Biological Trace Element Research

, Volume 96, Issue 1–3, pp 255–262 | Cite as

Effects of zinc and melatonin deficiency on testicular tissue of rats

  • Ahmet Oztürk
  • Abdulkerim Kasim Baltaci
  • Cem Seref Bediz
  • Rasim Mogulkoc
  • Salim Güngör


The present study was designed to investigate the effects of zinc and/or melatonin deficiency on rat testes. A total of 24 adult male Sprague-Dawley rats were used in this study. The rats were divided into four groups of six rats each, as follows: (I) controls, (II) zinc deficient, (III) pinealectomized, zinc normal, and (IV) pinealectomized, zinc deficient. The plasma zinc levels in the control group were higher than in all the other groups (p<0.01), and those of the zinc-deficient groups II and IV were significantly lower than for group III (p<0.01). The melatonin levels in the controls were also significantly higher than for all other groups (p<0.01) There was no significant difference in sperm production between the controls and the group of animals that had no epiphysis. A significant suppression was observed in the spermatogenetic activity of the zinc-deficient groups (p<0.01). The suppression was higher in group II than in group IV. These results indicate that testicular damage caused by zinc deficiency may be reduced by melatonin deficiency.

Index Entries

Melatonin pinealectomy rats testes zinc deficiency 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. W. Hadden, Thymic endocrinology, Ann. NY Acad. Sci. 1, 352–358 (1998).CrossRefGoogle Scholar
  2. 2.
    L. Li, J. T. Y. Wong, S. F. Pang, et al., Melatonin-induced stimulation of rat corpus epididymal epithelial cell proliferation, Life Sci. 65, 1067–1076 (1999).PubMedCrossRefGoogle Scholar
  3. 3.
    G. Chazot, B. Claustrat, J. Brun, et al., Rapid antidepressant activity of destyro gamma endrophin: correlation with urinary melatonin, Biol. Psychiatry 20, 1026–1030 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    G. J. M. Maestroni, The immunoneuroendocrine role of melatonin, J. Pineal Res. 14, 1–10 (1993).PubMedCrossRefGoogle Scholar
  5. 5.
    A. S. Prasad, Zinc and immunity, Mol. Cell. Biochem. 188, 63–69 (1998).PubMedCrossRefGoogle Scholar
  6. 6.
    Y. Nishi, Zinc and growth, J. Am. Coll. Nutr. 15, 59–63 (1996).Google Scholar
  7. 7.
    Y. Liu, R. B. Franklin, and L. C. Costello, Prolactin and testosterone regulation of mitochondrial zinc in prostate epithelial cells, Prostate 30, 26–32 (1997).PubMedCrossRefGoogle Scholar
  8. 8.
    S. A. Hamdi, O. I. Nassif, and M. S. Ardawi, Effect of marginal or severe dietary zinc deficiency on testicular development and functions of the rat, Arch. Androl. 38, 243–253 (1997).PubMedGoogle Scholar
  9. 9.
    R. S. Bedwal and A. Bahuguna, Zinc, copper and selenium in reproduction, Experientia 50, 626–640 (1994).PubMedCrossRefGoogle Scholar
  10. 10.
    T. Suzuki, K. Suzuki, K. Nakajima, et al., Metallothionein in human seminal plasma, Int. J. Urol. 1, 345–348 (1994).PubMedCrossRefGoogle Scholar
  11. 11.
    C. S. Bediz, A. K. Baltacı, A. M. Tiftik, et al., Effects of zinc deficiency on some hormones in rats, Selcuk J. Med. 15, 59–63 (1999).Google Scholar
  12. 12.
    J. Kuszak and M. A. Rodin, New technique of pinealectomy for adult rats, Proc. Exp. 33, 283–284 (1977).CrossRefGoogle Scholar
  13. 13.
    S. G. Johnsen, Testicular biopsy score count: a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males, Hormones 1, 2–25 (1970).PubMedCrossRefGoogle Scholar
  14. 14.
    A. E. Pekary, C. H. Lukaski, I. Mena, et al., Testosterone increases TRH biosynthesis in epididymis but not heart of zinc-deficient rats, Peptides 14, 315–324 (1993).PubMedCrossRefGoogle Scholar
  15. 15.
    A. S. Prasad, C. S. Mantzoros, F. W. J. Beck, et al., Zinc status and serum testosterone levels of healthy adults, Nutrition 12, 344–348 (1996).PubMedCrossRefGoogle Scholar
  16. 16.
    E. Mocchegiani, D. Bulian, L. Santarelli, et al., The zinc pool is involved in the immune-reconstituting effect of melatonin in pinealectomized mice, J. Pharmacol. Exp. Ther. 277, 1200–1208 (1996).PubMedGoogle Scholar
  17. 17.
    P. G. Reeves and L. Stallard, Zinc deficiency reduces the activity of angiotensin-converting enzyme in testicular germ cells and sperm of adult rats, J. Trace Elements Exp. Med. 7, 125–134 (1995).Google Scholar
  18. 18.
    A. A. Hafiez, Z. H. M. El-Kirdassy, N. M. El-Malkh, et al., Role of zinc in regulating the testicular function. Part 3. Histopathological changes induced by dietary zinc deficiency in testes of male albino rats, Die Nahrung 34, 65–73 (1990).PubMedCrossRefGoogle Scholar
  19. 19.
    M. K. Vaughan, S. Oaknin, B. Cozzi, et al., Influence of melatonin on the testicular regresion induced by subcutaneous testosterone pellets in male rats kept in long or short photoperiod, J. Reprod. Fertil. 82, 277–284 (1988).PubMedCrossRefGoogle Scholar
  20. 20.
    J. F. Jarrige and D. Boucher, Influence de la glande pineale maternelle sur la fonction testiculaire du rat, Ann. Endocrinol. 53, 67–70 (1992).Google Scholar
  21. 21.
    B. Gunduz and M. H. Stetson, Effects of photoperiod, pinealectomy and melatonin implants on testicular development in juvenile Siberian hamster (Phodopus sungorus), Biol. Reprod. 51, 1181–1187 (1994).PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Ahmet Oztürk
    • 1
  • Abdulkerim Kasim Baltaci
    • 2
  • Cem Seref Bediz
    • 4
  • Rasim Mogulkoc
    • 2
  • Salim Güngör
    • 3
  1. 1.Department of Urology, Medical SchoolSelcuk UniversityKonyaTurkey
  2. 2.Department of Physiology, Medical SchoolSelcuk UniversityKonyaTurkey
  3. 3.Department of Pathology, Medical SchoolSelcuk UniversityKonyaTurkey
  4. 4.Department of Physiology, Medical SchoolDokuz Eylul UniversityIzmirTurkey

Personalised recommendations