Biological Trace Element Research

, Volume 83, Issue 1, pp 49–55 | Cite as

Chemotactic responses of osteoblastic MC3T3-E1 cells toward zinc chloride

  • Sanae Kanno
  • C. D. Anuradha
  • Seishiro Hirano


Although zinc (Zn) is known to participate in bone formation, its exact role in the remodeling of this tissue has not been fully clarified. The present study was designed to investigate whether Zn has a role at the resorptive sites in vitro. We investigated the migration of osteoblastic MC3T3-E1 cells in response to Zn using a Boyden chamber assay. Exposure of MC3T3-E1 cells to Zn stimulated the migration of MC3T3-E1 cells. Checkerboard analysis revealed that the migration of MC3T3-E1 cells toward Zn was a directional (chemotaxis) rather than a random (chemokinesis) motion.

Pretreatment of MC3T3-E1 cells with pertussis toxin completely blocked the chemotactic response of cells to Zn, indicating that it is mediated by G-protein-coupled receptors. Because the bone is one of the major Zn storage sites, we suggest that Zn released from bone-resorptive sites plays an important role in the recruitment of osteoblasts and bone renewal.

Index Entries

Zinc MC3T3-E1 chemotaxis bone renewal G-protein calcium platelet-derived growth factor 


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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Sanae Kanno
    • 1
  • C. D. Anuradha
    • 1
  • Seishiro Hirano
    • 1
  1. 1.Research Center for Environmental RiskNational Institute for Environmental StudiesIbarakiJapan

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