Annals of Ophthalmology

, Volume 38, Issue 1, pp 43–48 | Cite as

The histopathological effect of intracameral ropivacaine in different concentrations on corneal endothelium

  • Íhsan Çaça
  • Vatan Kavak
  • Kaan Ünlü
  • Seyhmus Ari
  • Yusuf Nergis
  • Gülnür Take
Original Article


We evaluated the histopathological changes occurring in corneal endothelium after intracameral injection ropivacaine into rats. Intracamerally administered ropivacaine in 1, 0.5, and 0.1% concentrations resulted in impairment of hexagonal structure of corneal endothelial cells and inter-cellular junctions, destruction of microvilli on the cell surface, roughness of cell borders, picnotic nucleus, diffuse vacuolization, and crystalysis in mitochondria.


Bupivacaine Ropivacaine Intercellular Junction Corneal Endothelium Corneal Endothelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Patel BC, Burns TA, Crandall A, et al. A comparison of topical and retrobulbar anesthesia for cataract surgery. Ophthalmology 1996; 103:1196–1203.PubMedGoogle Scholar
  2. 2.
    Martini E, Cavallini GM, Campi L, et al. Lidocaine versus ropivacaine for topical anesthesia in cataract surgery (1). J Cataract Refract Surg 2002;28:1018–1022.PubMedCrossRefGoogle Scholar
  3. 3.
    Karp CL, Cox TA, Wagoner MD, et al. Intracameral anesthesia: a report by the American Academy of Ophthalmology. Ophthalmology 2001;108:1704–1710.PubMedCrossRefGoogle Scholar
  4. 4.
    Trivedi RH, Werner L, Apple DJ, et al. Viscoanesthesia. Part I: toxicity to corneal endothelial cells in a rabbit model. J Cataract Refract Surg 2003;29:550–555.PubMedCrossRefGoogle Scholar
  5. 5.
    Uy HS, de Jesus AA, Paray AA, Flores JD, Felizar LB. Ropivacaine-lidocaine versus bupivacaine-lidocaine for retrobulbar anesthesia in cataract surgery. J Cataract Refract Surg 2002;28:1023–1026.PubMedCrossRefGoogle Scholar
  6. 6.
    Kadonosono K, Ito N, Yazama F, et al. Effect of intracameral anesthesia on the corneal endothelium. J Cataract Refract Surg 1998; 24:1377–1381.PubMedGoogle Scholar
  7. 7.
    McLure HA, Rubin AP, Westcott M, Henderson H. A comparison of 1% ropivacaine with a mixture of 0.75% bupivacaine and 2% lignocaine for peribulbar anaesthesia. Anaesthesia 1999;54:1178–1182.PubMedCrossRefGoogle Scholar
  8. 8.
    Carpenter R. Local anesthesia toxicity: the case for ropivacaine. Am J Anesthesiol 1997;24:4–7.Google Scholar
  9. 9.
    Rutten AJ, Nancarrow C, Mather LE, Ilsley AH, Runciman WB, Upton RN. Hemodynamic and central nervous system effects of intravenous bolus doses of lidocaine, bupivacaine, and ropivacaine in sheep. Anesth Analg 1989;69:291–299.PubMedCrossRefGoogle Scholar
  10. 10.
    Moller R, Covino BG. Cardiac electrophysiologic properties of bupivacaine and lidocaine compared with those of ropivacaine, a new amide local anesthetic. Anesthesiology 1990;72:322–329.PubMedCrossRefGoogle Scholar
  11. 11.
    Huha T, Ala-Kokko TI, Salomaki T, Alahuhta S. Clinical efficacy and pharmacokinetics of 1% ropivacaine and 0.75% bupivacaine in peribulbar anaesthesia for cataract surgery. Anaesthesia 1999;54: 137–141.PubMedCrossRefGoogle Scholar
  12. 12.
    Nicholson G, Sutton B, Hall GM. Comparison of 1% ropivacaine with 0.75% bupivacaine and 2% lidocaine for peribulbar anaesthesia. Br J Anaesth 2000;84:89–91.PubMedGoogle Scholar
  13. 13.
    Woodward DK, Leung AT, Tse MW, Law RW, Lam DS, Kee WD. Peribulbar anaesthesia with 1% ropivacaine and hyaluronidase 300 IU ml-1: comparison with 0.5% bupivacaine/2% lidocaine and hyaluronidase 50 IU ml-1. Br J Anaesth 2000;85:618–620.PubMedCrossRefGoogle Scholar
  14. 14.
    Brown SM, Brooks SE, Mazow ML, et al. Cluster of diplopia cases after periocular anesthesia without hyaluronidase. J Cataract Refract Surg 1999;25:1245–1249.PubMedCrossRefGoogle Scholar
  15. 15.
    Sanford DK, Minoso y de Cal OE, Belyea DA. Response of intraocular pressure to retrobulbar and peribulbar anesthesia. Ophthalmic Surg Lasers 1998;29:815–817.PubMedGoogle Scholar
  16. 16.
    Naor J, Slomovic AR. Anesthesia modalities for cataract surgery. Curr Opin Ophthalmol 2000;11:7–11.PubMedCrossRefGoogle Scholar
  17. 17.
    Bellucci R. Anesthesia for cataract surgery. Curr Opin Ophthalmol 1999;10:36–41.PubMedCrossRefGoogle Scholar
  18. 18.
    Lo Martire N, Savastano S, Rossini L, et al. Topical anesthesia for cataract surgery with phacoemulsification: lidocaine 2% vs ropivacaine 1%. Preliminary results. Minerva Anestesiol 2002;68:529–535.PubMedGoogle Scholar
  19. 19.
    Cakmak SS, Olmez G, Nergiz Y, Unlu K, Soker S. The effects of intracameral ropivacaine on the corneal endothelium. Jpn J Ophthalmol 2005;49:267–268.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Íhsan Çaça
    • 1
  • Vatan Kavak
    • 1
  • Kaan Ünlü
    • 2
  • Seyhmus Ari
    • 3
  • Yusuf Nergis
    • 4
  • Gülnür Take
    • 5
  1. 1.Department of OphthalmologyDicle University Faculty of MedicineDiyarbakirTurkey
  2. 2.Department of OphthalmologyDicle University Faculty of MedicineDiyarbakirTurkey
  3. 3.Department of OphthalmologyDicle University Faculty of MedicineDiyarbakirTurkey
  4. 4.Department of Histology and EmbryologyDicle University Faculty of MedicineDiyarbakirTurkey
  5. 5.Department of Histology and EmbryologyGazi University Faculty of MedicineDiyarbakirTurkey

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