Biological Trace Element Research

, Volume 112, Issue 3, pp 241–246 | Cite as

Increased micronucleus frequency after oral administration of cadmium in dogs

  • Hamiyet Dönmez-Altuntas
  • Zuhal Hamurcu
  • Narin Liman
  • Halil Demirtas
  • Nalan Imamoglu
Original Articles

Abstract

Cadmium (Cd) is a toxic heavy metal that has been classified as a human carcinogen by the International Agency for Research on Cancer. The genotoxic effects of cadmium oxide (CdO) were investigated in cultured dog lymphocytes after a short-term oral CdO administration by the micronucleus (MN) test. The dogs were given 10 mg CdO/kg body weight per day for 3 and 28 d, respectively group I (n=7) and group II (n=6). Blood samples were collected at the beginning of feeding and at 4 and 29 d after Cd administration and cultured for 72 h. Whereas no significant increase in the MN frequency in group I was observed (p=0.398), a significant MN induction with CdO was found in group II (p=0.028) when compared with initial MN frequencies of dogs in both groups. Our results suggest that CdO might be directly and/or indirectly genotoxic after a monthly oral administration of CdO in dogs.

Index Entries

Cadmium cadmium oxide dogs feed micronucleus 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    IARC (International Agency for Research on Cancer), Beryllium, cadmium, mercury, and exposures in the glass manufacturing industry, in IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Volume, 58. IARC Scientific Publications Lyon, pp 119–238 (1993).Google Scholar
  2. 2.
    C. G. Elinder, T. Kjellstorm, C. Hogstedt, K. Andersson, and G. Spong, Cancer mortality of cadmium workers, Br. J. Ind. Med. 42, 651–655 (1985).PubMedGoogle Scholar
  3. 3.
    M. Waisberg, P. Joseph, B. Hale, and D. Beyersmann, Molecular and cellular mechanisms of cadmium carcinogenesis, Toxicology 192, 95–117 (2003).PubMedCrossRefGoogle Scholar
  4. 4.
    M. P. Waalkes, Cadmium carcinogenesis in review, J. Inorg. Biochem. 79, 241–244 (2000).PubMedCrossRefGoogle Scholar
  5. 5.
    M. P. Waalkes, Cadmium carcinogenesis, Mutat. Res. 533, 107–120 (2003).PubMedGoogle Scholar
  6. 6.
    V. Verougstraete, D. Lison, and P. Hotz, A systematic review of cytogenetic studies conducted in human populations exposed to cadmium compounds, Mutat. Res. 511, 15–43 (2002).PubMedCrossRefGoogle Scholar
  7. 7.
    J. Y. Fu, X. S. Huang, and X. Q. Zhu, Study on peripheral blood lymphocytes chromosome abnormality of people exposed to cadmium in environment, Biomed. Environ. Sci. 12, 15–19 (1999).PubMedGoogle Scholar
  8. 8.
    A. Forni, Comparison of chromosome aberrations and micronuclei in testing genotoxicity in humans, Toxicol. Lett. 72, 185–190 (1994).PubMedCrossRefGoogle Scholar
  9. 9.
    K. Nogawa, I. Tsuritani, Y. Yamada, et al., Sister chromatid exchanges in the lymphocytes of people exposed to environmental cadmium, Toxicol. Lett. 32, 283–288 (1986).PubMedCrossRefGoogle Scholar
  10. 10.
    H. C. Wulf, N. Kromann, N. Kousgaard, J. C. Hansen, E. Niebuhr, and K. Alboge, Sister chromatid exchange (SCE) in Greenlandic Eskimos: dose-response relationship between SCE and seal diet, smoking and blood cadmium and mercury concentrations, Sci. Total. Environ. 48, 81–94 (1986).PubMedCrossRefGoogle Scholar
  11. 11.
    M. Fenech and A. A. Morley, Measurement of micronuclei in lymphocytes, Mutat. Res. 147, 29–36 (1985).PubMedGoogle Scholar
  12. 12.
    A. N. Balasem and A. Ali, Establishment of dose-response relationships between doses of CS-317 γ-rays and frequencies of micronuclei in human peripheral blood, Mutat. Res. 259, 133–138 (1991).PubMedCrossRefGoogle Scholar
  13. 13.
    M. Fenech, The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human populations, Mutat. Res. 285, 35–44 (1993).PubMedGoogle Scholar
  14. 14.
    M. Fenech, The in vitro micronucleus technique, Mutat. Res. 455, 81–95 (2000).PubMedGoogle Scholar
  15. 15.
    A. Hartwig and T. Schwerdtle, Interactions by carcinogenic metal compounds with DNA repair processes: toxicological implications, Toxicol. Lett. 127, 47–54 (2002).PubMedCrossRefGoogle Scholar
  16. 16.
    R. R. Misra, G. T. Smith, and M. P. Waalkes, Evaluation of the direct genotoxic potential of cadmium in four different rodent cell lines, Toxicology, 126, 103–114 (1998).PubMedCrossRefGoogle Scholar
  17. 17.
    L. C. Backer, C. B. Grindem, W. T. Corbett, L. Cullins, and J. L. Hunter, Pet dogs as sentinels for environmental contamination, Sci. Total. Environ. 274, 161–169 (2001).PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Hamiyet Dönmez-Altuntas
    • 1
  • Zuhal Hamurcu
    • 1
  • Narin Liman
    • 2
  • Halil Demirtas
    • 1
  • Nalan Imamoglu
    • 1
  1. 1.Department of Medical Biology, Medical FacultyErciyes University KayseriTurkey
  2. 2.Department of Histology and Embryology, Veterinary FacultyErciyes University KayseriTurkey

Personalised recommendations