar 

  21. Krishnamra, N. and Cheeveewattana, V. (1994). Can. J. Physiol. Pharmacol. 72, 1521–1527.

    PubMed  CAS  Google Scholar 

  22. Krishnamra, N. and Seemoung, J. (1996). Can. J. Physiol. Pharmacol. 74, 1157–1165.

    Article  PubMed  CAS  Google Scholar 

  23. Bromage, R. (1989). J. Nutr. 119, 428–438.

    Google Scholar 

  24. Bromage, R. and DeLuca, H. F. (1985). Am. J. Physiol. 248, E182-E187.

    Google Scholar 

  25. Garner, S. C., Boass, A., and Toverud, S. U. (1990). J. Bone Miner. Res. 5, 69–75.

    PubMed  CAS  Google Scholar 

  26. Hodnett, D. W., DeLuca, H. F., and Jorgensen, N. A. (1992). Am. J. Physiol. 262, E230-E233.

    PubMed  CAS  Google Scholar 

  27. Krishnamra, N., Seemoung, J., and Limlomwongse, L. (1997). Endocr. J. 44, 257–264.

    PubMed  CAS  Google Scholar 

  28. Amenomori, Y., Chen, C. L., and Meites, J. (1970). Endocrinology 86, 506–510.

    Article  PubMed  CAS  Google Scholar 

  29. Vance, M. L., Evans, W. S., and Thorner, M. O. (1984). Ann. Intern. Med. 100, 78–91.

    PubMed  CAS  Google Scholar 

  30. Schlechte, J., Walkner, L., and Kathol, M. A. (1992). J. Clin. Endocrinol. Metab. 75, 698–703.

    Article  PubMed  CAS  Google Scholar 

  31. Clement-Lacroix, P., Ormandy, C., Lepescheux, L., et al. (1999). Endocrinology 140, 96–105.

    Article  PubMed  CAS  Google Scholar 

  32. Bataille-Simoneau, N., Gerland, K., Chappard, D., Basle, M. F., and Mercier, L. (1996). Biochem. Biophys. Res. Commun. 229, 323–328.

    Article  PubMed  CAS  Google Scholar 

  33. Tzeng, S. J. and Linzer, D. I. (1997). Mol. Reprod. Dev. 48, 45–52.

    Article  PubMed  CAS  Google Scholar 

  34. Li, X. Q. and Klein, L. (1990). Proc. Exp. Biol. Med. 195, 350–355.

    CAS  Google Scholar 

  35. Kimmel, D. B. and Lee, W. S. S. (1982). Anat. Rec. 203, 31–45.

    Article  PubMed  CAS  Google Scholar 

  36. Podenphant, J. and Engel, U. (1987). Calcif. Tissue Int. 40, 184–188.

    Article  PubMed  CAS  Google Scholar 

  37. Dequecker, J., Remans, J., Franssen, R., and Waes, J. (1971). Calcif. Tissue Res. 7, 23–30.

    Article  Google Scholar 

  38. Doyle, F. (1972). Clin. Endocrinol. Metab. 1, 143–167.

    Article  Google Scholar 

  39. Lauffenburger, T., Olah, A. J., Dambacher, A., Guncaga, J., Lentner, C., and Haas, H. G. (1977). Metabolism 26, 589–605.

    Article  PubMed  CAS  Google Scholar 

  40. Charles, P., Poser, J. W., Mosekilde, L., and Jensen, F. T. (1985). J. Clin. Invest. 76, 2254–2258.

    Article  PubMed  CAS  Google Scholar 

  41. Tamage, R. V. and Grubb, S. A. (1977). Clin. Orthop. Rel. Res. 122, 299–306.

    Google Scholar 

  42. Yoshida, S., Yamamuro, T., Okumura, H., and Takahashi, H. (1991). Bone 92, 185–194.

    Article  Google Scholar 

  43. Dempster, D. W., Birchman, R., Xu, R., Lindsay, R., and Shen, V. (1995). Bone 16, 157–161.

    Article  PubMed  CAS  Google Scholar 

  44. Lees, C. J. and Jerome, C. P. (1998). Bone 22(5), 545–549.

    Article  PubMed  CAS  Google Scholar 

  45. Kolthoff, N., Eithen, P., Kristensen, B., and Nielsen, S. P. (1998). Clin. Sci. 94, 405–412.

    PubMed  CAS  Google Scholar 

  46. Fukuda, S. and Iida, H. (1993). J. Vet. Med. Sci. 55, 565–569.

    PubMed  CAS  Google Scholar 

  47. Budayr, A. A., Halloran, B. P., King, J. C., Diep, D., Nissenson, R. A., and Strewler, G. J. (1989). Proc. Natl. Acad. Sci. USA 86, 7183–7185.

    Article  PubMed  CAS  Google Scholar 

  48. Khosla, S., Johansen, K. L., Ory, S. J., O’Brien, P. C., and Kao, P. C. (1990). Mayo Clin. Proc. 65, 1408–1414.

    PubMed  CAS  Google Scholar 

  49. Sowers, M. F., Hollis, B. W., Shapiro, B., et al. (1996). JAMA 276(7), 549–554.

    Article  PubMed  CAS  Google Scholar 

  50. Dobnig, H., Kainer, F., Stepan, V., et al. (1995). J. Clin. Endocrinol. Metab. 80, 3699–3707.

    Article  PubMed  CAS  Google Scholar 

  51. Henry, J. G., Mitnick, M., Dann, P. R., and Stewart, A. F. (1977). J. Clin. Endocrinol. Metab. 82, 900–906.

    Article  Google Scholar 

  52. Li, X. O. and Klein, L. (1990). Calcif. Tissue Int. 46, 282–283.

    Article  PubMed  CAS  Google Scholar 

  53. Chow, J. W. M., Jagger, C. J., and Chamber, T. J. (1993). Am. J. Physiol. 265(28), E340-E347.

    PubMed  CAS  Google Scholar 

  54. Parfitt, A. M., Drezner, M. K., Glorieux, F. H., et al. (1987). J. Bone Miner. Res. 2(6), 595–610.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nateetip Krishnamra.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lotinun, S., Limlomwongse, L., Sirikulchayanonta, V. et al. Bone calcium turnover, formation, and resorption in bromocriptine- and prolactin-treated lactating rats. Endocr 20, 163–170 (2003). https://doi.org/10.1385/ENDO:20:1-2:163

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1385/ENDO:20:1-2:163

Key Words

Navigation