Biological Trace Element Research

, Volume 105, Issue 1–3, pp 159–170 | Cite as

Spatial distribution of lead in enamel and coronal dentine of wistar rats

  • Manish Arora
  • Sheena W. Y. Chan
  • Chris G. Ryan
  • Brendan J. Kennedy
  • D. Murray Walker
Original Articles

Abstract

Lead is one of the most hazardous environmental toxins known. The assessment of lead in dental hard tissues is important in the understanding of its to xic effects on oral tissues and in estimating exposure and body burden in individuals exposed to lead from the environment. However, current information on the uptake and distribution of lead in enamel and dentine is limited. The aim of this project was to study, at high resolution, the spatial distribution of lead in enamel and coronal dentine using an experimental rat model. A dose of 40 mg/L of lead nitrate was administered to pregnant femake rats during the periods of gestation and lactation through drinking water. First mandibular molar teeth were removed from their 15-d-old pups and the distribution of lead was studied using a nuclear microprobe (NMP). The distribution of lead in enamel and coronal dentine showed four distinct zones with significantly different mean lead concentrations (p<0.05). High levels of lead were observed in the superficial regions of enamel and in the dentine directly adjacent to the pulp. Additionally, the results confirmed that the NMP is capable of mapping the distribution of lead in teeth at micron resolutions with a detection limit of approx 1 μg/g.

Index Entries

Lead enamel dentine Wistar rats nuclear microprobe 

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References

  1. 1.
    H. L. Needleman and D. Bellinger, The health effects of low level exposure to lead, Annu. Rev. Public Health 12, 111–140 (1991).PubMedCrossRefGoogle Scholar
  2. 2.
    L. Jarup, Hazards of heavy metal contamination, Br. Med. Bull. 68, 167–182 (2003).PubMedCrossRefGoogle Scholar
  3. 3.
    J. G. Pounds, G. J. Long, and J. F. Rosen, Cellular and molecular toxicity of lead in bone, Environ. Health Perspect. 91, 17–32 (1991).PubMedCrossRefGoogle Scholar
  4. 4.
    W. H. Bowen, Exposure to metal ions and susceptibility to dental caries, J. Dent. Educ. 65, 1046–1053 (2001).PubMedGoogle Scholar
  5. 5.
    G. E. Watson, B. A. Davis, R. F. Raubertas, S. K. Pearson, and W. H. Bowen, Influence of maternal lead ingestion on caries in rat pups, Nat. Med. 3, 1024–1025 (1997).PubMedCrossRefGoogle Scholar
  6. 6.
    H. L. Needleman, C. Gunnoe, A. Leviton, et al., Deficits in psychologic and classroom performance of children with elevated dentine lead levels, N. Engl. J. Med. 300, 689–695 (1979).PubMedCrossRefGoogle Scholar
  7. 7.
    M. B. Rabinowitz, J. D. Wang, and W. T. Soong, Dentine lead and child intelligence in Taiwan, Arch. Environ. Health 46, 351–360 (1991).PubMedCrossRefGoogle Scholar
  8. 8.
    G. Winneke, L. Altmann, U. Kramer, et al., Neurobehavioral and neurophysiological observations in six year old children with low lead levels in East and West Germany, Neurotoxicology 15, 705–713 (1994).PubMedGoogle Scholar
  9. 9.
    M. Smith, T. Delves, R. Lansdown, B. Clayton, and P. Graham, The effects of lead exposure on urban children: the Institute of Child Health/Southampton Study, Dev. Med. Child. Neurol. 47(Suppl.), 1–54 (1983).Google Scholar
  10. 10.
    J. Begerow, I. Freier, M. Turfeld, U. Kramer, and L. Dunemann, Internal lead and cadmium exposure in 6-year-old children from western and eastern Germany, Int. Arch. Occup. Environ. Health 66, 243–248 (1994).PubMedCrossRefGoogle Scholar
  11. 11.
    S. R. Grobler, R. J. Rossouw, and D. Kotze, Lead in teeth of weanling rats received via the maternal drinking water, Arch. Oral. Biol. 30, 509–511 (1985).PubMedCrossRefGoogle Scholar
  12. 12.
    S. R. Grobler, R. J. Rossouw, T. J. v. W. Kotze, and I. A. Stander, The effect of airborne lead on lead levels of blood, incisors and alveolar bone of rats, Arch. Oral Biol. 36, 357–360 (1991).PubMedCrossRefGoogle Scholar
  13. 13.
    M. Kaplan, H. J. Persie, and M. Jeffcoat, Lead content of blood and deciduous teeth in lead-exposed Beagle pups, in Low Level Lead Exposure, H. L. Needleman, ed., Raven, New York, pp. 221–230 (1980).Google Scholar
  14. 14.
    R. D. Evans, P. Richner, and P. M. Outridge, Micro-spatial variations of heavy metals in the teeth of walrus as determined by laser ablation ICP-MS: the potential for reconstructing a history of metal exposure, Arch. Environ. Contam. Toxicol. 28, 55–60 (1995).PubMedCrossRefGoogle Scholar
  15. 15.
    P. Budd, J. Montgomery, A. Cox, P. Krause, B. Barreiro, and R. G. Thomas, The distribution of lead within ancient and modern human teeth: implications for long-term and historical exposure monitoring, Sci. Total Environ. 220, 121–136 (1998).PubMedCrossRefGoogle Scholar
  16. 16.
    J. E. Ericson, Enamel lead biomarker for prenatal exposure assessment, Environ. Res. 87, 136–140 (2001).PubMedCrossRefGoogle Scholar
  17. 17.
    M. M. Hoffman and I. Schour, Quantitative studies in the development of the rat molar. I. The growth pattern of the primary and secondary dentin (from birth to 500 days of age), Anat. Res. 78, 233–251 (1940).CrossRefGoogle Scholar
  18. 18.
    B. Momcilovic, Lead metabolism in lactation, Experimentia 35, 517–518 (1979).CrossRefGoogle Scholar
  19. 19.
    C. G. Ryan, D. N. Jamieson, W. L. Griffin, G. Cripps, and R. Szymanski, The new CSIRO-GEMOC nuclear microprobe: first results, performance and recent applications, Nucl. Instrum. Methods B 181, 12–19 (2001).CrossRefGoogle Scholar
  20. 20.
    C. G. Ryan, Developments in dynamic analysis for quantitative PIXE true elemental imaging, Nucl. Instrum. Methods B 181, 170–179 (2001).CrossRefGoogle Scholar
  21. 21.
    S. R. Malik and J. H. Fremlin, A study of lead distribution in human teeth using charged particle activation analysis, Caries Res. 8, 283–292 (1974).PubMedCrossRefGoogle Scholar
  22. 22.
    M. A. Crenshaw and Y. Takano, Mechanisms by which the enamel organ controls calcium entry into the developing enamel, J. Dent. Res. 61(Special Issue), 1574–1579 (1982).Google Scholar
  23. 23.
    T. Aoba and E. C. Moreno, Changes in the nature and composition of enamel mineral during porcine amelogenesis, Calcif. Tissue Int. 47, 356–364 (1990).PubMedCrossRefGoogle Scholar
  24. 24.
    N. G. Purchase and J. E. Fergusson, Lead in teeth: the influence of the tooth type and the sample within a tooth on lead levels, Sci. Total Environ. 52, 239–250 (1986).PubMedCrossRefGoogle Scholar
  25. 25.
    A. R. Ten Cate, Oral Histology: Development, Structure and Function, 5th ed., Mosby, St. Louis, MO, pp. 151 (1998).Google Scholar
  26. 26.
    P. Herr, J. Holz, and L. J. Baume, Mantle dentine in man—a quantitative microradiographic study, J. Biol. Bucc. 14, 139–146 (1986).Google Scholar
  27. 27.
    U. Stratmann, K. Schaarschmidt, H. P. Wiesmann, U. Plate, H. J. Hohling, and T. Szuwart, The mineralisation of mantle dentine and of circumpulpal dentine in the rat: an ultrastructural and element-analytical study, Anat. Embryol. 195, 289–297 (1997).PubMedCrossRefGoogle Scholar
  28. 28.
    S. R. Grobler, F. S. Theunissen, and T. J. Kotze, The relation between lead concentrations in human dential tissues and in blood, Arch. Oral. Biol. 45, 607–609 (2000).PubMedCrossRefGoogle Scholar
  29. 29.
    L. G. Petersson, A. Lodding, and G. Koch, Elemental microanalysis of enamel and dentin by secondary ion mass spectrometry (SIMS), Swed. Dent. J. 2, 41–54 (1978).PubMedGoogle Scholar
  30. 30.
    D. Kang, D. Amarasiriwardena, and A. H. Goodman, Application of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to investigate trace metal spatial distributions in human tooth enamel and dentine growth layers and pulp, Anal. Bioanal. Chem. 378, 1608–1615 (2004).PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Manish Arora
    • 1
  • Sheena W. Y. Chan
    • 1
  • Chris G. Ryan
    • 2
  • Brendan J. Kennedy
    • 3
  • D. Murray Walker
    • 1
  1. 1.Oral Pathology and Oral Medicine, Faculty of DentistryUniversity of SydneyWentworthvilleAustralia
  2. 2.Exploration and MiningCSIRONorth RydeAustralia
  3. 3.School of ChemistryUniversity of SydneySydneyAustralia

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