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Cardiovascular Toxicology

, Volume 4, Issue 4, pp 317–325 | Cite as

Dilated cardiomyopathy in retrovirally infected mice

A novel model for silent viral DCM?
  • Julie Beischel
  • Douglas F. Larson
  • Qianli Yu
  • Bo Yang
  • Ramon Tomas Sepúlveda
  • Tara Kelley
  • Ronald R. Watson
Original Research

Abstract

Dilated cardiomyopathy (DCM) is a clinically relevant disease that can occur independently or secondary to other diseases such as HIV infection and AIDS. To study this latter process, we used a model in which mice are infected with the LP-BM5 murine AIDS (MAIDS) retrovirus. Cardiac function of control and infected mice was determined through the in vivo analysis of left ventricular pressure-volume loops. Furthermore, the role of myocarditis was investigated through immunohistochemistry for T-cell, B-cell, and macrophage cardiac infiltrates and Northern blot analysis for tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS). End-systolic and end-diastolic volumes were significantly increased and ventricular stiffness was significantly decreased in infected mice, consistent with DCM; however, no staining for inflammatory cellular infiltrates or TNF-α and iNOS was seen. These data support the conclusion that the LP-BM5 HIV model virus causes DCM in the absence of chronic cardiac inflammation. These findings support MAIDS retroviral infection as a new model of idiopathic DCM in which myocarditis does not occur.

Key Words

Dilated cardiomyopathy murine acquired immunodeficiency syndrome viral myocarditis 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Julie Beischel
    • 1
  • Douglas F. Larson
    • 1
  • Qianli Yu
    • 1
    • 2
  • Bo Yang
    • 1
  • Ramon Tomas Sepúlveda
    • 2
  • Tara Kelley
    • 1
  • Ronald R. Watson
    • 2
  1. 1.Sarver Heart CenterTucson
  2. 2.Arizona Prevention CenterTucson

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