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Biological Trace Element Research

, Volume 105, Issue 1–3, pp 27–36 | Cite as

Influence of smoking on serum and milk malondialdehyde, superoxide dismutase, glutathione peroxidase, and antioxidant potential levels in mothers at the postpartum seventh day

  • Bahri Ermis
  • Abdulkadir Yildirim
  • Rahmi Örs
  • Ayhan Tastekin
  • Behzat Ozkan
  • Fatih Akcay
Original Articles

Abstract

The aim of the study was to investigate simultaneously serum and milk malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx) activities, and antioxidant potential (AOP) in active-smoking, passive-smoking, and nonsmoking mothers and to search if there is any difference between serum and milk oxidant/ antioxidant status caused by smoking. According to their smoking status, 60 mothers (age range: 20–35 yr) were classified into one of three groups: the active-smoking mothers (n=15), the passive-smoking mothers (n=22), and the nonsmoking mothers (n=23). Serum and milk MDA, SOD, GPx, and AOP values were determined in mothers on the postpartum seventh day by the spectrophotometric method. Serum Zn and Cu concentrations were determined by atomic absorption spectrophotometry (AAS). There was no significant difference in serum samples with respect to MDA (p=0.17), SOD (p=0.51) and AOP (p=0.36) levels, but there was a significant difference in serum GPx (p=0.002) levels among the study groups. The significant differences were also found in milk samples in terms of MDA (p=0.002) and SOD (p=0.011), but not in GPx (p=0.11) and AOP (p=0.29) levels among the study groups. No significant difference was seen in serum zinc concentration (p=0.49), but copper concentration differed significantly among the groups (p=0.005). These observations suggest that human milk is more vulnerable to oxidative stress and lipid peroxidation than serum samples in smoking mothers, even if they are passive smokers.

Index Entries

Smoking serum antioxidants malondialdehyde superoxide dismutase glutathione peroxidase human milk 

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References

  1. 1.
    J. P. Eiserich, A. van der Vliet, G. J. Handelman, et al., Dietary antioxidants and cigarette smoke-induced biomolecular damage: a complex interaction, Am. J. Clin. Nutr. 62(6 Suppl.), 1490S-1500S (1995).PubMedGoogle Scholar
  2. 2.
    S. A. Hulea, R. Olinescu, S. Nita, et al., Cigarette smoking causes biochemical changes in blood that are suggestive of oxidative stress: a case-control study, J. Environ. Pathol. Toxicol. Oncol. 14, 173–180 (1995).PubMedGoogle Scholar
  3. 3.
    S. H. Kim, J. S. Kim, H. S. Shin, et al., Influence of smoking on markers of oxidative stress and serum mineral concentrations in teenage girls in Korea, Nutrition 19, 240–243 (2003).PubMedCrossRefGoogle Scholar
  4. 4.
    H. Wurzel, C. C. Yeh, C. Gairola, et al., Oxidative damage and antioxidant status in the lungs and bronchoalveolar lavage fluid of rats exposed chronically to cigarette smoke, J. Biochem. Toxicol. 10, 11–17 (1995).PubMedGoogle Scholar
  5. 5.
    M. P. Gupta, K. L. Khanduja, and R. R. Sharma, Effect of cigarette smoke inhalation on antioxidant enzymes and lipid peroxidation in the rat, Toxicol. Lett. 41, 107–114 (1988).PubMedCrossRefGoogle Scholar
  6. 6.
    M. A. Abou-Seit, Blood antioxidant status and urine sulfate and thiocyanate levels in smokers, J. Biochem. Toxicol. 11, 133–138 (1996).CrossRefGoogle Scholar
  7. 7.
    J. Hilbert and V. Mohsenin, Adaptation of lung antioxidants to cigarette smoking in humans, Chest 110, 916–920 (1996).PubMedGoogle Scholar
  8. 8.
    R. M. Ortega, A. M. Lopez-Sobaler, R. M. Martinez, et al., Influence of smoking on vitamin E status during the third trimester and on breast-milk tocopherol concentrations in Spanish women, Am. J. Clin. Nutr. 68, 662–667 (1998).PubMedGoogle Scholar
  9. 9.
    R. M. Ortega, A. M. Lopez-Sobaler, M. E. Quintas, et al., The influence of smoking on vitamin C status during the third trimester of pregnancy and on vitamin C levels in maternal milk, J. Am. Coll. Nutr. 17, 379–384 (1998).PubMedGoogle Scholar
  10. 10.
    S. K. Jain, R. McVie, J. Duett, et al., Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes, Diabetes 38, 1539–1543 (1989).PubMedCrossRefGoogle Scholar
  11. 11.
    Y. Sun, L. W. Oberley, and Y. Li, A simple method for clinical assay of superoxide dismutase, Clin. Chem. 34, 497–500 (1988).PubMedGoogle Scholar
  12. 12.
    D. E. Paglia and W. N. Valentine, Studies on the quantiative and qualitative characterization of erythrocyte glutathione peroxidase, J. Lab. Clin. Med. 70, 158–169 (1967).PubMedGoogle Scholar
  13. 13.
    H. S. Ozturk, M. Y. Cimen, O. B. Cimen, M. Kacmaz, and I. Durak, Oxidant/antioxidant status of plasma samples from patients with rheumatoid arthritis, Rheumatol. Int. 19, 35–37 (1999).PubMedCrossRefGoogle Scholar
  14. 14.
    R. Steldinger, W. Luck, and H. Nau, Half lives of nicotine in milk of smoking mothers: implications for nursing, J. Perinat. Med. 16, 261–262 (1988).PubMedGoogle Scholar
  15. 15.
    I. Durak, S. Elgun, N. K. Bingol, et al., Effects of cigarette smoking with different tar content on erythrocyte oxidant/antioxidant status, Addict. Biol. 7, 255–258 (2002).PubMedCrossRefGoogle Scholar
  16. 16.
    K. B. Schwarz, J. M. Cox, S. Sharma, et al., Prooxidant effects of maternal smoking and formula in newborn infants, J. Pediatr. Gastroenterol. Nutr. 24, 68–74 (1997).PubMedCrossRefGoogle Scholar
  17. 17.
    Y. Sajjad, M. Leonard, and M. Doyle, Antioxidant levels in the cord blood of term fetus, J. Obstet. Gynaecol. 20, 468–471 (2000).PubMedCrossRefGoogle Scholar
  18. 18.
    M. A. Dubick and C. L. Keen, Influence of nicotine on tissue trace element concentrations and tissue antioxidant defense, Biol. Trace Element Res. 31, 97–99 (1991).Google Scholar

Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Bahri Ermis
    • 1
  • Abdulkadir Yildirim
    • 2
  • Rahmi Örs
    • 3
  • Ayhan Tastekin
    • 3
  • Behzat Ozkan
    • 3
  • Fatih Akcay
    • 2
  1. 1.Division of Neonatology, Department of Pediatrics, School of MedicineKaraelmas UniversityZonguldakTurkey
  2. 2.Department of BiochemistryAtaturk UniversityErzurumTurkey
  3. 3.Department of Pediatrics, School of MedicineAtaturk UniversityErzurumTurkey

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