Dilated cardiomyopathy in retrovirally infected mice
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Dilated cardiomyopathy (DCM) is a clinically relevant disease that can occur independently or secondary to other diseases such as HIV infection and AIDS. To study this latter process, we used a model in which mice are infected with the LP-BM5 murine AIDS (MAIDS) retrovirus. Cardiac function of control and infected mice was determined through the in vivo analysis of left ventricular pressure-volume loops. Furthermore, the role of myocarditis was investigated through immunohistochemistry for T-cell, B-cell, and macrophage cardiac infiltrates and Northern blot analysis for tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS). End-systolic and end-diastolic volumes were significantly increased and ventricular stiffness was significantly decreased in infected mice, consistent with DCM; however, no staining for inflammatory cellular infiltrates or TNF-α and iNOS was seen. These data support the conclusion that the LP-BM5 HIV model virus causes DCM in the absence of chronic cardiac inflammation. These findings support MAIDS retroviral infection as a new model of idiopathic DCM in which myocarditis does not occur.
Key WordsDilated cardiomyopathy murine acquired immunodeficiency syndrome viral myocarditis
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- 1.American Heart Association. (2002). Heart Disease and Stroke Statistics—2003 Update. American Heart Association, Dallas, TX.Google Scholar
- 12.Barbaro, G., Di Lorenzo, G., Grisorio, B., and Barbarini, G. (1998). Incidence of dilated cardiomyopathy and detection of HIV in myocardial cells of HIV-positive patients, Gruppo Italiano per lo Studio Cardiologico dei Pazienti Affetti da AIDS. N. Engl. J. Med. 339:1093–1099.PubMedCrossRefGoogle Scholar
- 18.Barbaro, G., Di Lorenzo, G., Soldini, M., Giancaspro, G., Grisorio, B., Pellicelli, A., et al. (1999). Intensity of myocardial expression of inducible nitric oxide synthase influences the clinical course of human immunodeficiency virus-associated cardiomyopathy. Gruppo Italiano per lo Studio Cardiologico dei pazienti affetti da AIDS (GISCA). Circulation 100:933–939.PubMedGoogle Scholar
- 19.Chattopadhyay, S.K., Sengupta, D.N., Fredrickson, T.N., Morse, H.C. 3rd, and Hartley, J.W. (1991). Characteristics and contributions of defective, ecotropic, and mink cell focus-inducing viruses involved in a retrovirus-induced immunodeficiency syndrome of mice. J. Virol. 65: 4232–4241.PubMedGoogle Scholar
- 21.Opie, L.H. (1998) The Heart: Physiology from Cell to Circulation. Lippincott-Raven Publishers, Philadelphia.Google Scholar
- 28.Parrillo, J.E., Aretz, H.T., Palacios, I., Fallon, J.T., and Block, P.C. (1984). The results of transvenous endomyocardial biopsy can frequently be used to diagnose myocardial diseases in patients with idiopathic heart failure: endomyocardial biopsies in 100 consecutive patients revealed a substantial incidence of myocarditis. Circulation 69:93–101.PubMedGoogle Scholar
- 32.Klingel, K., Hohenadl, C., Canu, A., Albrecht, M., Seemann, M., Mall, G., et al. (1992). Ongoing enterovirus-induced myocarditis is associated with persistent heart muscle infection: quantitative analysis of virus replication, tissue damage, and inflammation. Proc. Natl. Acad. Sci. USA 89:314–318.PubMedCrossRefGoogle Scholar