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Biological Trace Element Research

, Volume 99, Issue 1–3, pp 173–189 | Cite as

Protective effects of ascorbic acid, Dl-α-tocopherol acetate, and sodium selenate on ethanol-induced gastric mucosal injury of rats

  • Sadakat Ozdil
  • Refiye Yanardag
  • Meral Koyuturk
  • Sehnaz Bolkent
  • Serap Arbak
Article

Abstract

In this study, the effect of ascorbic acid (vitamin C), Dl-α-tocopherol acetate (vitamin E), and sodium selenate (selenium) on ethanol-induced gastric mucosal injury in rats was investigated morphologically and biochemically. The gastric mucosal injury was produced by administration of 1 mL of absolute ethanol to each rat. Animals received vitamin C (250 mg/kg), vitamin E (250 mg/kg), and selenium (0.5 mg/kg) for 3 d 1 h prior to the administration of absolute ethanol. In gastric mucosa of rats given ethanol according to control groups, neuronal nitric oxide expression decreased. This immunoreactivity was much lower in the group given ethanol+vitamin C+vitamin E+selenium than the control group and the ethanol-induced group. Scanning electron microscopic evaluation of the ethanol-induced group, when compared to control groups, revealed degenerative changes in gastric mucosa, whereas a good arrangement in surface topography of gastric mucosa in the group given ethanol + vitamin C+vitamin E + selenium was observed. In the group administered ethanol, a reduction of the stomach glutathione (GSH) and serum total protein levels and increases in serum sialic acid, triglycerides, and stomach lipid peroxidation (LPO) levels were observed. Vitamin C+vitamin E+Se administration to alcohol-treated rats significantly increased the serum total protein, triglyceride levels, and stomach GSH levels and significantly lowered the levels of serum sialic acid and stomach LPO compared to untreated alcohol-supplemented rats. As a result of these findings, we can say that the combination of vitamin C, vitamin E, and selenium has a protective effect on ethanol-induced gastric mucosal injury of rats.

Index Entries

Ethanol gastric mucosa ascorbic acid α-tocopherol acetate sodium selenate rat 

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References

  1. 1.
    E. S. L. Liu and C. H. Cho, Relationship between ethanol-induced gastritis and gastric ulcer formation in rats, Digestion 62, 232–239 (2000).PubMedCrossRefGoogle Scholar
  2. 2.
    S. Ozdil, S. Bolkent, R. Yanardag, et al., Protective effects of ascorbic acid, Dl-α-tocopherol acetate and sodium selenate on ethanol-induced liver damage of rats, Biol. Trace Element Res., 97, 149–162 (2004).CrossRefGoogle Scholar
  3. 3.
    M. V. Suresh, S. Kumar, J. J. Lal, et al., Impact of massive ascorbic acid supplementation on alcohol induced oxidative stress in quinea pigs, Toxicol. Lett. 104, 221–229 (1999).PubMedCrossRefGoogle Scholar
  4. 4.
    G. S. Smith, D. W. Mercer, J. M. Cross, et al., Gastric injury induced by ethanol and ischemia-reperfusion in the rat, Dig. Dis. Sci. 41, 1157–1174 (1996).PubMedCrossRefGoogle Scholar
  5. 5.
    N. Sugimoto, N. Yoshida, T. Yoshikawa, et al., Effect of vitamin E on aspirin-induced gastric mucosal injury in rats, Dig. Dis. Sci. 45, 599–605 (2000).PubMedCrossRefGoogle Scholar
  6. 6.
    M. Ito, T. Segami, T. Tsukahara, et al., Effect of cimetidine and omeprazole on gastric ulcer healing of rats with limited food intake time, Eur. J. Pharmacol. 263, 245–251 (1994).PubMedCrossRefGoogle Scholar
  7. 7.
    A. R. Al-Moutary and M. Tariq, Effect of vitamin E and selenium on hypothermic restraint stress and chemically-induced ulcers, Dig. Dis. Sci. 41, 1165–1171 (1996).CrossRefGoogle Scholar
  8. 8.
    T. Yoshikawa, Y. Minamiyama, H. Ichikawa, et al., Role of lipid peroxidation and antioxidants in gastric mucosal injury induced by the hypoxanthine-xanthine oxidase system in rats, Free Radical Biol. Med. 23, 243–250 (1997).CrossRefGoogle Scholar
  9. 9.
    H. Mutoh, H. Hiraishi, S. Ota, et al., Role of oxygen radicals in ethanol-induced damage to cultured gastric mucosal cells, Am. J. Physiol. Gastrointest. Liver Physiol. 258, 603–609 (1990).Google Scholar
  10. 10.
    D. Rachmileewitz, F. Karmeli, E. Okon, et al., A novel antiulcerogenic stable radical prevents gastric mucosal lesions in rats, Gut 35, 1181–1188 (1994).Google Scholar
  11. 11.
    P. R. Kvietys, M. A. Perry, T. S. Gaginella, et al., Ethanol enhances leukocyte-endothelial cell interactions in mesenteric venules, Am. J. Physiol. Gastrointest. Liver Physiol. 259, 578–583 (1990).Google Scholar
  12. 12.
    G. N. Schrauzers. Anticarcinogenic effects of selenium, Cell. Mol. Life Sci. 57, 1864–1873 (2000).CrossRefGoogle Scholar
  13. 13.
    R. D. Baker, Jr., S. S. Baker, and R. Rao, Selenium deficiency in tissue culture: implications for oxidative metabolism, J. Pediatr. Gastroenterol. Nutr. 27, 387–392 (1998).PubMedCrossRefGoogle Scholar
  14. 14.
    K. M. Brown and J. R. Arthur, Selenium, selenoproteins and human health: a review, Public Health Nutr. 4, 593–599 (2001).PubMedGoogle Scholar
  15. 15.
    J. M. Patel and D. A. Edwards, Vitamin E, membrane order, and antioxidant behavior in lung microsomes and reconstituted lipid vesicles, Toxicol. Appl. Pharmacol. 96, 101–114 (1988).PubMedCrossRefGoogle Scholar
  16. 16.
    T. Yoshikawa, M. Yasua, S. Udea, et al., Vitamin E in gastric mucosal injury induced by ischemia-reperfusion, Am. J. Clin. Nutrit. 53, 210–214 (1991).Google Scholar
  17. 17.
    A. Carr and B. Frei, Does vitamin C act as a pro-oxidant under physiological conditions, FASEB J. 13, 1007–1024 (1999).PubMedGoogle Scholar
  18. 18.
    I. M. Drake, M. J. Davies, N. P. Mapstone, et al., Ascorbic acid may protect against human gastric cancer by scavenging mucosal oxygen radicals, Carcinogenesis 17, 559–562 (1996).PubMedCrossRefGoogle Scholar
  19. 19.
    S. Premaratne, C. Xue, J. M. McCarty, et al., Neuronal nitric oxide synthase: expression in rat parietal cells, Am. J. Physiol. 280, G308-G313 (2001).Google Scholar
  20. 20.
    S. Zöllner, R. F. Haseloff, I. A. Kirilyuk, et al., Nitroxides increase the detectable amount of nitric oxide released from endothelial cells, J. Biol. Chem. 272, 23,076–23,080 (1997).CrossRefGoogle Scholar
  21. 21.
    K. Lorentz and E. Kraas, Sialic acid in human serum and cerebrospinal fluid, J. Clin. Chem. Clin. Biochem. 24, 189–198 (1986).PubMedGoogle Scholar
  22. 22.
    A. Ledwozyw, J. Michalak, A. Stepien, et al., The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis, Clin. Chim. Acta 155, 275–284 (1986).PubMedCrossRefGoogle Scholar
  23. 23.
    E. Beutler, Glutathione in Red Blood Cell Metabolism: A Manual of Biochemical Methods, 2nd ed., Grunne and Stratton, New York, pp. 112–114 (1975).Google Scholar
  24. 24.
    O. H. Lowry, W. I. Rosebrough, A. L. Farr, et al., Protein mesurement with the Folin Phenol reagent, J. Biol. Chem. 193, 265–275 (1951).PubMedGoogle Scholar
  25. 25.
    J. L. Hintze, Copyright C, 865, East 400. North Kaysville, Utah. 84, 037 (801), 546-0445 (1986).Google Scholar
  26. 26.
    H. M. M. Arafa and M. M. Sayed-Ahmed, Protective role of carnitine esters against alcohol-induced gastric lesions in rats, Pharmacol. Res. 48, 285–290 (2003).PubMedCrossRefGoogle Scholar
  27. 27.
    G. Kanbak, M. Inal, and C. Bayçu, Ethanol-induced hepatotoxicity and protective effect of betaine, Cell Biochem. Funct. 19, 281–285 (2001).PubMedCrossRefGoogle Scholar
  28. 28.
    S. Ozdil, Protective effect of vitamin C, vitamin E and selenium on acute experimental gastric lesions induced by ethanol in the rats: a morphological study, J. Morphol. 9, 50–53 (2001).Google Scholar
  29. 29.
    R. Hernandez-Munoz, C. Montiel-Ruiz, and O. Vazquez-Martinez, Gastric mucosal cell proliferation in ethanol-induced chronic mucosal injury is related to oxidative stress and lipid peroxidation in rats, Lab. Invest. 80, 1161–1169 (2000).PubMedCrossRefGoogle Scholar
  30. 30.
    P. Navasumrit, T. H. Ward, N. J. Dodd, et al., Ethanol-induced free radicals hepatic DNA strand breaks are prevented in vivo by antioxidants: effects of acute and chronic ethanol exposure, Carcinogenesis 21, 93–99 (2000).PubMedCrossRefGoogle Scholar
  31. 31.
    K. Wingler and R. Brigelius-Floh, Gastrointestinal glutathione peroxidase, Biofactors 10, 245–249 (1999).PubMedGoogle Scholar
  32. 32.
    N. S. Parmar, M. Tariq, and M. Ageel, Gastric anti-ulcer and cytoprotective effect of selenium in rats, Toxicol. Appl. Pharmacol. 92, 122–130 (1988).PubMedCrossRefGoogle Scholar
  33. 33.
    A. G. Sivaram, M. V. Suresh, and M. Indira, Combined effect of ascorbic acid and selenium supplementation on alcohol-induced oxidative stress in guinea pigs, Comp. Biochem. Physiol. C: Toxicol. Pharmacol. 134, 397–401 (2003).CrossRefGoogle Scholar
  34. 34.
    K. Jaarin, M. Renuvathani, M. I. Nafeeza, et al., Effect of palm vitamin E on the healing of ethanol-induced gastric injury in rats, Int. J. Food Sci. Nutr. 51, S31-S41 (2000).PubMedCrossRefGoogle Scholar
  35. 35.
    K. Jaarin, M. Renuvathani, M. I. Nafeeza, et al., Comparative effect of palm vitamin E and ranitidine on the healing of ethanol-induced gastric lesions in rats, Int. J. Exp. Pathol. 80, 259–263 (1999).PubMedCrossRefGoogle Scholar
  36. 36.
    D. C. Liebler, The role of metabolism in the antioxidant function of vitamin E, Crit. Rev. Toxicol. 23, 147–169 (1993).PubMedGoogle Scholar
  37. 37.
    T. Byers and G. Perry, Dietary carotenes, vitamin C, and vitamin E as protective antioxidants in human cancers, Annu. Rev. Nutr. 12, 139–159 (1992).PubMedCrossRefGoogle Scholar
  38. 38.
    L. H. Chen, G. A. Boissonneault, and H. P. Glauert, Vitamin C, vitamin E and cancer, Anticancer Res. 8, 739–748 (1988).PubMedGoogle Scholar
  39. 39.
    A. Ceriello, D. Giugliano, A. Quatraro, et al., Vitamin E reduction of protein glycolisation in diabetes, Diabetes Care 14, 68–72 (1991).PubMedCrossRefGoogle Scholar
  40. 40.
    E. A. Lutsenko, J. M. Carcomo, and D. W. Golde, Vitamin C prevents DNA mutation induced by oxidative stress, J. Biol. Chem. 277, 16,895–16,899 (2002).CrossRefGoogle Scholar
  41. 41.
    A. Banan, J. Z. Fields, H. Decker, et al., Nitric oxide and its metabolites mediate ethanol-induced microtubule disruption and intestinal barrier dysfunction, J. Pharmacol. Exp. Ther. 294, 997–1008 (2000).PubMedGoogle Scholar
  42. 42.
    A. Berg, S. Kechagias, S. E. Sjöstrand, et al., Morphological support for paracrine inhibition of gastric acid secretion by nitric oxide in humans, Scand. J. Gastroenterol. 36, 1016–1021 (2001).PubMedCrossRefGoogle Scholar
  43. 43.
    J. F. Brown, A. C. Keates, P. J. Hanson, et al., Nitric oxide generators and cGMP stimulate mucus secretion by rat gastric mucosal cells, Am. J. Physiol. 265, G418-G422 (1993).PubMedGoogle Scholar
  44. 44.
    A. Bloodsworth, V. B. O’Donnell, and B. A. Freeman, Nitric oxide regulation of free radical- and enzyme-mediated lipid and lipoprotein oxidation, Arteriosclerosis Thromb. Vasc. Biol. 20, 1707–1715 (2000).Google Scholar
  45. 45.
    H. Kim and K. K. Hwan, Role of nitric oxide and mucus in ischemia/reperfusion-induced gastric mucosal injury in rats, Pharmacology 62, 200–207 (2001).PubMedCrossRefGoogle Scholar
  46. 46.
    M. G. Vitoria, C. G. Corchon, J. A. Rodriguez, et al., Exspression of neuronal nitric oxide synthase in several cell types of the rat gastric epithelium, J. Histochem. Cytochem. 48, 1111–1119 (2000).Google Scholar
  47. 47.
    R. Nordmann, Alcohol and antioxidant systems, Alcohol Alcohol. 29, 513–522 (1994).PubMedGoogle Scholar
  48. 48.
    J. Ren, Z. K. Roughead, L. E. Wold, et al., Increases in insulin-like growth factor-1 level and peroxidative damage after gestational ethanol exposure in rats, Pharmacol. Res. 47, 341–347 (2003).PubMedCrossRefGoogle Scholar
  49. 49.
    D. Anderson and B. J. Phillips, Comparative in vitro and in vivo effects of antioxidants, Food Chem. Toxicol. 37, 1015–1025 (1999).PubMedCrossRefGoogle Scholar
  50. 50.
    N. Sugimoto, N. Yoshida, T. Yoshikawa, et al., Effect of vitamin E on aspirin-induced gastric mucosal injury in rats, Dig. Dis. Sci. 45, 599–605 (2000).PubMedCrossRefGoogle Scholar
  51. 51.
    S. Chakraborthy, A. Nandy, M. Mukhopadhyay, et al., Ascorbate peotects guanea pigs tissue against lipid peroxidation, Free Radical Biol. Med. 16, 417–426 (1994).CrossRefGoogle Scholar
  52. 52.
    V. Balasubramaniyan, J. K. Sailaja, and N. Nalini, Role of leptin on alcohol-induced oxidative stress in Swiss mice, Pharmacol. Res. 47, 211–216 (2003).PubMedCrossRefGoogle Scholar
  53. 53.
    J. S. Park, M. A. Choi, B. S. Kim, et al., Capsaicin protects against ethanol-induced oxidative injury in the gastric mucosa of rats, Life Sci. 67, 3087–3093 (2000).PubMedCrossRefGoogle Scholar
  54. 54.
    C. Coudray, M. J. Richard, H. Faure, et al., Blood and liver lipid peroxide status after chronic ethanol administration in rats, Clin. Chim. Acta 19, 35–45 (1993).CrossRefGoogle Scholar
  55. 55.
    K. H. McDonough, Antioxidant nutritients and alcohol, Toxicology 189, 89–97 (2003).PubMedCrossRefGoogle Scholar
  56. 56.
    G. S. Muratoglu, K. Paskaloglu, S. Arbak, et al., Protective effect of famotidine, omeprazole, and melatonin against acetylsalicylic acid-induced gastric damage in rats, Dig. Dis. Sci. 46, 318–330 (2001).CrossRefGoogle Scholar
  57. 57.
    D. Bilici, H. Suleyman, Z. N. Banoglu, et al., Melatonin prevents ethanol-induced gastric mucosal damage possibly due to its antioxidant effect, Dig. Dis. Sci. 47, 856–861 (2002).PubMedCrossRefGoogle Scholar
  58. 58.
    K. J. Park, M. J. Lee, H. Kang, et al., Saeng-maek-san, a medicinal herb complex, protects liver cell damage induced by alcohol, Biol. Pharm. Bull. 25, 1451–1455 (2002).PubMedCrossRefGoogle Scholar
  59. 59.
    J. Kaur, Chronic ethanol feeding affects intestinal mucus lipid composition and glycosylation in rats, Ann. Nutr. Metab. 46, 38–44 (2002).PubMedCrossRefGoogle Scholar
  60. 60.
    M. Ponnio, H. Alho, P. Heinala, et al., Serum and saliva levels of sialic acid are elevated in alcoholics, Alcoholism: Clin. Exp. Res. 23, 1060–1064 (1999).CrossRefGoogle Scholar
  61. 61.
    J. Romppanen, K. Punnonen, P. Anttila, et al., Serum sialic acid as a marker of alcohol consumption: effect of liver disease and heavy drinking, Alcoholism: Clin. Exp. Res. 26, 1234–1238 (2002).Google Scholar

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Sadakat Ozdil
    • 1
  • Refiye Yanardag
    • 2
  • Meral Koyuturk
    • 3
  • Sehnaz Bolkent
    • 4
  • Serap Arbak
    • 5
  1. 1.Department of Internal Medicine, Istanbul Medical FacultyIstanbul UniversityCapaTurkey
  2. 2.Department of Chemistry, Faculty of EngineeringIstanbul UniversityAvcılarTurkey
  3. 3.Department of Histology and Embryology, Faculty of MedicineKadir Has UniversityGayrettepeTurkey
  4. 4.Department of Biology, Faculty of ScienceIstanbul UniversityVeznecilerTurkey
  5. 5.Department of Histology and Embryology, Marmara Medical FacultyMarmara UniversityHaydarpasa, IstanbulTurkey

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