Cell Biochemistry and Biophysics

, Volume 44, Issue 3, pp 375–384 | Cite as

Molecular models of tryptophan synthase from Mycobacterium tuberculosis complexed with inhibitors

  • Marcio Vinicius Bertacine Dias
  • Fernanda Canduri
  • Nelson José Freitas da Silveira
  • Clarissa Melo Czekster
  • Luis Augusto Basso
  • Mário Sérgio Palma
  • Diógenes Santiago Santos
  • Walter Filgueira de AzevedoJr.
Original Article

Abstract

The development of new therapies against infectious diseases is vital in developing countries. Among infectious diseases, tuberculosis is considered the leading cause of death. A target for development of new drugs is the tryptophan pathway. The last enzyme of this pathway, tryptophan synthase (TRPS), is responsible for conversion of the indole 3-glycerol phosphate into indol and the condensation of this molecule with serine-producting tryptophan. The present work describes the molecular models of TRPS from Mycobacterium tuberculosis (MtTRPS) complexed with six inhibitors, the indole 3-propanol phosphate and five arylthioalkyl-phosphonated analogs of substrate of the α-subunit. The molecular models of MtTRPS present good stereochemistry, and the binding of the inhibitors is favorable. Thus, the generated models can be used in the design of more specific drugs against tuberculosis and other infectious diseases.

Index Entries

Tryptophan synthase Mycobacterium tuberculosis molecular modeling drug design structural bioinformatics 

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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Marcio Vinicius Bertacine Dias
    • 1
  • Fernanda Canduri
    • 2
  • Nelson José Freitas da Silveira
    • 1
  • Clarissa Melo Czekster
    • 3
  • Luis Augusto Basso
    • 4
  • Mário Sérgio Palma
    • 5
  • Diógenes Santiago Santos
    • 6
  • Walter Filgueira de AzevedoJr.
    • 4
  1. 1.Programa de Pós-Graduação em Biofísica Molecular-Departamento de FísicaUNESPSão José do Rio PretoBrazil
  2. 2.Departamento de Morfofisiologia-CCBS-UFMSCampo Grande-MSBrazil
  3. 3.Rede Brasileira de Pesquisas em Tuberculose, Grupo de Microbiologia Molecular e Functional, Centro de BiotecnologiaUFRGSPorto Alegre-RSBrazil
  4. 4.Faculdade de Biociências-PUCRSPorto Alegre-RSBrazil
  5. 5.Laboratory of Structural Biology and Zoochemistry, CEIS/Department of Biology, Institute of BiosciencesUNESPRio ClaroBrazil
  6. 6.Centro de Pesquisas em Biologia Molecular e Funcional/PUCRSPorto AlegreBrazil

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