Biological Trace Element Research

, Volume 76, Issue 3, pp 245–256 | Cite as

Effects of acute and chronic coingestion of AlCl3 with citrate or polyphenolic acids on tissue retention and distribution of aluminum in rats

  • Zeyuan Deng
  • Charles Coudray
  • Laurent Gouzoux
  • Andrzej Mazur
  • Yves Rayssiguier
  • Denise Pépin


Aluminum (Al) is toxic to certain biological systems and has been implicated as a neurotoxic agent in the pathogenesis of Alzheimer’s disease. Intestinal absorption of Al is very low (0.1%), but many organic dietary components are potential chelators of Al and may enhance its absorption and tissue distribution. We examined the effects of acute and chronic coingestion of AlCl3 with different polyphenolic acids on Al retention and compared to citrate in rats. In experiment 1, animals fasted for 14 h were dosed orally with demineralized water, Al chloride, Al chloride plus sodium citrate, or Al chloride plus a polyphenol acid. Blood samples were taken before and 2 h after the gavage and animals were killed 6 h later. In experiment 2, the rats were adapted on a purified diet for 1 wk and received the following for 4 wk in their experimental diets: AlCl3, except group 1, plus citrate or a polyphenol acid, except groups 1 and 2. Animals were killed and blood and tissues were sampled. In experiment 1, citrate highly enhanced Al absorption and its tissue retention. Gallic and chlorogenic acids significantly increased tibia and kidney Al levels compared to the Al group. In experiment 2, Al levels in the urine were significantly increased in all the Al groups compared to the control group. Significantly higher Al levels in the tibia, kidney, and brain were observed in the citrate group and a significant increase in brain Al level was also noted in the chlorogenic acid group compared to AlCl3 group. This may suggest a possible relation structure-activity of polyphenol acids. However, further studies are necessary to better understand the influence of polyphenol acids on Al metabolism, in particular that of chlorogenic acid.

Index Entries

Aluminum absorption toxicity polyphenol citrate rat 


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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Zeyuan Deng
    • 1
  • Charles Coudray
    • 1
  • Laurent Gouzoux
    • 2
  • Andrzej Mazur
    • 1
  • Yves Rayssiguier
    • 1
  • Denise Pépin
    • 2
  1. 1.Centre de Recherche en Nutrition Humaine d’Auvergne, Unité Maladies Métaboliques et MicronutrimentsINRA Center de Clermont-Ferrand/TheixSaint Genès ChampanelleFrance
  2. 2.Laboratoire de Contrôle des EauxInstitut Louise Blanquet, Faculté Médecine et PharmacieClermont-FerrandFrance

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