Determination of selenium in serum by FI-HG-AAS and calculation of dietary intake
A method was developed for the determination of selenium concentration in serum by flow injection-hydride generation-atomic absorption spectrometry (FI-HG-AAS) following microwave digestion of serum samples and reduction of selenate to selenite. The detection limit of the method was 0.3 µg Se/L and the characteristic concentration, corresponding to the 0.0044 absorbance signal, was 0.12 µg Se/L. The results from the analysis of two Seronorm standard reference materials showed good agreement with the certified values. The method was then used to analyze selenium in sera of Austrian and Slovenian people for the calculation of dietary intakes. The selenium concentrations in sera of mothers at delivery, their neonates, and the male and female adults were 71 ± 14, 42 ± 6, 75 ± 21, and 65 ± 16 µg/L for the Austrians and 62 ± 15, 34 ± 7, 70 ± 12, and 66 ± 15 µg/L for the Slovenians. The dietary intakes of selenium of the mothers and the male and the female adults were calculated as 52, 37, and 46 µg/d for the Austrians and 45, 38, and 32 µg/d for the Slovenians.
Index EntriesSelenium Austria Slovenia serum dietary intake FI-HG-AAS mircrowave digestion
Unable to display preview. Download preview PDF.
- 1.X. Chen, G. Yang, J. Chen, X. Chen, Z. Wen, and K. Ge, Studies on the relationship of selenium and Keshan disease, Biol. Trace Elem. Res. 2, 91–107 (1980).Google Scholar
- 2.World Health Organization, Environmental Health Criteria 58, Selenium, WHO, Geneva (1987).Google Scholar
- 3.Food and Nutrition Board, National Research Council, Recommended Dietary Allowances, National Academy of Sciences, Washington, DC (1989).Google Scholar
- 4.M. Haldimann, T. Y. Venner, and B. Zimmerli, Determination of selenium in the serum of healthy Swiss adults and correlation to dietary intake, J. Trace Elements Med. Biol. 10, 31–45 (1996).Google Scholar
- 5.J. Dedina and D. L. Tsalav, Hydride Generation Atomic Absorption Spectrometry, Wiley, Chichester (1995).Google Scholar
- 7.D. Mayer, S. Haubenwallner, W. Kosmus, and W. Beyer, Modified electrical heating-system for hydride generation atomic-absorption spectrometry and elaboration of a digestion method for the determination of arsenic and selenium in biological-materials, Anal. Chim. Acta 268, 315–321 (1992).CrossRefGoogle Scholar
- 11.V. E. Negretti de Braetter, P. Braetter, and A. Tomiak, An automated microtechnique for selenium determination in human-body fluids by flow-injection hydride generation atomic-absorption spectrometry (FI-HG-AAS), J. Trace Element Elect. Health Dis. 4, 41–48 (1990).Google Scholar
- 12.B. Tiran, A. Tiran, E. Rossipal, and O. Lorenz, Simple decomposition procedure for determination of selenium in whole-blood, serum and urine by hydride generation atomic-absorption spectroscopy, J. Trace Element Elect. Health Dis. 7, 211–216 (1993).Google Scholar
- 13.J. Versieck and R. Cornelis, Trace Elements in Human Plasma or Serum, CRC, Boca Raton, FL (1989).Google Scholar
- 14.P. Varo, G. Alfthan, J. K. Huttunen, and A. Aro, Nationwide selenium supplementation in Finland—Effects on diet, blood and tissue levels, and health, in Selenium in Biology and Human Health, R. F. Burk, ed., Springer-Verlag, New York, pp. 209–210 (1994).Google Scholar
- 16.L. Magos and G. G. Berg, in Biological Monitoring of Toxic Metals, T. W. Clarkson, L. Friberg, G. F. Nordberg, and P. R. Sager, eds., Plenum, New York, pp. 383–405 (1988).Google Scholar
- 17.W. Pfannhauser, Essentielle Spurenelemente in der Nahrung, Springer-Verlag, Berlin (1988).Google Scholar