Selenium supplementation on plasma glutathione peroxidase activity in patients with end-stage chronic renal failure
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Patients with chronic renal failure (CRF) usually have a lower than healthy level of selenium (Se) in whole blood and plasma. Plasma glutathione peroxidase (GSH-Px) is synthesized mostly in the kidney. In CRF patients, activity of this enzyme is significantly reduced and its reduction increases with the progress of the disease. The aim of the study was to evaluate the effect of Se supplementation to CRF patients at various stages of the disease on Se concentration in blood components and on plasma GSH-Px activity.
The study group comprised 53 CRF patients at various stages of the disease supplemented with Se (200 µg/d for 3 mo as Se-enriched yeast, containing about 70% l-selenomethionine [SeMet]). The control group consisted of 20 healthy subjects. The Se concentration in blood components was measured spectrofluorometrically with 2,3-diaminonaphthalene as a complexing reagent. GSH-Px activity in red cell hemolysates and plasma was assayed by the coupled method with tert-butyl hydroperoxide as a substrate.
The Se concentration in whole blood and plasma of CRF patients is significantly lower as compared with healthy subjects, but similar at all stages of the disease. In the patients’ plasma, total protein and albumin levels are also significantly lower than in healthy subjects. Plasma GSH-Px activity in patients is extremely low, and contrary to Se concentration, it decreases linearly with the increasing stage of the illness. Se-supplied patients show an increased Se concentration in all blood components and at all disease stages, whereas plasma GSH-Px activity is enhanced only at the incipient stage of the disease. Se supply has no effect on plasma GSH-Px activity in uremic patients at the end stage of the disease. Total plasma protein and albumin levels did not change after Se supplementation. Our data seem to show that in patients with CRF lower total protein and albumin levels in plasma may be the chief cause of the low blood and plasma Se concentrations. GSH-Px activity decreases along with the kidney impairment. At the end stage of the disease, Se supplementation in the form of Se-enriched yeast has no effect on the increase in plasma GSH-Px activity.
Index EntriesBlood chronic renal failure glutathione peroxidase plasma selenium
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- 1.M. P. Rayman, Dietary selenium: time to act, Br. Med. J. 314, 387–388 (1997).Google Scholar
- 16.B. A. Zachara, A. Trafikowska, J. Manitius, et al., Third International Meeting “Advances in Trace Elements, Minerals and Vitamins in Humans: Functional and Clinical Aspects,” Monastir, Tunisia 2002, Abstracts p. 49.Google Scholar
- 22.B. A. Zachara, D. Koterska, A. Trafikowska, et al., The effect of selenium supplementation on plasma glutathione peroxidase activity in patients with different stages of chronic renal failure, in Macro and Trace Elements; 21 Workshop, M. Anke, R. Muller, U. Schafer, and M Stoeppler, eds., 2002, pp. 945–951.Google Scholar
- 23.B. A. Zachara, M. Sklodowska, W. Wasowicz, et al., Selenium status, glutathione peroxidase activity and lipid peroxides concentration in blood of Polish population. III. Effect of age and sex in healthy adults, in Elements in Health and Disease, M. Said, M. A. Rahman, and L. A. D’Silva, eds., Hamdard University Press, Karachi, Pakistan, pp. 501–512 (1989).Google Scholar
- 29.B. A. Zachara and W. Wasowicz, Selenium concentration in blood components of Polish sub-population and in some other countries, in Arsenium and Selenium in the Environment—Ecological and Methodological Problems, A. Kabata-Pendias, and B. Szteke, eds., Polish Academy of Sciences, Warsaw, pp. 157–166 (1994) (in Polish).Google Scholar
- 30.G.-Q. Yang, L.-Z. Zhu, S.-J. Liu, et al., Human selenium requirement in China, in Selenium in Biology and Medicine, Part B, G. F. Combs, Jr., J. E. Spallholz, O. A. Levander, and J. E. Oldfield, eds., Van Nostrand Reinhold, New York, pp. 589–607 (1987).Google Scholar
- 31.A. Madaric, J. Kadrabova, and E. Ginter, Selenium concentration in plasma and erythrocytes in healthy Slovak population, J. Trace Elements Electrolytes Health Dis. 8, 43–47 (1994).Google Scholar
- 33.B. Tiran, A. Tiran, W. Petek, et al., Selenium status of healthy children and adults in Styria (Austria). Trace Elements Med. 9, 75–79 (1992).Google Scholar
- 34.J. Kalouskova, J. Dedina, L. Pavlik, et al., Selenium concentration in blood of the Czech Population, Cas. Lek. Ces. 127, 277–281 (1987).Google Scholar
- 35.G. Alfthan, G. Bogye, A. Aro, et al., The human selenium status in Hungary, J. Trace Elements Electrolytes Health Dis. 6, 233–238 (1992).Google Scholar
- 38.J. M. Marchante-Gayon, J. E. Sanchez-Uria, and A. Sanz-Medel, Serum and tissue selenium contents related to renal disease and colon cancer as determined by electrothermal atomic absorption spectrometry, J. Trace Elements Med. Biol. 10, 229–236 (1996).Google Scholar
- 40.K. A. Temple, A. M. Smith, and D. B. Cockram, Selenate-supplemented nutritional formula increases plasma selenium in hemodialysis patients, J. Renal Nutr. 10, 16–23 (2000).Google Scholar
- 43.R. Read, T. Bellow, J.-G. Yang, et al., Selenium and amino acid composition of selenoprotein P, the major selenoprotein in rat serum, J. Biol. Chem. 265, 17,899–17,905 (1990).Google Scholar
- 44.M. J. Richard, J. Arnaud, K. Sirajeddine, et al., Zinc, selenium and lipid peroxides levels in hemodialyzed patients, in Trace Elements in Man and Animals 7 (TEMA-7), B. Momcilovic (ed.), University of Zagreb, Zagreb, pp. 12.7–12.9 (1991).Google Scholar
- 45.G. Bellisola, G. C. Guidi, G. Cinque, et al., Selenium status and plasma glutathione peroxidase in patients with IgA nephropathy, J. Trace Elements Med. Biol. 10, 189–196 (1996).Google Scholar
- 49.M. D. Saint-Georges, D. J. Bonnefont, B. A. Bourlery, et al., Correction of selenium deficiency in hemodialyzed patients, Kidney Int. 36 (Suppl. 27), S-274–S-277 (1989).Google Scholar
- 50.G. Bellisola, G. Perona, S. Galassini, et al., Plasma selenium and glutathione peroxidase activities in individuals living in the Veneto region of Italy, J. Trace Elements Electrolytes Health Dis. 7, 242–244 (1993).Google Scholar