Biological Trace Element Research

, Volume 113, Issue 2, pp 165–175 | Cite as

Effects of different proteins on the metabolism of Zn, Cu, Fe, and Mn in rats

  • Guitang Chen
  • Lin Zhao
  • Shanfen Bao
  • Tao Cong


Many factors are known to influence trace element metabolism and one of them is dietary protein. The present study examines the effects of casein, soybean protein, and peanut protein on the metabolism of the Zn, Cu, Fe, and Mn in growing rats. The results showed that Zn, Fe, and Mn excretions in the feces of peanut protein-fed rats (PPERs) were similar to that of casein-fed rats (CPFRs) (p>0.05), whereas all of the Zn, Cu, Fe, and Mn excretions in the urine of PPFRs were significantly higher than that of CPFRs (p<0.05), but its apparent absorption rate (AAR) of Cu, Fe and its apparent retention rate (ARR) of Cu were all higher than that of CPFRs (p<0.05). Hepatic Zn content of soybean protein-fed rats (SPFRs) was higher than that of CPFRs and PPFRs (p<0.05 respectively) and serum, renal, and femoral Cu contents of SPFRs were significantly lower; however, hepatic Cu, and renal Mn contents were significantly higher than that of CPFRs (p<0.05, respectively); The hepatic Fe content of SPFRs was significantly higher than that of CPFRs and PPFRs (p<0.01, respectively). To sum up, compared to casein, soybean protein might be a good dietary source to make up for Zn and Fe deficiency, and also peanut protein to make up for Cu and Fe deficiency.

Index Entries

Peanut protein trace elements rat casein soybean protein 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J. L. Greger and S. M. Snedeker, Effect of dietary protein and phosphorus levels on the utilization of zinc, copper and manganese by adult males, J. Nutr. 110, 2243–2253 (1980).PubMedGoogle Scholar
  2. 2.
    S. B. Zhou, G. J. Wang, S. Y. Yu, and C. Y. Bao, The effect of different protein intake levels on the utilization of protein, zinc, iron and copper in rats, Acta Nutr. Sin. 17, 135–140 (1995).Google Scholar
  3. 3.
    H. Y. Neggers and S. Jarnail, Effect of dietary protein, zinc, concentration in mice, Biol. Trace Element Res., 98, 171–179 (2004).CrossRefGoogle Scholar
  4. 4.
    C. Wang, Y. Q. Guan, X. M. Hu, and X. Z. Wen, Study on relation of different proteins and zinc metabolism in rats, Chin. J. Food Hyg. 15, 305–307 (2003).Google Scholar
  5. 5.
    G. R. Philip, H. N. Forrest, and C. F. George, AIN-93G purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet, J. Nutr. 123, 1939–1951 (1993).Google Scholar
  6. 6.
    B. Sandstrom, A. Almgren, and B. Kivisto, Effect of protein level and protein source on zinc absorption in human, Nutrition 119, 48 (1989).Google Scholar
  7. 7.
    R. A. Wapnir, Copper absorption and bioavailability, Am. J. Clin. Nutr. 67(Suppl.), 1054–1060 (1998).Google Scholar
  8. 8.
    W. Ingeborg, F. B. Xandra, and L. Egidius, Liver copper content of rats hypo- or hyper-responsive to dietary cholesterol J. Trace Elements Med. Biol. 17, 177–182 (2002).Google Scholar
  9. 9.
    M. C. Linder and M. Roboz, Turnover and excretion of copper in rats as measured with 67Cu, Am. J. Physiol. 251, 551–555 (1986).Google Scholar
  10. 10.
    L. G. Strause, J. Hegenauer, and P. Saltman, Effects of long-term dietary manganese and copper deficiency on rat skeleton, J. Nutr. 116, 135–141 (1986).PubMedGoogle Scholar
  11. 11.
    R. Uauy, M. Olivares, and M. Conzalez, Essentiality of copper in humans, Am. J. Clin. Nutr. 67(Suppl.), 952–959 (1998).Google Scholar
  12. 12.
    C. D. Yee, S. K. Karen, and M. Walker, The relationship of nutritional copper to the development of postmenopausal osteoporosis in rats, Biol. Trace Element Res. 48, 1–11 (1995).CrossRefGoogle Scholar
  13. 13.
    F. E. Viteri, The consequence of iron deficiency and anemia in pregnancy on maternal health the foetus and the infant, Sci. News 11, 14–17 (1994).Google Scholar
  14. 14.
    FAO, Assessment of the prevalence and causes of anemia, in Agriculture, Food and Nutrition for Africa, FAO, Rome, pp. 296–304 (1997).Google Scholar
  15. 15.
    F. Wedler, Biochemical and nutritional role of manganese: an overview, in Manganese in Health and Disease, D. Klimis-Tavantzis, ed., CRC, Boca Raton, FL, pp. 1–38 (1994).Google Scholar
  16. 16.
    J. W. Finley, P. E. Johnson, and L. K. Johnson, Sex affects manganese absorption and retention by humans from a diet adequate in manganese, Am. J. Clin. Nutr. 60, 949–955 (1994).PubMedGoogle Scholar
  17. 17.
    C. Davis, T. Wolf, and J. Greger, Varying levels of manganese and iron affect absorption and gut endogenous losses of manganese by rats, J. Nutr. 122, 1300–1308 (1992).PubMedGoogle Scholar
  18. 18.
    D. Y. Lee and P. E. Johnson, Factors affecting absorption and excretion of 54Mn in rats, J. Nutr. 118, 1509–1516 (1988).PubMedGoogle Scholar
  19. 19.
    L. Davidsson, A. Almgren, M. A. Juillerat, and R. F. Hurrell, Manganese absorption in humans: the effect of phytic acid and ascorbic acid in soybean formula, Am. J. Clin. Nutr. 62, 984–987 (1995).PubMedGoogle Scholar
  20. 20.
    R. N. Wapnir, Protein Nutrition and Mineral Absorption, CRC, Boca Raton, FL (1990).Google Scholar
  21. 21.
    K. J. Wedekind, E. C. Titgemeyer, A. R. Twardock, and D. H. Baker, Phosphorus, but not calcium, affects manganese absorption and turnover in chicks, J. Nutr. 121, 1776–1786 (1991).PubMedGoogle Scholar
  22. 22.
    L. Davidsson, A. Cederblad, B. Lönnerdal, and B. Sandstrom, The effect of individual dietary components on manganese absorption in humans, Am. J. Clin. Nutr. 54, 1065–1070 (1991).PubMedGoogle Scholar
  23. 23.
    F. R. Hurrell, Influence of vegetable protein sources on trace element and mineral bioavailability, J. Nutr. 133, 2973–2977 (2003).Google Scholar
  24. 24.
    J. R. Turnlund, J. C. King, B. Gong, and M. C. Michel, A stable isotope study of copper absorption in young man: effects of phytate and alpha-cellulose, Am. J. Clin. Nutr. 42, 18–23 (1985).PubMedGoogle Scholar
  25. 25.
    W. F. John, Manganese absorption and retention by young women is associated with serum ferritin concentration, Am. J. Clin. Nutr. 70, 37–43 (1990).Google Scholar

Copyright information

© Humana Press Inc 2006

Authors and Affiliations

  • Guitang Chen
    • 1
    • 2
  • Lin Zhao
    • 2
  • Shanfen Bao
    • 2
  • Tao Cong
    • 2
  1. 1.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingPeople's Republic of China
  2. 2.Department of NutritionChinese PLA General HospitalBeijingPeople's Republic of China

Personalised recommendations