Abstract
Kaempferia galanga is an important medicinal plant that is facing threat of extinction owing to indiscriminate and unsustainable harvesting in the wild. Conventional breeding is difficult in this plant, and in vitro multiplication is important to conservation and propagation. Leaf and rhizome explants of Kaempferia were aseptically cultured on MS medium with various combinations of indole-3-acetic acid (IAA), benzyl amino purine (BAP), napthalene acetic acid (NAA), 2-4-dichlorophenoxy acetic acid (2,4-D) and kinetin at concentrations ranging from 0.5 to 2.5 mg/L. High-frequency organogenesis and multiple shoot regeneration was induced from rhizome explants on MS medium supplemented with 0.5 mg/L of IAA and 2.5 mg/L of BAP. Rooting was induced in MS medium with 0.5 mg/L of IAA and 2 mg/L of BAP.
Similar content being viewed by others
References
Rajasekharan, P. E. and Ganeshan, S. (2002), J. Med. Aromat. Plant Sci. 24, 132–147.
Wong, K. C., Ong, K. S., and Lim, C. L. (1992), Flavour Fragrance J. 7(5), 263–266.
Revikumar, K. and Ved, D. K. (2000), in Red Listed Medicinal Plants of Conservation Concern in Southern India, 1st ed., pp. 212–214.
Murashige, T. and Skoog, F. (1962), Physiol. Plant 15, 473–497.
Rao, N. K. S., Narayanaswamy, S., Chacko, E. K., and Doreswamy, R. (1982), Proc. Indian Acad. Sci. (Plant Sci.) 91, 37–41.
Nadgaude, R. S., Kulkarni, D. D., Mascarenhas, A. F., and Jagannathan, V. (1980), in Proceedings of the Symposium on Plant Tissue Culture, Genetic Manipulation and Somatic Hybridization of Plant Cells, Pao, P. S., Heble, M. R., and Chandra, M. S., eds., BARC, Bombay, India, pp. 358–361.
Nirmalbabu, K., Samsudeen, K., and Ratnambal, M. J. (1992), Plant Cell Tissue Organ Cult. 29, 71–74.
Yasuda, K., Tsuda, T., Shimizu, H., and Sugaya, A. (1988), Planta Med. 54, 75–79.
Vincent, K. A., Bejoy, M., Hariharan, M., and Mathew, K. M. (1991), Indian J. Plant Physiol. 34, 396–400.
Anand, P. H. M., Hariharan, K. N., Martin, K. P., and Hariharan, M. (1997), Phytomorphology 47, 281–286.
Geetha, S. P., Manjula, C., John, C. Z., Minoo, D., Nirmal Babu, K., and Ravindran, P. H. (1993), J. Spices Aromat. Crops 6(2), 129–135.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Swapna, T.S., Binitha, M. & Manju, T.S. In vitro multiplication in Kaempferia galanga linn. Appl Biochem Biotechnol 118, 233–241 (2004). https://doi.org/10.1385/ABAB:118:1-3:233
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1385/ABAB:118:1-3:233