Biological Trace Element Research

, Volume 107, Issue 2, pp 135–140 | Cite as

Twenty-eight element concentrations in mane hair samples of adult riding horses determined by particle-induced X-ray emission

  • Kimi Asano
  • Kazuyuki Suzuki
  • Momoko Chiba
  • Koichiro Sera
  • Ryuji Asano
  • Takeo Sakai
Original Articles


The concentrations of 28 elements (Al, Br, Ca, Cl, Co, Cu, Cr, Fe, Ga, Hg, K, Mg, Mn, Mo, Na, Nb, Ni, P, Pb, Rb, S, Se, Si, Sr, Ti, V, Y, and Zn) were measured in mane hair by the particle-induced X-ray emission method. Except for Br, Cl, K, S, and P, the trace element concentrations in mane hair of horses are similar to literature values for human hair. The values obtained are not dependent on the horse's age, breed, and sex and could be used as reference values in the assessment of diseases and nutritional status in equines.

Index Entries

Horse mane hair particle-induced X-ray emission sex trace elements 


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  1. 1.
    Q. Feng, Y. Suzuki, and A. Hisashige, Trace element contents in hair of residents from Harbin (China), Medan (Indonesia) and Tokushima (Japan), Biol. Trace Element Res. 59, 75–86 (1997).Google Scholar
  2. 2.
    J. O. Ojo, A. F. Oluwole, M. A. Durosinmi, O. I. Asubiojo, O. A. Akanle, and N. M. Spyrou, Correlations between trace element levels in head hair and blood components of Nigerian subject. Biol. Trace, Element Res. 43–45, 453–459 (1994).Google Scholar
  3. 3.
    A. F. Oluwole, O. I. Asubiojo, D. Adekile, R. H. Filby, A. Bragg, and I. C. Grimm, Trace element distribution in the hair of some sickle cell anemia patients and controls, Biol Trace Element Res. 26–27, 479–484 (1990).CrossRefGoogle Scholar
  4. 4.
    R. F. Puchyr, D. A. Bass, R. Gajewski, et al., Preparation of hair for measurement of elements by inductively coupled plasma-mass spectrometry (ICP-MS), Biol. Trace. Element Res. 62, 167–182 (1998).Google Scholar
  5. 5.
    T. Yanai, T. Masegi, K. Ishikawa, et al., Spontaneous vascular mineralization in the brain of horses, J. Vet. Med. Sci. 58, 35–40 (1996).PubMedGoogle Scholar
  6. 6.
    E. W. Polack, J. M. King, J. F. Cummings, H. O. Mohammed, M. Birch, and T. Cronin, Concentrations of trace minerals in the spinal cord of horses with equine motor neuron disease, Am. J. Vet. Res. 61, 609–611 (2001).CrossRefGoogle Scholar
  7. 7.
    G. Stark, B. Schneider, and M. Gemeiner, Zinc and copper plasma levels in Icelandic horses with Culicoides hypersensitivity, Equine Vet. J. 33, 506–509 (2001).PubMedCrossRefGoogle Scholar
  8. 8.
    H. Brommer and M. M. van Oldruitenborgh-Oosterbaan, Iron deficiency in stabled Dutch warm blood foals, J. Vet. Intern. Med. 15, 482–485 (2001).PubMedCrossRefGoogle Scholar
  9. 9.
    H. F. Schryver, D. L. Millis, L. V. Soderholm, J. Williams, and H. F. Hintz, Metabolism of some essential minerals in ponies fed high levels of aluminum, Cornell Vet. 76, 354–360 (1986).PubMedGoogle Scholar
  10. 10.
    R. Lapatto, A. Hietamaki, and J. Raisanen, Quantitative trace element analysis of human nails with external beam PIXE, Biol. Trace Element Res. 19, 161–170 (1989).CrossRefGoogle Scholar
  11. 11.
    P. L. Leung, H. M. Huang, D. Z. Sun, and M. G. Zhu, Hair concentrations of calcium, iron, and zinc in pregnant women and effects of supplementation, Biol. Trace Element Res. 69, 269–282 (1999).Google Scholar
  12. 12.
    R. Asano, K. Suzuki, T. Otsuka, M. Otsuka, and H. Sakurai, Concentrations of toxic metals and essential minerals in the mane hair of healthy racing horses and their relation to age, J. Vet. Med. Sci. 64, 607–610 (2002).PubMedCrossRefGoogle Scholar
  13. 13.
    L. Cape and H. F. Hintz, Influence of month, color, age, corticosteroids, and dietary molybdenum on mineral concentration of equine hair, Am. J. Vet. Res. 43, 1132–1136 (1982).PubMedGoogle Scholar
  14. 14.
    M. Dunnett and P. Lees, Trace element, toxin and drug elimination in hair with particular reference to the horse, Res. Vet. Sci. 75, 89–101 (2003).PubMedCrossRefGoogle Scholar
  15. 15.
    M. A. Chyla and W. Zyrnicki, Determination of metal concentrations in animal hair by the ICP method, Biol. Trace Element Res. 75, 187–194 (2000).CrossRefGoogle Scholar
  16. 16.
    M. Chiba, Bioinorganic chemistry: a science in the spotlight—interface of chemistry, biology, agriculture and medicine, Int. J. PIXE 4, 201–216 (1994).CrossRefGoogle Scholar
  17. 17.
    K. Sera, S. Futatsugawa, and K. Matsuda, Quantitative analysis of untreated bio-samples, Nucl. Instrum. Methods Phys. Res. B 150, 226–233 (1999).CrossRefGoogle Scholar
  18. 18.
    K. Sera, S. Futatsugawa, K. Matsuda, and K. Miura, Standard-free method of quantitative analysis for bio-samples, Int. J. PIXE 6, 467–481 (1996).CrossRefGoogle Scholar
  19. 19.
    K. Asano, K. Suzuki, M. Chiba, et al., Influence of the coat color on the trace elemental status measured by particle induced X-ray emission (PIXE) in horse hair, Biol. Trace Element Res., 103, 169–176 (2005).CrossRefGoogle Scholar
  20. 20.
    M. Chiba, K. Sera, M. Hashizme, et al., Element concentrations in hair of children living in the environmentally degradated districts: the east Aral sea region, J. Radioanal. Nucl. Chem. 259, 149–152 (2004).CrossRefGoogle Scholar
  21. 21.
    R. E. Cuenca, W. J. Pories, and J Bray, Bromine levels in human serum, urine, hair, Biol. Trace Element Res. 16, 151–154 (1988).Google Scholar
  22. 22.
    L. Perrone, R. Moro, M. Caroli, R. D. Toro, and G. Gialanella, Trace elements in hair of healthy children sampled by age and sex, Biol. Trace Element Res. 51, 71–76 (1996).Google Scholar

Copyright information

© Humana Press Inc 2005

Authors and Affiliations

  • Kimi Asano
    • 1
  • Kazuyuki Suzuki
    • 1
  • Momoko Chiba
    • 2
  • Koichiro Sera
    • 3
  • Ryuji Asano
    • 1
  • Takeo Sakai
    • 1
  1. 1.Department of Veterinary Medicine, College of Bioresource SciencesNihon UniversityKanagawaJapan
  2. 2.School of MedicineJuntendo UniversityTokyoJapan
  3. 3.Cyclotron Research CenterIwate Medical UniversityIwateJapan

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