Biological Trace Element Research

, Volume 108, Issue 1–3, pp 225–243 | Cite as

Metal tissue kinetics in regenerating liver, thymus, spleen, and submandibular gland after partial hepatectomy in mice

  • Čedomila Milin
  • Marin Tota
  • Robert Domitrović
  • Jasminka Giacometti
  • Radojka Pantovic
  • Mira Ćuk
  • Ines Mrakovčić-Šutić
  • Hrvoje Jakovac
  • Biserka Radošević-Stašić
Original Articles


Liver regeneration after partial hepatectomy (pHx) is a well-defined process, which involves the concerted action of extra- and intracellular factors resulting in induction of cell replication and its inhibition at the time when the entire liver mass is restored. Concomitantly, the breakdown of previously maintained tolerance and the exposure of self-antigens lead to the activation of preimmune and immune repertoires, which participate in surveillance against aberrant cells and the re-establishment of previous morphostasis. Because, in these events, important biological function might have tissue minerals that are affecting the structural integrity and enzyme activities, transduction signals, transcription and replication factors during cell proliferation and apoptosis, as well as the development and maintenance of immune functions and cytokine production, in this study we analyzed tissue dynamics of zinc, ioron magnesium, and calcium in the liver, thymus, spleen, and submandibular gland in intact and pHx mice on the 1st, 2nd, 7th, and 15th d after one-third pHx, using microwave digestion and inductivity coupled plasma spectrometry. The data showed that pHx induces significant and interconnected changes in all of the estimated metals not only in the regenerating liver but also in the lymphatic tissues and submandibular gland, indicating their importance for the control of growth processes.

Index Entries

Zinc iron calcium magnesuim ICP spectroscopy atomic absorption partial hepatectomy liver regeneration thymus spleen submandibular gland 


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Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Čedomila Milin
    • 1
  • Marin Tota
    • 1
  • Robert Domitrović
    • 1
  • Jasminka Giacometti
    • 1
  • Radojka Pantovic
    • 1
  • Mira Ćuk
    • 2
  • Ines Mrakovčić-Šutić
    • 2
  • Hrvoje Jakovac
    • 2
  • Biserka Radošević-Stašić
    • 2
  1. 1.Department of Chemistry and BiochemistryMedical Faculty of RijekaRijekaCroatia
  2. 2.Department of Physiology and ImmunologyMedical Faculty of RijekaRijekaCroatia

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