Cell Biochemistry and Biophysics

, Volume 32, Issue 1–3, pp 277–281 | Cite as

Peroxisomal and mitochondrial targeting of serine: Pyruvate/alanine: Glyoxylate aminotransferase in rat liver

  • Toshiaki Oda
  • Takuji Mizuno
  • Kouichi Ito
  • Tsuneyoshi Funai
  • Arata Ichiyama
  • Satoshi Miura
Part VI Papers from Poster Session

Abstract

Serine: pyruvate/alanine:glyoxylate aminotransferase (SPT or SPT/AGT) of rat liver is a unique enzyme of dual subcellular localization, and exists in both mitochondria and peroxisomes. To characterize a peroxisomal targeting signal of rat liver SPT, a number of C-terminal mutants were constructed and their subcellular localization in transfected COS-1 cells was examined. Deletion of C-terminal NKL, and point mutation of K2 (the second Lys from the C-terminus), K4 and E15 caused accumulation of translated products in the cytoplasm. This suggests that the PTS of SPT is not identical to PTS1 (the C-terminal SKL motif) in that it is not restricted to the C-terminal tripeptide.

In vitro synthesized precursor for mitochondrial SPT was highly sensitive to the proteinase K digestion, whereas peroxisomal SPT (SPTp) was fairly resistant to the protease. In in vitro import experiment with purified peroxisomes, however, STPp recovered in the peroxisomal fraction was very sensitive to the protease. These results suggest that the mitochondrial precursor is synthesized as an unfolded form and is translocated into the mitochondrial matrix, whereas SPTp is synthesized as a folded form and its conformation changes to an unfolded form just before translocation into peroxisomes.

Index Entries

Peroxisomal targeting signal mitochondrial import subcellular translocation serine pyruvate/alanine glyoxylate aminotransferase 

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Copyright information

© Humana Press Inc 2000

Authors and Affiliations

  • Toshiaki Oda
    • 1
  • Takuji Mizuno
    • 1
  • Kouichi Ito
    • 1
  • Tsuneyoshi Funai
    • 1
  • Arata Ichiyama
    • 1
  • Satoshi Miura
    • 2
  1. 1.Department of BiochemistryHamamatsu University School of MedicineShizuokaJapan
  2. 2.Radioisotope Research CenterYokohama City University School of MedicineKanagawaJapan

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